Protective effect of ZYMT, a traditional Chinese patent medicine in a mouse model of retinitis pigmentosa
Retinitis pigmentosa (RP) is the most common genetic disorder that causes blindness. At present, there exists no remedy for the disease. The aim of the current research was to investigate the protective effect of Zhangyanming Tablets (ZYMT) in a mouse model of RP, and explore the underlying mechanis...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223003682 |
| _version_ | 1797837182311858176 |
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| author | Ziyang Huang Qiuling Huang Kai Xu Lina Liang Yamin Li Wei Zhou Na Ning Jiewen Zhou Jiajia Hu Shanshan Liu Lei Dang |
| author_facet | Ziyang Huang Qiuling Huang Kai Xu Lina Liang Yamin Li Wei Zhou Na Ning Jiewen Zhou Jiajia Hu Shanshan Liu Lei Dang |
| author_sort | Ziyang Huang |
| collection | DOAJ |
| description | Retinitis pigmentosa (RP) is the most common genetic disorder that causes blindness. At present, there exists no remedy for the disease. The aim of the current research was to investigate the protective effect of Zhangyanming Tablets (ZYMT) in a mouse model of RP, and explore the underlying mechanism. Eighty RP mice were randomly divided into two groups. The mice in ZYMT group were administered with ZYMT suspension(0.0378 g/mL), while the mice in model group were given the same volume of distilled water. At day 7 and day 14 after intervention, electroretinogram (ERG), fundus photography, and histological examination were used to assess the retinal function and structure. TUNEL, immunofluorescence and qPCR were used to evaluate cell apoptosis and expressions of Sirt1, Iba1, Bcl-2, Bax and Caspase-3. A significantly shortened latency of ERG waves was observed in ZYMT-treated mice, in comparison to those in the model group (P < 0.05). Histologically, ultrastructure of the retina was better preserved, and the outer nuclear layer (ONL) exhibited marked increase in thickness and cell count in ZYMP group (P < 0.05). The apoptosis rate was decreased markedly in ZYMT group. Immunofluorescence analysis showed that the expressions of Iba1 and Bcl-2 in the retina were increased, Bax and Caspase-3 were decreased after ZYMT intervention, while the qPCR revealed that the expressions of Iba1 and Sirt1 were significantly increased (P < 0.05). This study indicated that ZYMT has protective effect on retinal function and morphology of inherited RP mice in the early stage, possibly mediated via the regulation of antioxidant and anti-/pro-apoptotic factors expressions. |
| first_indexed | 2024-04-09T15:21:19Z |
| format | Article |
| id | doaj.art-a11065de62b04cfca3e634846f56793e |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-09T15:21:19Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-a11065de62b04cfca3e634846f56793e2023-04-29T14:45:53ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-06-01162114580Protective effect of ZYMT, a traditional Chinese patent medicine in a mouse model of retinitis pigmentosaZiyang Huang0Qiuling Huang1Kai Xu2Lina Liang3Yamin Li4Wei Zhou5Na Ning6Jiewen Zhou7Jiajia Hu8Shanshan Liu9Lei Dang10Eye Hospital, China Academy of Chinese Medical Sciences, Beijing, ChinaGuangdong Development Engineering Laboratory of Southern Chinese Herbal Drugs, Guangzhou, ChinaEye Hospital, China Academy of Chinese Medical Sciences, Beijing, ChinaEye Hospital, China Academy of Chinese Medical Sciences, Beijing, China; Correspondence to: Eye Hospital, China Academy of Chinese Medical Sciences, Beijing 100040, China.Eye Hospital, China Academy of Chinese Medical Sciences, Beijing, ChinaEye Hospital, China Academy of Chinese Medical Sciences, Beijing, ChinaGuangdong Development Engineering Laboratory of Southern Chinese Herbal Drugs, Guangzhou, ChinaGuangdong Development Engineering Laboratory of Southern Chinese Herbal Drugs, Guangzhou, ChinaGuangdong Development Engineering Laboratory of Southern Chinese Herbal Drugs, Guangzhou, ChinaGuangdong Development Engineering Laboratory of Southern Chinese Herbal Drugs, Guangzhou, ChinaGuangdong Development Engineering Laboratory of Southern Chinese Herbal Drugs, Guangzhou, ChinaRetinitis pigmentosa (RP) is the most common genetic disorder that causes blindness. At present, there exists no remedy for the disease. The aim of the current research was to investigate the protective effect of Zhangyanming Tablets (ZYMT) in a mouse model of RP, and explore the underlying mechanism. Eighty RP mice were randomly divided into two groups. The mice in ZYMT group were administered with ZYMT suspension(0.0378 g/mL), while the mice in model group were given the same volume of distilled water. At day 7 and day 14 after intervention, electroretinogram (ERG), fundus photography, and histological examination were used to assess the retinal function and structure. TUNEL, immunofluorescence and qPCR were used to evaluate cell apoptosis and expressions of Sirt1, Iba1, Bcl-2, Bax and Caspase-3. A significantly shortened latency of ERG waves was observed in ZYMT-treated mice, in comparison to those in the model group (P < 0.05). Histologically, ultrastructure of the retina was better preserved, and the outer nuclear layer (ONL) exhibited marked increase in thickness and cell count in ZYMP group (P < 0.05). The apoptosis rate was decreased markedly in ZYMT group. Immunofluorescence analysis showed that the expressions of Iba1 and Bcl-2 in the retina were increased, Bax and Caspase-3 were decreased after ZYMT intervention, while the qPCR revealed that the expressions of Iba1 and Sirt1 were significantly increased (P < 0.05). This study indicated that ZYMT has protective effect on retinal function and morphology of inherited RP mice in the early stage, possibly mediated via the regulation of antioxidant and anti-/pro-apoptotic factors expressions.http://www.sciencedirect.com/science/article/pii/S0753332223003682Retinitis pigmentosaChinese patent medicinesCell apoptosisMechanism |
| spellingShingle | Ziyang Huang Qiuling Huang Kai Xu Lina Liang Yamin Li Wei Zhou Na Ning Jiewen Zhou Jiajia Hu Shanshan Liu Lei Dang Protective effect of ZYMT, a traditional Chinese patent medicine in a mouse model of retinitis pigmentosa Biomedicine & Pharmacotherapy Retinitis pigmentosa Chinese patent medicines Cell apoptosis Mechanism |
| title | Protective effect of ZYMT, a traditional Chinese patent medicine in a mouse model of retinitis pigmentosa |
| title_full | Protective effect of ZYMT, a traditional Chinese patent medicine in a mouse model of retinitis pigmentosa |
| title_fullStr | Protective effect of ZYMT, a traditional Chinese patent medicine in a mouse model of retinitis pigmentosa |
| title_full_unstemmed | Protective effect of ZYMT, a traditional Chinese patent medicine in a mouse model of retinitis pigmentosa |
| title_short | Protective effect of ZYMT, a traditional Chinese patent medicine in a mouse model of retinitis pigmentosa |
| title_sort | protective effect of zymt a traditional chinese patent medicine in a mouse model of retinitis pigmentosa |
| topic | Retinitis pigmentosa Chinese patent medicines Cell apoptosis Mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223003682 |
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