Interleukins Profiling in Umbilical Cord Mesenchymal Stem Cell-Derived Secretome
Angliana Chouw,1,2 Cynthia Retna Sartika,2 Tiana Milanda,3 Ahmad Faried4 1Doctoral Program, Faculty of Pharmacy Universitas Padjadjaran, Jatinangor, West Java, Indonesia; 2Research and Development, Prodia StemCell Indonesia, Jakarta, DKI, Jakarta, Indonesia; 3Department of Biological Pharmacy, Facul...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-04-01
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| Series: | Stem Cells and Cloning: Advances and Applications |
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| Online Access: | https://www.dovepress.com/interleukins-profiling-in-umbilical-cord-mesenchymal-stem-cell-derived-peer-reviewed-fulltext-article-SCCAA |
| _version_ | 1828848476024209408 |
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| author | Chouw A Sartika CR Milanda T Faried A |
| author_facet | Chouw A Sartika CR Milanda T Faried A |
| author_sort | Chouw A |
| collection | DOAJ |
| description | Angliana Chouw,1,2 Cynthia Retna Sartika,2 Tiana Milanda,3 Ahmad Faried4 1Doctoral Program, Faculty of Pharmacy Universitas Padjadjaran, Jatinangor, West Java, Indonesia; 2Research and Development, Prodia StemCell Indonesia, Jakarta, DKI, Jakarta, Indonesia; 3Department of Biological Pharmacy, Faculty of Pharmacy Universitas Padjadjaran, Jatinangor, West Java, Indonesia; 4Stem Cell Working Group, Faculty of Medicine Universitas Padjadjaran, Dr. Hasan Sadikin Hospital, Bandung, West Java, IndonesiaCorrespondence: Angliana Chouw; Tiana Milanda, Email angliana@prostem.co.id; tiana.milanda@unpad.ac.idBackground: Umbilical cord mesenchymal stem cells (UC-MSCs)-derived secretome is currently used in regenerative therapy. MSCs are believed to secrete a wide spectrum of bioactive molecules which give paracrine effects in immunomodulation and regenerative capacities. One group that was found in secretome is interleukins (ILs), a cytokine that plays an essential role in the process of proliferation, differentiation, maturation, migration, and adhesion of immune cells. However, as there are many types of ILs, the profile of ILs in the UC-MSCs-derived secretome has been limitedly reported. Therefore, in this study, we would like to profile and detect the interleukin concentration secreted by UC-MSCs.Methods: UC-MSCs-derived secretome was collected from UC-MSCs passage 5 after 24- and 48-hour incubation (n=9). Secretome was filtered using 0.2 μm and stored at − 80°C for further detection. All samples were normalized before the interleukin (IL-2, IL-4, IL-6, IL-9, IL-10, IL-12, IL-17A) detection using a MACSPlex Cytokine Kit.Results: The IL-6 has the highest concentration among other interleukins in both groups and increases significantly (p< 0.003) after incubation for 48 hours. The pro-inflammatory factors are decreasing while anti-inflammatory factors are increasing after 48-hour incubation.Discussion: Our studies show that the UC-MSCs secrete pro- and anti-inflammatory interleukins. The concentration of anti-inflammatory interleukins shows to be increasing, while the pro-inflammatory interleukins are decreasing within the longer incubation time, but this not be applicable for IL-10 and IL-6. IL-6 has the highest concentration among other ILs. These results may provide important clues regarding when is the right time for secretome to be used in therapy patients, because all the molecules in the secretome can lead to many clinical manifestations.Keywords: secretome, mesenchymal stem cell, interleukin, conditioned medium, umbilical cord |
| first_indexed | 2024-12-12T22:30:52Z |
| format | Article |
| id | doaj.art-a10ea449e03a4ad6ac0a5c79ef7b2676 |
| institution | Directory Open Access Journal |
| issn | 1178-6957 |
| language | English |
| last_indexed | 2024-12-12T22:30:52Z |
| publishDate | 2022-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Stem Cells and Cloning: Advances and Applications |
