Genetic diversity and population structure of ginseng in China based on RAPD analysis
Population genetic diversity was estimated from forty-four individual ginseng (Panax ginsengC.A. Meyer) plants collected from seven geographical populations located in Heilongjiang, Liaoning, and Jilin Provinces of China as well as the People’s Republic of Korea by using randomly amplified polymorph...
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| Format: | Article |
| Language: | English |
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De Gruyter
2016-01-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2016-0051 |
| _version_ | 1818730260543307776 |
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| author | Wang Shi-jie Chen Xiao-lin Han Feng-bo Li Ru-sheng Li Gang Zhao Yan Xu Yong-hua Zhang Lian-xue |
| author_facet | Wang Shi-jie Chen Xiao-lin Han Feng-bo Li Ru-sheng Li Gang Zhao Yan Xu Yong-hua Zhang Lian-xue |
| author_sort | Wang Shi-jie |
| collection | DOAJ |
| description | Population genetic diversity was estimated from forty-four individual ginseng (Panax ginsengC.A. Meyer) plants collected from seven geographical populations located in Heilongjiang, Liaoning, and Jilin Provinces of China as well as the People’s Republic of Korea by using randomly amplified polymorphic DNA (RAPD) markers. Overall, 41 polymorphic loci were amplified using ten primer pairs. The polymorphism percentage ranged from 50% to 100% among seven local populations of ginseng, indicating that there is plentiful genetic diversity in wild ginseng populations. The genetic diversity at the species level was higher than that at the population level. Variance analysis showed that there was a significant difference among populations in genetic diversity. The genetic differentiation coefficient (i.e., FST) indicates that 43% of the variation occurred among populations, which indicates that substantial genetic differentiation occurred among populations. At the same time, the measured value of gene flow (Nm) was 0.66 based on the observed genetic differentiation coefficient among populations, suggesting there was moderate gene flow among populations. |
| first_indexed | 2024-12-17T22:58:57Z |
| format | Article |
| id | doaj.art-a117bc883896423eb9bf904703f4815c |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-17T22:58:57Z |
| publishDate | 2016-01-01 |
| publisher | De Gruyter |
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| series | Open Life Sciences |
| spelling | doaj.art-a117bc883896423eb9bf904703f4815c2022-12-21T21:29:27ZengDe GruyterOpen Life Sciences2391-54122016-01-0111138739010.1515/biol-2016-0051biol-2016-0051Genetic diversity and population structure of ginseng in China based on RAPD analysisWang Shi-jieChen Xiao-lin0Han Feng-bo1Li Ru-sheng2Li Gang3Zhao Yan4Xu Yong-hua5Zhang Lian-xue6Jilin Ginseng & Pilose Antler Office, Changchun 130022, Jilin Province, P.R.ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural Science and Technology University, Jilin132101, Jilin Province, P.R.ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural Science and Technology University, Jilin132101, Jilin Province, P.R.ChinaJilin Academy of Agricultural Sciences, 130033, Jilin Province, P.R.ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, Jilin Province, P.R.ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, Jilin Province, P.R.ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, Jilin Province, P.R.ChinaPopulation genetic diversity was estimated from forty-four individual ginseng (Panax ginsengC.A. Meyer) plants collected from seven geographical populations located in Heilongjiang, Liaoning, and Jilin Provinces of China as well as the People’s Republic of Korea by using randomly amplified polymorphic DNA (RAPD) markers. Overall, 41 polymorphic loci were amplified using ten primer pairs. The polymorphism percentage ranged from 50% to 100% among seven local populations of ginseng, indicating that there is plentiful genetic diversity in wild ginseng populations. The genetic diversity at the species level was higher than that at the population level. Variance analysis showed that there was a significant difference among populations in genetic diversity. The genetic differentiation coefficient (i.e., FST) indicates that 43% of the variation occurred among populations, which indicates that substantial genetic differentiation occurred among populations. At the same time, the measured value of gene flow (Nm) was 0.66 based on the observed genetic differentiation coefficient among populations, suggesting there was moderate gene flow among populations.https://doi.org/10.1515/biol-2016-0051panax ginsengrapdgenetic diversitypopulation structure |
| spellingShingle | Wang Shi-jie Chen Xiao-lin Han Feng-bo Li Ru-sheng Li Gang Zhao Yan Xu Yong-hua Zhang Lian-xue Genetic diversity and population structure of ginseng in China based on RAPD analysis Open Life Sciences panax ginseng rapd genetic diversity population structure |
| title | Genetic diversity and population structure of ginseng in China based on RAPD analysis |
| title_full | Genetic diversity and population structure of ginseng in China based on RAPD analysis |
| title_fullStr | Genetic diversity and population structure of ginseng in China based on RAPD analysis |
| title_full_unstemmed | Genetic diversity and population structure of ginseng in China based on RAPD analysis |
| title_short | Genetic diversity and population structure of ginseng in China based on RAPD analysis |
| title_sort | genetic diversity and population structure of ginseng in china based on rapd analysis |
| topic | panax ginseng rapd genetic diversity population structure |
| url | https://doi.org/10.1515/biol-2016-0051 |
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