Green biosynthesized silver nanoparticles using Acalypha wilkesiana extract control root-knot nematode
Nanoparticles nowadays are an effective control agent against most phytopathogens. However, there are many reports on their antimicrobial activity and little as a nematicide. In this study, green biosynthesis of silver nanoparticles (Ag NPs) using Acalypha wilkesiana aqueous leaf extract was achieve...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364721001774 |
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| author | Ahmed A. Heflish Amira E. Hanfy Mohammad Javed Ansari Eldessoky S. Dessoky Attia O. Attia Moustafa M. Elshaer Mohamed K. Gaber Ahmed Kordy Ahmed S. Doma Ahmed Abdelkhalek Said I. Behiry |
| author_facet | Ahmed A. Heflish Amira E. Hanfy Mohammad Javed Ansari Eldessoky S. Dessoky Attia O. Attia Moustafa M. Elshaer Mohamed K. Gaber Ahmed Kordy Ahmed S. Doma Ahmed Abdelkhalek Said I. Behiry |
| author_sort | Ahmed A. Heflish |
| collection | DOAJ |
| description | Nanoparticles nowadays are an effective control agent against most phytopathogens. However, there are many reports on their antimicrobial activity and little as a nematicide. In this study, green biosynthesis of silver nanoparticles (Ag NPs) using Acalypha wilkesiana aqueous leaf extract was achieved. X-ray diffraction patterns confirmed the crystalline structure with face-centred cubic (fcc), where is the size was nearly 20 nm. SEM images of the Ag NPs show the spherical shape and in the range from 10 to 30 nm. Various functional groups, capping, and stability agents were identified using Fourier transmission infrared spectroscopy (FTIR). The nematicidal activity of biosynthesized Ag NPs conc. 25, 50, and 100 µg/mL were evaluated in vitro against root-knot nematode (Meloidogyne incognita), egg hatching (6 days after) and movement after 24 and 48 h. The Ag NPs (100 µg/ml) application after 48 h was the most effective treatment that showed 53.3% of nematode mortality. Overall, the efficiency of bio-Ag NPs reduced the nematode activity, mortality, egg hatching, and movement of larvae. To our knowledge, this is the first report of nematicidal action of biosynthesized Ag NPs using A. wilkesiana aqueous extract, and it could be recommended to manage the plant-parasitic nematode as it is simple, stable, cost-effective and keep the environment safe. |
| first_indexed | 2024-12-22T05:44:01Z |
| format | Article |
| id | doaj.art-a10c0084fe0c4169b1f671985806818d |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-22T05:44:01Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-a10c0084fe0c4169b1f671985806818d2022-12-21T18:37:07ZengElsevierJournal of King Saud University: Science1018-36472021-09-01336101516Green biosynthesized silver nanoparticles using Acalypha wilkesiana extract control root-knot nematodeAhmed A. Heflish0Amira E. Hanfy1Mohammad Javed Ansari2Eldessoky S. Dessoky3Attia O. Attia4Moustafa M. Elshaer5Mohamed K. Gaber6Ahmed Kordy7Ahmed S. Doma8Ahmed Abdelkhalek9Said I. Behiry10Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, EgyptAgricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, EgyptDepartment of Botany, Hindu College Moradabad (Mahatma Jyotiba Phule Rohilkhand University Bareilly) India-244001; Corresponding authors.Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Microbiology at Specialized Medical Hospital, Mansoura University, EgyptPlant Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, EgyptPlant Protection Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, EgyptPolymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications, New Borg Al-Arab City, 21934 Alexandria, EgyptPlant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, Egypt; Corresponding authors.Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, EgyptNanoparticles nowadays are an effective control agent against most phytopathogens. However, there are many reports on their antimicrobial activity and little as a nematicide. In this study, green biosynthesis of silver nanoparticles (Ag NPs) using Acalypha wilkesiana aqueous leaf extract was achieved. X-ray diffraction patterns confirmed the crystalline structure with face-centred cubic (fcc), where is the size was nearly 20 nm. SEM images of the Ag NPs show the spherical shape and in the range from 10 to 30 nm. Various functional groups, capping, and stability agents were identified using Fourier transmission infrared spectroscopy (FTIR). The nematicidal activity of biosynthesized Ag NPs conc. 25, 50, and 100 µg/mL were evaluated in vitro against root-knot nematode (Meloidogyne incognita), egg hatching (6 days after) and movement after 24 and 48 h. The Ag NPs (100 µg/ml) application after 48 h was the most effective treatment that showed 53.3% of nematode mortality. Overall, the efficiency of bio-Ag NPs reduced the nematode activity, mortality, egg hatching, and movement of larvae. To our knowledge, this is the first report of nematicidal action of biosynthesized Ag NPs using A. wilkesiana aqueous extract, and it could be recommended to manage the plant-parasitic nematode as it is simple, stable, cost-effective and keep the environment safe.http://www.sciencedirect.com/science/article/pii/S1018364721001774Ag NPsGreen biosynthesisAcalypha wilkesianaFTIRSEMNematicidal activity |
| spellingShingle | Ahmed A. Heflish Amira E. Hanfy Mohammad Javed Ansari Eldessoky S. Dessoky Attia O. Attia Moustafa M. Elshaer Mohamed K. Gaber Ahmed Kordy Ahmed S. Doma Ahmed Abdelkhalek Said I. Behiry Green biosynthesized silver nanoparticles using Acalypha wilkesiana extract control root-knot nematode Journal of King Saud University: Science Ag NPs Green biosynthesis Acalypha wilkesiana FTIR SEM Nematicidal activity |
| title | Green biosynthesized silver nanoparticles using Acalypha wilkesiana extract control root-knot nematode |
| title_full | Green biosynthesized silver nanoparticles using Acalypha wilkesiana extract control root-knot nematode |
| title_fullStr | Green biosynthesized silver nanoparticles using Acalypha wilkesiana extract control root-knot nematode |
| title_full_unstemmed | Green biosynthesized silver nanoparticles using Acalypha wilkesiana extract control root-knot nematode |
| title_short | Green biosynthesized silver nanoparticles using Acalypha wilkesiana extract control root-knot nematode |
| title_sort | green biosynthesized silver nanoparticles using acalypha wilkesiana extract control root knot nematode |
| topic | Ag NPs Green biosynthesis Acalypha wilkesiana FTIR SEM Nematicidal activity |
| url | http://www.sciencedirect.com/science/article/pii/S1018364721001774 |
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