Morphological and Molecular (28s rDNA) Characterization of Dicrocoelium dendriticum Isolates from Sheep, Goat and Cattle in Mazandaran Province, Iran
Background and purpose: Dicrocoeliasis is a prevalent parasite in ruminants caused by different species of Dicrocoelium spp. This parasite lives in bile duct and gallbladder of ruminants and accidentally in human. The economic significance of dicroceliasis is due to direct damage to the liver and, c...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2018-04-01
|
| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-11124-en.html |
| _version_ | 1797943792766025728 |
|---|---|
| author | Simin Bari Mohsen Arbabi Pooria Gill Mehdi Sharif Hajar Ziaei Hezarjaribi Samira Dodangeh Abbas Alizadeh Zeinab Hedayati Shahabeddin Sarvi |
| author_facet | Simin Bari Mohsen Arbabi Pooria Gill Mehdi Sharif Hajar Ziaei Hezarjaribi Samira Dodangeh Abbas Alizadeh Zeinab Hedayati Shahabeddin Sarvi |
| author_sort | Simin Bari |
| collection | DOAJ |
| description | Background and purpose: Dicrocoeliasis is a prevalent parasite in ruminants caused by different species of Dicrocoelium spp. This parasite lives in bile duct and gallbladder of ruminants and accidentally in human. The economic significance of dicroceliasis is due to direct damage to the liver and, consequently, to the loss of significant amounts of protein. The present study aimed to investigate the morphometric and molecular (PCR and PCR-RFLP) parameters of Dicrocoelium species isolates from sheep, goat, and cattle in Mazandaran province, Iran during 2013-2015.
Materials and methods: In this descriptive study, 50 trematodes isolated from livers of sheep (n= 20), goat (n= 20), and cattle (n= 10) were collected form abattoirs in Mazandaran province. Adult worms were studied by morphometric and molecular methods on 28s rDNA. The RFLP technique was used to identify the genus. Some samples were also sequenced.
Results: All isolates of three hosts (sheep, goat, and cattle) showed significant differences in many morphological parameters. According to characterization of testicles that is a remarkable characteristic for differential diagnosis of Dicrocoelium species, they were in tandem position in all isolates. Moreover, no significant difference was observed in their size in different hosts. Four bands, including 116, 145, 393, and 409 bp were produced from PCR method after treatment by enzyme. In molecular technique, there were no any significant differences but in morphometric analysis, some differences were found.
Conclusion: The molecular and morphometric results indicated that D. dendriticum is the only species infecting sheep, goat, and cattle in Mazandaran province. |
| first_indexed | 2024-04-10T20:29:49Z |
| format | Article |
| id | doaj.art-a10a8d49c0904a4293f24b1ffcafdcb0 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:29:49Z |
| publishDate | 2018-04-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-a10a8d49c0904a4293f24b1ffcafdcb02023-01-25T07:35:55ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792018-04-01281593845Morphological and Molecular (28s rDNA) Characterization of Dicrocoelium dendriticum Isolates from Sheep, Goat and Cattle in Mazandaran Province, IranSimin Bari0Mohsen Arbabi1Pooria Gill2Mehdi Sharif3Hajar Ziaei Hezarjaribi4Samira Dodangeh5Abbas Alizadeh6Zeinab Hedayati7Shahabeddin Sarvi8 Background and purpose: Dicrocoeliasis is a prevalent parasite in ruminants caused by different species of Dicrocoelium spp. This parasite lives in bile duct and gallbladder of ruminants and accidentally in human. The economic significance of dicroceliasis is due to direct damage to the liver and, consequently, to the loss of significant amounts of protein. The present study aimed to investigate the morphometric and molecular (PCR and PCR-RFLP) parameters of Dicrocoelium species isolates from sheep, goat, and cattle in Mazandaran province, Iran during 2013-2015. Materials and methods: In this descriptive study, 50 trematodes