Application of heat-treated oyster shell powder to induce priming of Theobroma cocoa seedlings plant defense system against Phytophthora megakarya attack
Priming with natural compounds is an interesting alternative for sustainable agriculture to explore the molecular mechanisms associated with the management of diseases caused by soil borne pathogens. The aim of this work was to evaluate the effect of the soil amendment with bio-oyster shell on the s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Current Plant Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662823000129 |
| _version_ | 1797815723391713280 |
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| author | Tene Tayo Paul Martial Mouafo Tchinda Romaric Armel Yamoneka Juste Dzelamonyuy Aristide Wouamba Njonte Steven Collins Boudjeko Thaddee |
| author_facet | Tene Tayo Paul Martial Mouafo Tchinda Romaric Armel Yamoneka Juste Dzelamonyuy Aristide Wouamba Njonte Steven Collins Boudjeko Thaddee |
| author_sort | Tene Tayo Paul Martial |
| collection | DOAJ |
| description | Priming with natural compounds is an interesting alternative for sustainable agriculture to explore the molecular mechanisms associated with the management of diseases caused by soil borne pathogens. The aim of this work was to evaluate the effect of the soil amendment with bio-oyster shell on the stimulation of the cocoa plant defense systems against P. megakarya in the nursery. For this purpose, the potential of bio-oyster shell to stimulate cocoa plant defense mechanisms was evaluated by soil amendment at 1% W/W under greenhouse conditions. After soil amendment, agronomic growth parameters and soil parameters were analyzed. The degree of expression of phytoalexins and defense genes was carried out by UPLC-DAD- HRESI–MS (+) and qRT-PCR analyses, respectively. The results obtained show that soil treatment with these composites improves the sanitary quality of cocoa seedlings in controlled conditions through the stimulation of agronomic growth parameters such as plant height, number of leaves, leaf surface area, and fresh weight of shoot and root. The latter leads to an increase in soil pH by about 32 % as well as the improvement of the nutritional status of the soil by 90%. Moreover, bio-oyster shell soil amendment induces up-regulation of TcPer-1, TcGlu1, TcChiB, and TcMYBPA genes and accumulation of primed metabolites such as flavonols, brevifolin, and isoflavone compounds before and after infection. This stimulation based on biological substances make it possible to pre-immunize cocoa plants against pathogen attacks and would be a good candidate to improve the quality of cocoa seeds in Cameroon. |
| first_indexed | 2024-03-13T08:27:00Z |
| format | Article |
| id | doaj.art-a11ba73691e940dbbd1f1d27ee2032ec |
| institution | Directory Open Access Journal |
| issn | 2214-6628 |
| language | English |
| last_indexed | 2024-03-13T08:27:00Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj.art-a11ba73691e940dbbd1f1d27ee2032ec2023-05-31T04:44:24ZengElsevierCurrent Plant Biology2214-66282023-06-0134100283Application of heat-treated oyster shell powder to induce priming of Theobroma cocoa seedlings plant defense system against Phytophthora megakarya attackTene Tayo Paul Martial0Mouafo Tchinda Romaric Armel1Yamoneka Juste2Dzelamonyuy Aristide3Wouamba Njonte Steven Collins4Boudjeko Thaddee5Laboratory of Phytoprotection and Genetic Ressources Valorization, Biotechnology Center, University of Yaoundé I, P.O. Box 17673, Etetak, Yaoundé, Cameroon; Corresponding author.Plant Pathology Department, University of Florida, 2550 Hull Road, PO Box 110680, Gainesville, FL, USA; Food Systems Institute, University of Florida, 2550 Hull Road, PO Box 110680, Gainesville, FL, USA; Emerging Pathogens Institute, University of Florida, 2055 Mowry road, PO Box 100009, Gainesville, FL, USADepartment of Chemistry and Agricultural Industries, Agronomy Faculty, University of Goma, P.O BOX: 204, Goma, Democratic Republic of CongoDepartment of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, MS1017Q Lab, MS10115 Indianapolis, IN, USADepartment of Chemistry, Higher Teacher Training College, University of Yaounde 1, P.O BOX 47, Yaoundé, CameroonLaboratory of Phytoprotection and Genetic Ressources Valorization, Biotechnology Center, University of Yaoundé I, P.O. Box 17673, Etetak, Yaoundé, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaoundé, CameroonPriming with natural compounds is an interesting alternative for sustainable agriculture to explore the molecular mechanisms associated with the management of diseases caused by soil borne pathogens. The aim of this work was to evaluate the effect of the soil amendment with bio-oyster shell on the stimulation of the cocoa plant defense systems against P. megakarya in the nursery. For this purpose, the potential of bio-oyster shell to stimulate cocoa plant defense mechanisms was evaluated by soil amendment at 1% W/W under greenhouse conditions. After soil amendment, agronomic growth parameters and soil parameters were analyzed. The degree of expression of phytoalexins and defense genes was carried out by UPLC-DAD- HRESI–MS (+) and qRT-PCR analyses, respectively. The results obtained show that soil treatment with these composites improves the sanitary quality of cocoa seedlings in controlled conditions through the stimulation of agronomic growth parameters such as plant height, number of leaves, leaf surface area, and fresh weight of shoot and root. The latter leads to an increase in soil pH by about 32 % as well as the improvement of the nutritional status of the soil by 90%. Moreover, bio-oyster shell soil amendment induces up-regulation of TcPer-1, TcGlu1, TcChiB, and TcMYBPA genes and accumulation of primed metabolites such as flavonols, brevifolin, and isoflavone compounds before and after infection. This stimulation based on biological substances make it possible to pre-immunize cocoa plants against pathogen attacks and would be a good candidate to improve the quality of cocoa seeds in Cameroon.http://www.sciencedirect.com/science/article/pii/S2214662823000129Black pod diseaseBio-oyster shellCocoaPriming |
| spellingShingle | Tene Tayo Paul Martial Mouafo Tchinda Romaric Armel Yamoneka Juste Dzelamonyuy Aristide Wouamba Njonte Steven Collins Boudjeko Thaddee Application of heat-treated oyster shell powder to induce priming of Theobroma cocoa seedlings plant defense system against Phytophthora megakarya attack Current Plant Biology Black pod disease Bio-oyster shell Cocoa Priming |
| title | Application of heat-treated oyster shell powder to induce priming of Theobroma cocoa seedlings plant defense system against Phytophthora megakarya attack |
| title_full | Application of heat-treated oyster shell powder to induce priming of Theobroma cocoa seedlings plant defense system against Phytophthora megakarya attack |
| title_fullStr | Application of heat-treated oyster shell powder to induce priming of Theobroma cocoa seedlings plant defense system against Phytophthora megakarya attack |
| title_full_unstemmed | Application of heat-treated oyster shell powder to induce priming of Theobroma cocoa seedlings plant defense system against Phytophthora megakarya attack |
| title_short | Application of heat-treated oyster shell powder to induce priming of Theobroma cocoa seedlings plant defense system against Phytophthora megakarya attack |
| title_sort | application of heat treated oyster shell powder to induce priming of theobroma cocoa seedlings plant defense system against phytophthora megakarya attack |
| topic | Black pod disease Bio-oyster shell Cocoa Priming |
| url | http://www.sciencedirect.com/science/article/pii/S2214662823000129 |
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