A Novel Route for Double-Layered Encapsulation of <i>Streptomyces fulvissimus</i> Uts22 by Alginate–Arabic Gum for Controlling of <i>Pythium aphanidermatum</i> in Cucumber
Damping-off disease due to <i>Pythium aphanidermatum</i> is one of the most harmful diseases of cucumber. One of the critical issues in the field of biological control is the establishment of a link between the beneficial bacteria screened in the laboratory and its industrial application...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/3/655 |
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| author | Roohallah Saberi Riseh Mojde Moradi Pour Essaid Ait Barka |
| author_facet | Roohallah Saberi Riseh Mojde Moradi Pour Essaid Ait Barka |
| author_sort | Roohallah Saberi Riseh |
| collection | DOAJ |
| description | Damping-off disease due to <i>Pythium aphanidermatum</i> is one of the most harmful diseases of cucumber. One of the critical issues in the field of biological control is the establishment of a link between the beneficial bacteria screened in the laboratory and its industrial application. Therefore, when developing biocontrol agents, it is necessary to study the optimization of mass production conditions and to select a suitable carrier for their final formulation. In this study, an attempt was made to provide a suitable formulation for a <i>Streptomyces fulvissimus</i> Uts22 strain based on alginate–Arabic gum and nanoparticles (SiO<sub>2</sub> and TiO<sub>2</sub>) with a layer-by-layer technique. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) studies showed that when Arabic gum was added to the composition, an electrostatic interaction occurred between alginate and Arabic gum. The scanning electron microscope image of beads show a cubic shape and good dispersion of microcapsules. The encapsulation efficiency in the prepared formulation was reported to be 94%. The maximum release of bacteria from the capsule was recorded on the 35th day of storage, about 109 CFU/gr. The greenhouse experiments showed that encapsulated bacteria resulted in a 95% reduction in damping-off disease of cucumber and showed more potential effects on increasing plant growth traits than free bacteria. This encapsulation strategy can be considered as a suitable alternative for future applications in the agricultural field. |
| first_indexed | 2024-03-09T13:55:16Z |
| format | Article |
| id | doaj.art-a10b22a1df844197ad8e8f59b6fae8f3 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T13:55:16Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-a10b22a1df844197ad8e8f59b6fae8f32023-11-30T20:44:37ZengMDPI AGAgronomy2073-43952022-03-0112365510.3390/agronomy12030655A Novel Route for Double-Layered Encapsulation of <i>Streptomyces fulvissimus</i> Uts22 by Alginate–Arabic Gum for Controlling of <i>Pythium aphanidermatum</i> in CucumberRoohallah Saberi Riseh0Mojde Moradi Pour1Essaid Ait Barka2Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan 7718897111, IranDepartment of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan 7718897111, IranInduced Resistance and Plant BioProtection Research Unit, UFR Sciences, UPRES EA 4707-USC INRAeE1488, University of Reims Champagne-Ardenne, 51687 Reims, FranceDamping-off disease due to <i>Pythium aphanidermatum</i> is one of the most harmful diseases of cucumber. One of the critical issues in the field of biological control is the establishment of a link between the beneficial bacteria screened in the laboratory and its industrial application. Therefore, when developing biocontrol agents, it is necessary to study the optimization of mass production conditions and to select a suitable carrier for their final formulation. In this study, an attempt was made to provide a suitable formulation for a <i>Streptomyces fulvissimus</i> Uts22 strain based on alginate–Arabic gum and nanoparticles (SiO<sub>2</sub> and TiO<sub>2</sub>) with a layer-by-layer technique. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) studies showed that when Arabic gum was added to the composition, an electrostatic interaction occurred between alginate and Arabic gum. The scanning electron microscope image of beads show a cubic shape and good dispersion of microcapsules. The encapsulation efficiency in the prepared formulation was reported to be 94%. The maximum release of bacteria from the capsule was recorded on the 35th day of storage, about 109 CFU/gr. The greenhouse experiments showed that encapsulated bacteria resulted in a 95% reduction in damping-off disease of cucumber and showed more potential effects on increasing plant growth traits than free bacteria. This encapsulation strategy can be considered as a suitable alternative for future applications in the agricultural field.https://www.mdpi.com/2073-4395/12/3/655alginateArabic gumencapsulationPythiumcucumberbiological control |
| spellingShingle | Roohallah Saberi Riseh Mojde Moradi Pour Essaid Ait Barka A Novel Route for Double-Layered Encapsulation of <i>Streptomyces fulvissimus</i> Uts22 by Alginate–Arabic Gum for Controlling of <i>Pythium aphanidermatum</i> in Cucumber Agronomy alginate Arabic gum encapsulation Pythium cucumber biological control |
| title | A Novel Route for Double-Layered Encapsulation of <i>Streptomyces fulvissimus</i> Uts22 by Alginate–Arabic Gum for Controlling of <i>Pythium aphanidermatum</i> in Cucumber |
| title_full | A Novel Route for Double-Layered Encapsulation of <i>Streptomyces fulvissimus</i> Uts22 by Alginate–Arabic Gum for Controlling of <i>Pythium aphanidermatum</i> in Cucumber |
| title_fullStr | A Novel Route for Double-Layered Encapsulation of <i>Streptomyces fulvissimus</i> Uts22 by Alginate–Arabic Gum for Controlling of <i>Pythium aphanidermatum</i> in Cucumber |
| title_full_unstemmed | A Novel Route for Double-Layered Encapsulation of <i>Streptomyces fulvissimus</i> Uts22 by Alginate–Arabic Gum for Controlling of <i>Pythium aphanidermatum</i> in Cucumber |
| title_short | A Novel Route for Double-Layered Encapsulation of <i>Streptomyces fulvissimus</i> Uts22 by Alginate–Arabic Gum for Controlling of <i>Pythium aphanidermatum</i> in Cucumber |
| title_sort | novel route for double layered encapsulation of i streptomyces fulvissimus i uts22 by alginate arabic gum for controlling of i pythium aphanidermatum i in cucumber |
| topic | alginate Arabic gum encapsulation Pythium cucumber biological control |
| url | https://www.mdpi.com/2073-4395/12/3/655 |
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