isolated from livers of sheep (n= 20), goat (n= 20), and cattle (n= 10) were collected form abattoirs in Mazandaran province. Adult worms were studied by morphometric and molecular methods on 28s rDNA. The RFLP technique was used to identify the genus. Some samples were also sequenced. Results: All isolates of three hosts (sheep, goat, and cattle) showed significant differences in many morphological parameters. According to characterization of testicles that is a remarkable characteristic for differential diagnosis of Dicrocoelium species, they were in tandem position in all isolates. Moreover, no significant difference was observed in their size in different hosts. Four bands, including 116, 145, 393, and 409 bp were produced from PCR method after treatment by enzyme. In molecular technique, there were no any significant differences but in morphometric analysis, some differences were found. Conclusion: The molecular and morphometric results indicated that D. dendriticum is the only species infecting sheep, goat, and cattle in Mazandaran province.http://jmums.mazums.ac.ir/article-1-11124-en.htmldicrocoeliummorphology28s rdnapcr-rflp |
| spellingShingle | Simin Bari Mohsen Arbabi Pooria Gill Mehdi Sharif Hajar Ziaei Hezarjaribi Samira Dodangeh Abbas Alizadeh Zeinab Hedayati Shahabeddin Sarvi Morphological and Molecular (28s rDNA) Characterization of Dicrocoelium dendriticum Isolates from Sheep, Goat and Cattle in Mazandaran Province, Iran Journal of Mazandaran University of Medical Sciences dicrocoelium morphology 28s rdna pcr-rflp |
| title | Morphological and Molecular (28s rDNA) Characterization of Dicrocoelium dendriticum Isolates from Sheep, Goat and Cattle in Mazandaran Province, Iran |
| title_full | Morphological and Molecular (28s rDNA) Characterization of Dicrocoelium dendriticum Isolates from Sheep, Goat and Cattle in Mazandaran Province, Iran |
| title_fullStr | Morphological and Molecular (28s rDNA) Characterization of Dicrocoelium dendriticum Isolates from Sheep, Goat and Cattle in Mazandaran Province, Iran |
| title_full_unstemmed | Morphological and Molecular (28s rDNA) Characterization of Dicrocoelium dendriticum Isolates from Sheep, Goat and Cattle in Mazandaran Province, Iran |
| title_short | Morphological and Molecular (28s rDNA) Characterization of Dicrocoelium dendriticum Isolates from Sheep, Goat and Cattle in Mazandaran Province, Iran |
| title_sort | morphological and molecular 28s rdna characterization of dicrocoelium dendriticum isolates from sheep goat and cattle in mazandaran province iran |
| topic | dicrocoelium morphology 28s rdna pcr-rflp |
| url | http://jmums.mazums.ac.ir/article-1-11124-en.html |
| work_keys_str_mv | AT siminbari morphologicalandmolecular28srdnacharacterizationofdicrocoeliumdendriticumisolatesfromsheepgoatandcattleinmazandaranprovinceiran AT mohsenarbabi morphologicalandmolecular28srdnacharacterizationofdicrocoeliumdendriticumisolatesfromsheepgoatandcattleinmazandaranprovinceiran AT pooriagill morphologicalandmolecular28srdnacharacterizationofdicrocoeliumdendriticumisolatesfromsheepgoatandcattleinmazandaranprovinceiran AT mehdisharif morphologicalandmolecular28srdnacharacterizationofdicrocoeliumdendriticumisolatesfromsheepgoatandcattleinmazandaranprovinceiran AT hajarziaeihezarjaribi morphologicalandmolecular28srdnacharacterizationofdicrocoeliumdendriticumisolatesfromsheepgoatandcattleinmazandaranprovinceiran AT samiradodangeh morphologicalandmolecular28srdnacharacterizationofdicrocoeliumdendriticumisolatesfromsheepgoatandcattleinmazandaranprovinceiran AT abbasalizadeh morphologicalandmolecular28srdnacharacterizationofdicrocoeliumdendriticumisolatesfromsheepgoatandcattleinmazandaranprovinceiran AT zeinabhedayati morphologicalandmolecular28srdnacharacterizationofdicrocoeliumdendriticumisolatesfromsheepgoatandcattleinmazandaranprovinceiran AT shahabeddinsarvi morphologicalandmolecular28srdnacharacterizationofdicrocoeliumdendriticumisolatesfromsheepgoatandcattleinmazandaranprovinceiran |