A new enzymatic process for the treatment of phenolic pollutants

This work aimed to develop a new economic enzymatic process to treat the phenolic pollutants using pure tyrosinase in stirred vessel and adopting temperature (T), pH, rotational speed (N), initial phenol (C P,o) and enzyme (C T) concentrations as independent variables. Experimental data of the resid...

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Main Authors: Mauri Sergio Alves Palma, Harald Horn, Mario Zilli, Gisele Pigatto, Attilio Converti
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2013-08-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400016&lng=en&tlng=en
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author Mauri Sergio Alves Palma
Harald Horn
Mario Zilli
Gisele Pigatto
Attilio Converti
author_facet Mauri Sergio Alves Palma
Harald Horn
Mario Zilli
Gisele Pigatto
Attilio Converti
author_sort Mauri Sergio Alves Palma
collection DOAJ
description This work aimed to develop a new economic enzymatic process to treat the phenolic pollutants using pure tyrosinase in stirred vessel and adopting temperature (T), pH, rotational speed (N), initial phenol (C P,o) and enzyme (C T) concentrations as independent variables. Experimental data of the residual phenol concentration (C P) were used to calculate the oxidation efficiency (η), initial oxidation rate (-r o) and time required to reach the end of reaction (t) that were selected as the responses. Under the optimal conditions (T = 45°C, pH 6.6, N = 400 rpm, C P,o = 100 ppm and C T = 50 U/mL), η was 88.1%, -r o = 10.2 mg L-1 min-1, t = 40 min. These results suggested that tyrosinase-rich crude extracts from vegetable byproducts could be quite promising.
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spelling doaj.art-a111aa626cf045128cfb25b45e5e5c852022-12-22T03:18:55ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242013-08-0156465366210.1590/S1516-89132013000400016S1516-89132013000400016A new enzymatic process for the treatment of phenolic pollutantsMauri Sergio Alves Palma0Harald Horn1Mario Zilli2Gisele Pigatto3Attilio Converti4Universidade de São PauloTechnische Universität MünchenUniversità degli Studi di GenovaUniversidade de São PauloUniversità degli Studi di GenovaThis work aimed to develop a new economic enzymatic process to treat the phenolic pollutants using pure tyrosinase in stirred vessel and adopting temperature (T), pH, rotational speed (N), initial phenol (C P,o) and enzyme (C T) concentrations as independent variables. Experimental data of the residual phenol concentration (C P) were used to calculate the oxidation efficiency (η), initial oxidation rate (-r o) and time required to reach the end of reaction (t) that were selected as the responses. Under the optimal conditions (T = 45°C, pH 6.6, N = 400 rpm, C P,o = 100 ppm and C T = 50 U/mL), η was 88.1%, -r o = 10.2 mg L-1 min-1, t = 40 min. These results suggested that tyrosinase-rich crude extracts from vegetable byproducts could be quite promising.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400016&lng=en&tlng=enphenolstyrosinasewastesenzymatic treatment
spellingShingle Mauri Sergio Alves Palma
Harald Horn
Mario Zilli
Gisele Pigatto
Attilio Converti
A new enzymatic process for the treatment of phenolic pollutants
Brazilian Archives of Biology and Technology
phenols
tyrosinase
wastes
enzymatic treatment
title A new enzymatic process for the treatment of phenolic pollutants
title_full A new enzymatic process for the treatment of phenolic pollutants
title_fullStr A new enzymatic process for the treatment of phenolic pollutants
title_full_unstemmed A new enzymatic process for the treatment of phenolic pollutants
title_short A new enzymatic process for the treatment of phenolic pollutants
title_sort new enzymatic process for the treatment of phenolic pollutants
topic phenols
tyrosinase
wastes
enzymatic treatment
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400016&lng=en&tlng=en
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