| spelling | doaj.art-a10ea449e03a4ad6ac0a5c79ef7b26762022-12-22T00:09:36ZengDove Medical PressStem Cells and Cloning: Advances and Applications1178-69572022-04-01Volume 151974519Interleukins Profiling in Umbilical Cord Mesenchymal Stem Cell-Derived SecretomeChouw ASartika CRMilanda TFaried AAngliana Chouw,1,2 Cynthia Retna Sartika,2 Tiana Milanda,3 Ahmad Faried4 1Doctoral Program, Faculty of Pharmacy Universitas Padjadjaran, Jatinangor, West Java, Indonesia; 2Research and Development, Prodia StemCell Indonesia, Jakarta, DKI, Jakarta, Indonesia; 3Department of Biological Pharmacy, Faculty of Pharmacy Universitas Padjadjaran, Jatinangor, West Java, Indonesia; 4Stem Cell Working Group, Faculty of Medicine Universitas Padjadjaran, Dr. Hasan Sadikin Hospital, Bandung, West Java, IndonesiaCorrespondence: Angliana Chouw; Tiana Milanda, Email angliana@prostem.co.id; tiana.milanda@unpad.ac.idBackground: Umbilical cord mesenchymal stem cells (UC-MSCs)-derived secretome is currently used in regenerative therapy. MSCs are believed to secrete a wide spectrum of bioactive molecules which give paracrine effects in immunomodulation and regenerative capacities. One group that was found in secretome is interleukins (ILs), a cytokine that plays an essential role in the process of proliferation, differentiation, maturation, migration, and adhesion of immune cells. However, as there are many types of ILs, the profile of ILs in the UC-MSCs-derived secretome has been limitedly reported. Therefore, in this study, we would like to profile and detect the interleukin concentration secreted by UC-MSCs.Methods: UC-MSCs-derived secretome was collected from UC-MSCs passage 5 after 24- and 48-hour incubation (n=9). Secretome was filtered using 0.2 μm and stored at − 80°C for further detection. All samples were normalized before the interleukin (IL-2, IL-4, IL-6, IL-9, IL-10, IL-12, IL-17A) detection using a MACSPlex Cytokine Kit.Results: The IL-6 has the highest concentration among other interleukins in both groups and increases significantly (p< 0.003) after incubation for 48 hours. The pro-inflammatory factors are decreasing while anti-inflammatory factors are increasing after 48-hour incubation.Discussion: Our studies show that the UC-MSCs secrete pro- and anti-inflammatory interleukins. The concentration of anti-inflammatory interleukins shows to be increasing, while the pro-inflammatory interleukins are decreasing within the longer incubation time, but this not be applicable for IL-10 and IL-6. IL-6 has the highest concentration among other ILs. These results may provide important clues regarding when is the right time for secretome to be used in therapy patients, because all the molecules in the secretome can lead to many clinical manifestations.Keywords: secretome, mesenchymal stem cell, interleukin, conditioned medium, umbilical cordhttps://www.dovepress.com/interleukins-profiling-in-umbilical-cord-mesenchymal-stem-cell-derived-peer-reviewed-fulltext-article-SCCAAsecretomemesenchymal stem cellinterleukinconditioned mediumumbilical cord |
| spellingShingle | Chouw A Sartika CR Milanda T Faried A Interleukins Profiling in Umbilical Cord Mesenchymal Stem Cell-Derived Secretome Stem Cells and Cloning: Advances and Applications secretome mesenchymal stem cell interleukin conditioned medium umbilical cord |
| title | Interleukins Profiling in Umbilical Cord Mesenchymal Stem Cell-Derived Secretome |
| title_full | Interleukins Profiling in Umbilical Cord Mesenchymal Stem Cell-Derived Secretome |
| title_fullStr | Interleukins Profiling in Umbilical Cord Mesenchymal Stem Cell-Derived Secretome |
| title_full_unstemmed | Interleukins Profiling in Umbilical Cord Mesenchymal Stem Cell-Derived Secretome |
| title_short | Interleukins Profiling in Umbilical Cord Mesenchymal Stem Cell-Derived Secretome |
| title_sort | interleukins profiling in umbilical cord mesenchymal stem cell derived secretome |
| topic | secretome mesenchymal stem cell interleukin conditioned medium umbilical cord |
| url | https://www.dovepress.com/interleukins-profiling-in-umbilical-cord-mesenchymal-stem-cell-derived-peer-reviewed-fulltext-article-SCCAA |
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