Application of bioelectric effect to reduce the antibiotic resistance of subgingival plaque biofilm: An in vitro study
Context: Biofilms are known for their antimicrobial resistance, and so is the subgingival plaque biofilm, the primary etiologic factor for periodontal infections. Aims: The objective of this study is to investigate if the subgingival plaque biofilm resistance can be reduced using doxycycline in the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Journal of Indian Society of Periodontology |
| Subjects: | |
| Online Access: | http://www.jisponline.com/article.asp?issn=0972-124X;year=2018;volume=22;issue=2;spage=133;epage=139;aulast=Hari |
| _version_ | 1819014895187787776 |
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| author | Padmini Hari Kranthi Raja Kacharaju Naveen Anumala Krishnanjaneya Reddy Pathakota Jayakumar Avula |
| author_facet | Padmini Hari Kranthi Raja Kacharaju Naveen Anumala Krishnanjaneya Reddy Pathakota Jayakumar Avula |
| author_sort | Padmini Hari |
| collection | DOAJ |
| description | Context: Biofilms are known for their antimicrobial resistance, and so is the subgingival plaque biofilm, the primary etiologic factor for periodontal infections. Aims: The objective of this study is to investigate if the subgingival plaque biofilm resistance can be reduced using doxycycline in the presence of low-intensity electric field (bioelectric effect). Settings and Design: The study was an in vitro microbiological study. Materials and Methods: Subgingival plaque samples from chronic periodontitis patients were collected to grow subgingival plaque biofilms on hydroxyapatite disks. Hydroxyapatite disks with the plaque biofilms from each patient were divided into four groups: (i) No intervention – control, (ii) current alone – CU; (iii) doxycycline – AB, and (iv) combined treatment – CU + AB. After respective treatments, the disks were anaerobically incubated for 48 h, the biofilm was dispersed and subcultured and colony-forming unit/mL was estimated in all the four groups. Statistical Analysis: Statistical analysis was done using Mann–Whitney and Kruskal–Wallis tests for intergroup comparisons. T-test was done to assess the difference in current flow between the groups CU and CU + AB. Results: All the three treatment modalities showed antibacterial effect. Application of current alone resulted in reduced bacterial growth than control group. Doxycycline alone resulted in reduction in bacterial counts better than control and current alone groups. The combination treatment showed greatest inhibition of bacterial colonies. Conclusion: The ability of doxycycline antibiotic in inhibiting plaque biofilm was significantly enhanced by application of a weak electric field (5 volts for 2 min). |
| first_indexed | 2024-12-21T02:23:06Z |
| format | Article |
| id | doaj.art-a117b505ba3f48a1a5cfe20737ef6324 |
| institution | Directory Open Access Journal |
| issn | 0972-124X |
| language | English |
| last_indexed | 2024-12-21T02:23:06Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Society of Periodontology |
| spelling | doaj.art-a117b505ba3f48a1a5cfe20737ef63242022-12-21T19:19:04ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X2018-01-0122213313910.4103/jisp.jisp_320_17Application of bioelectric effect to reduce the antibiotic resistance of subgingival plaque biofilm: An in vitro studyPadmini HariKranthi Raja KacharajuNaveen AnumalaKrishnanjaneya Reddy PathakotaJayakumar AvulaContext: Biofilms are known for their antimicrobial resistance, and so is the subgingival plaque biofilm, the primary etiologic factor for periodontal infections. Aims: The objective of this study is to investigate if the subgingival plaque biofilm resistance can be reduced using doxycycline in the presence of low-intensity electric field (bioelectric effect). Settings and Design: The study was an in vitro microbiological study. Materials and Methods: Subgingival plaque samples from chronic periodontitis patients were collected to grow subgingival plaque biofilms on hydroxyapatite disks. Hydroxyapatite disks with the plaque biofilms from each patient were divided into four groups: (i) No intervention – control, (ii) current alone – CU; (iii) doxycycline – AB, and (iv) combined treatment – CU + AB. After respective treatments, the disks were anaerobically incubated for 48 h, the biofilm was dispersed and subcultured and colony-forming unit/mL was estimated in all the four groups. Statistical Analysis: Statistical analysis was done using Mann–Whitney and Kruskal–Wallis tests for intergroup comparisons. T-test was done to assess the difference in current flow between the groups CU and CU + AB. Results: All the three treatment modalities showed antibacterial effect. Application of current alone resulted in reduced bacterial growth than control group. Doxycycline alone resulted in reduction in bacterial counts better than control and current alone groups. The combination treatment showed greatest inhibition of bacterial colonies. Conclusion: The ability of doxycycline antibiotic in inhibiting plaque biofilm was significantly enhanced by application of a weak electric field (5 volts for 2 min).http://www.jisponline.com/article.asp?issn=0972-124X;year=2018;volume=22;issue=2;spage=133;epage=139;aulast=HariAntimicrobial resistancebiofilmdental plaquedoxycyclineperiodontitis |
| spellingShingle | Padmini Hari Kranthi Raja Kacharaju Naveen Anumala Krishnanjaneya Reddy Pathakota Jayakumar Avula Application of bioelectric effect to reduce the antibiotic resistance of subgingival plaque biofilm: An in vitro study Journal of Indian Society of Periodontology Antimicrobial resistance biofilm dental plaque doxycycline periodontitis |
| title | Application of bioelectric effect to reduce the antibiotic resistance of subgingival plaque biofilm: An in vitro study |
| title_full | Application of bioelectric effect to reduce the antibiotic resistance of subgingival plaque biofilm: An in vitro study |
| title_fullStr | Application of bioelectric effect to reduce the antibiotic resistance of subgingival plaque biofilm: An in vitro study |
| title_full_unstemmed | Application of bioelectric effect to reduce the antibiotic resistance of subgingival plaque biofilm: An in vitro study |
| title_short | Application of bioelectric effect to reduce the antibiotic resistance of subgingival plaque biofilm: An in vitro study |
| title_sort | application of bioelectric effect to reduce the antibiotic resistance of subgingival plaque biofilm an in vitro study |
| topic | Antimicrobial resistance biofilm dental plaque doxycycline periodontitis |
| url | http://www.jisponline.com/article.asp?issn=0972-124X;year=2018;volume=22;issue=2;spage=133;epage=139;aulast=Hari |
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