A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria
Treatment options for multidrug-resistant bacterial infections are limited and often ineffective. Fecal microbiota transplantation (FMT) has emerged as a promising therapy for intestinal multidrug-resistant bacterial decolonization. However, clinical results are discrepant. The aim of our pilot stud...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/11/2753 |
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| author | Angélique Salandre Johanne Delannoy Marie Thérèse Barba Goudiaby Frédéric Barbut Muriel Thomas Anne-Judith Waligora-Dupriet Nathalie Kapel |
| author_facet | Angélique Salandre Johanne Delannoy Marie Thérèse Barba Goudiaby Frédéric Barbut Muriel Thomas Anne-Judith Waligora-Dupriet Nathalie Kapel |
| author_sort | Angélique Salandre |
| collection | DOAJ |
| description | Treatment options for multidrug-resistant bacterial infections are limited and often ineffective. Fecal microbiota transplantation (FMT) has emerged as a promising therapy for intestinal multidrug-resistant bacterial decolonization. However, clinical results are discrepant. The aim of our pilot study was to evaluate the screening performance of a simple diagnostic tool to select fecal samples that will be effective in decolonizing the intestine. Fecal samples from 10 healthy subjects were selected. We developed an agar spot test to evaluate their antagonistic activity toward the growth of VanA <i>Enterococcus faecium</i> and OXA-48-producing <i>Klebsiella pneumoniae</i>, two of the most serious and urgent threats of antibiotic resistance. Most fecal samples were able to limit the growth of both bacteria in vitro but with large inter-individual variation. The samples with the highest and lowest antagonistic activity were used for FMT in a mouse model of intestinal colonization. FMT was not successful in reducing intestinal colonization with VanA <i>Enterococcus faecium</i>, whereas FMT performed with the fecal sample showing the highest activity on the agar spot test was able to significantly reduce the intestinal colonization of mice with <i>Klebsiella pneumoniae</i> OXA-48. The agar spot test could thus serve as a reliable screening tool to select stool samples with the best potential to eradicate/reduce multidrug-resistant bacteria carriage after FMT. |
| first_indexed | 2024-03-09T16:35:28Z |
| format | Article |
| id | doaj.art-a123ae6520204306817958efac02d84c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:35:28Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a123ae6520204306817958efac02d84c2023-11-24T14:57:09ZengMDPI AGMicroorganisms2076-26072023-11-011111275310.3390/microorganisms11112753A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant BacteriaAngélique Salandre0Johanne Delannoy1Marie Thérèse Barba Goudiaby2Frédéric Barbut3Muriel Thomas4Anne-Judith Waligora-Dupriet5Nathalie Kapel6INSERM UMR-S1139, Faculty of Pharmacy, Université Paris Cité, F-75006 Paris, FranceINSERM UMR-S1139, Faculty of Pharmacy, Université Paris Cité, F-75006 Paris, FranceINSERM UMR-S1139, Faculty of Pharmacy, Université Paris Cité, F-75006 Paris, FranceINSERM UMR-S1139, Faculty of Pharmacy, Université Paris Cité, F-75006 Paris, FranceParis Center for Microbiome Medicine (PaCeMM), Fédération Hospitalo-Universitaire, F-75011 Paris, FranceINSERM UMR-S1139, Faculty of Pharmacy, Université Paris Cité, F-75006 Paris, FranceINSERM UMR-S1139, Faculty of Pharmacy, Université Paris Cité, F-75006 Paris, FranceTreatment options for multidrug-resistant bacterial infections are limited and often ineffective. Fecal microbiota transplantation (FMT) has emerged as a promising therapy for intestinal multidrug-resistant bacterial decolonization. However, clinical results are discrepant. The aim of our pilot study was to evaluate the screening performance of a simple diagnostic tool to select fecal samples that will be effective in decolonizing the intestine. Fecal samples from 10 healthy subjects were selected. We developed an agar spot test to evaluate their antagonistic activity toward the growth of VanA <i>Enterococcus faecium</i> and OXA-48-producing <i>Klebsiella pneumoniae</i>, two of the most serious and urgent threats of antibiotic resistance. Most fecal samples were able to limit the growth of both bacteria in vitro but with large inter-individual variation. The samples with the highest and lowest antagonistic activity were used for FMT in a mouse model of intestinal colonization. FMT was not successful in reducing intestinal colonization with VanA <i>Enterococcus faecium</i>, whereas FMT performed with the fecal sample showing the highest activity on the agar spot test was able to significantly reduce the intestinal colonization of mice with <i>Klebsiella pneumoniae</i> OXA-48. The agar spot test could thus serve as a reliable screening tool to select stool samples with the best potential to eradicate/reduce multidrug-resistant bacteria carriage after FMT.https://www.mdpi.com/2076-2607/11/11/2753fecal microbiota transplantXDR bacteria<i>Klebsiella pneumoniae</i> OXA-48<i>Enterococcus faecium</i> VanAscreening methodpre-clinical model |
| spellingShingle | Angélique Salandre Johanne Delannoy Marie Thérèse Barba Goudiaby Frédéric Barbut Muriel Thomas Anne-Judith Waligora-Dupriet Nathalie Kapel A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria Microorganisms fecal microbiota transplant XDR bacteria <i>Klebsiella pneumoniae</i> OXA-48 <i>Enterococcus faecium</i> VanA screening method pre-clinical model |
| title | A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria |
| title_full | A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria |
| title_fullStr | A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria |
| title_full_unstemmed | A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria |
| title_short | A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria |
| title_sort | simple in vitro test to select stools for fecal microbiota transplantation to limit intestinal carriage of extensively drug resistant bacteria |
| topic | fecal microbiota transplant XDR bacteria <i>Klebsiella pneumoniae</i> OXA-48 <i>Enterococcus faecium</i> VanA screening method pre-clinical model |
| url | https://www.mdpi.com/2076-2607/11/11/2753 |
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