Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms
Abstract Several oral diseases are characterized by a shift within the oral microbiome towards a pathogenic, dysbiotic composition. Broad-spectrum antimicrobials are often part of patient care. However, because of the rising antibiotic resistance, alternatives are increasingly desirable. Alternative...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-023-00370-y |
| _version_ | 1797946034168528896 |
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| author | Wannes Van Holm Rita Carvalho Lize Delanghe Tom Eilers Naiera Zayed Fabian Mermans Kristel Bernaerts Nico Boon Ingmar Claes Sarah Lebeer Wim Teughels |
| author_facet | Wannes Van Holm Rita Carvalho Lize Delanghe Tom Eilers Naiera Zayed Fabian Mermans Kristel Bernaerts Nico Boon Ingmar Claes Sarah Lebeer Wim Teughels |
| author_sort | Wannes Van Holm |
| collection | DOAJ |
| description | Abstract Several oral diseases are characterized by a shift within the oral microbiome towards a pathogenic, dysbiotic composition. Broad-spectrum antimicrobials are often part of patient care. However, because of the rising antibiotic resistance, alternatives are increasingly desirable. Alternatively, supplying beneficial species through probiotics is increasingly showing favorable results. Unfortunately, these probiotics are rarely evaluated comparatively. In this study, the in vitro effects of three known and three novel Lactobacillus strains, together with four novel Streptococcus salivarius strains were comparatively evaluated for antagonistic effects on proximal agar growth, antimicrobial properties of probiotic supernatant and the probiotic’s effects on in vitro periodontal biofilms. Strain-specific effects were observed as differences in efficacy between genera and differences within genera. While some of the Lactobacillus candidates were able to reduce the periodontal pathobiont A. actinomycetemcomitans, the S. salivarius strains were not. However, the S. salivarius strains were more effective against periodontal pathobionts P. intermedia, P. gingivalis, and F. nucleatum. Vexingly, most of the Lactobacillus strains also negatively affected the prevalence of commensal species within the biofilms, while this was lower for S. salivarius strains. Both within lactobacilli and streptococci, some strains showed significantly more inhibition of the pathobionts, indicating the importance of proper strain selection. Additionally, some species showed reductions in non-target species, which can result in unexpected and unexplored effects on the whole microbiome. |
| first_indexed | 2024-04-10T21:04:30Z |
| format | Article |
| id | doaj.art-a114db784f254f19af26d09c895de6d4 |
| institution | Directory Open Access Journal |
| issn | 2055-5008 |
| language | English |
| last_indexed | 2024-04-10T21:04:30Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj.art-a114db784f254f19af26d09c895de6d42023-01-22T12:06:07ZengNature Portfolionpj Biofilms and Microbiomes2055-50082023-01-019111210.1038/s41522-023-00370-yAntimicrobial potential of known and novel probiotics on in vitro periodontitis biofilmsWannes Van Holm0Rita Carvalho1Lize Delanghe2Tom Eilers3Naiera Zayed4Fabian Mermans5Kristel Bernaerts6Nico Boon7Ingmar Claes8Sarah Lebeer9Wim Teughels10Department of Oral Health Sciences, University of Leuven (KU Leuven)Department of Oral Health Sciences, University of Leuven (KU Leuven)Department of Bioscience Engineering, University of AntwerpDepartment of Bioscience Engineering, University of AntwerpDepartment of Oral Health Sciences, University of Leuven (KU Leuven)Centre for Microbial Ecology and Technology (CMET), Ghent University (UGent)Bio- and Chemical Systems Technology, Reactor Engineering and Safety, Department of Chemical Engineering, University of Leuven (KU Leuven)Centre for Microbial Ecology and Technology (CMET), Ghent University (UGent)YUN NVDepartment of Bioscience Engineering, University of AntwerpDepartment of Oral Health Sciences, University of Leuven (KU Leuven)Abstract Several oral diseases are characterized by a shift within the oral microbiome towards a pathogenic, dysbiotic composition. Broad-spectrum antimicrobials are often part of patient care. However, because of the rising antibiotic resistance, alternatives are increasingly desirable. Alternatively, supplying beneficial species through probiotics is increasingly showing favorable results. Unfortunately, these probiotics are rarely evaluated comparatively. In this study, the in vitro effects of three known and three novel Lactobacillus strains, together with four novel Streptococcus salivarius strains were comparatively evaluated for antagonistic effects on proximal agar growth, antimicrobial properties of probiotic supernatant and the probiotic’s effects on in vitro periodontal biofilms. Strain-specific effects were observed as differences in efficacy between genera and differences within genera. While some of the Lactobacillus candidates were able to reduce the periodontal pathobiont A. actinomycetemcomitans, the S. salivarius strains were not. However, the S. salivarius strains were more effective against periodontal pathobionts P. intermedia, P. gingivalis, and F. nucleatum. Vexingly, most of the Lactobacillus strains also negatively affected the prevalence of commensal species within the biofilms, while this was lower for S. salivarius strains. Both within lactobacilli and streptococci, some strains showed significantly more inhibition of the pathobionts, indicating the importance of proper strain selection. Additionally, some species showed reductions in non-target species, which can result in unexpected and unexplored effects on the whole microbiome.https://doi.org/10.1038/s41522-023-00370-y |
| spellingShingle | Wannes Van Holm Rita Carvalho Lize Delanghe Tom Eilers Naiera Zayed Fabian Mermans Kristel Bernaerts Nico Boon Ingmar Claes Sarah Lebeer Wim Teughels Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms npj Biofilms and Microbiomes |
| title | Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms |
| title_full | Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms |
| title_fullStr | Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms |
| title_full_unstemmed | Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms |
| title_short | Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms |
| title_sort | antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms |
| url | https://doi.org/10.1038/s41522-023-00370-y |
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