An Experimental Swallow Evoked Potential Protocol to Investigate the Neural Substrates of Swallowing
Advancement in dysphagia intervention is hindered by our lack of understanding of the neural mechanisms of swallowing in health and disease. Evoking and understanding neural activity in response to normal and disordered swallowing is essential to bridge this knowledge gap. Building on sensory evoked...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-03-01
|
| Series: | OTO Open |
| Online Access: | https://doi.org/10.1177/2473974X20913542 |
| _version_ | 1797641422630813696 |
|---|---|
| author | Ashley Kloepper MPH Joseph Arnold Alexis Ruffolo MD Brian Kinealy Chandler Haxton Nicole Nichols PhD Kazutaka Takahashi PhD Teresa E. Lever PhD |
| author_facet | Ashley Kloepper MPH Joseph Arnold Alexis Ruffolo MD Brian Kinealy Chandler Haxton Nicole Nichols PhD Kazutaka Takahashi PhD Teresa E. Lever PhD |
| author_sort | Ashley Kloepper MPH |
| collection | DOAJ |
| description | Advancement in dysphagia intervention is hindered by our lack of understanding of the neural mechanisms of swallowing in health and disease. Evoking and understanding neural activity in response to normal and disordered swallowing is essential to bridge this knowledge gap. Building on sensory evoked potential methodology, we developed a minimally invasive approach to generate swallow evoked potentials (SwEPs) in response to repetitive swallowing induced by citric acid stimulation of the oropharynx in lightly anesthetized healthy adult rats. The SwEP waveform consisted of 8 replicable peaks within 10 milliseconds immediately preceding the onset of electromyographic swallowing activity. Methodology refinement is underway with healthy rats to establish normative SwEP waveform morphology before proceeding to models of advanced aging and age-related neurodegenerative diseases. Ultimately, we envision that this experimental protocol may unmask the pathologic neural substrates contributing to dysphagia to accelerate the discovery of targeted therapeutics. |
| first_indexed | 2024-03-11T13:44:25Z |
| format | Article |
| id | doaj.art-a1266609d0274ab2ae743b5c5d4e97c3 |
| institution | Directory Open Access Journal |
| issn | 2473-974X |
| language | English |
| last_indexed | 2024-03-11T13:44:25Z |
| publishDate | 2020-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | OTO Open |
| spelling | doaj.art-a1266609d0274ab2ae743b5c5d4e97c32023-11-02T10:49:41ZengWileyOTO Open2473-974X2020-03-01410.1177/2473974X20913542An Experimental Swallow Evoked Potential Protocol to Investigate the Neural Substrates of SwallowingAshley Kloepper MPH0Joseph Arnold1Alexis Ruffolo MD2Brian Kinealy3Chandler Haxton4Nicole Nichols PhD5Kazutaka Takahashi PhD6Teresa E. Lever PhD7Department of Otolaryngology–Head and Neck Surgery, School of Medicine, University of Missouri, Columbia, Missouri, USADepartment of Otolaryngology–Head and Neck Surgery, School of Medicine, University of Missouri, Columbia, Missouri, USADepartment of Otolaryngology–Head and Neck Surgery, School of Medicine, University of Missouri, Columbia, Missouri, USADepartment of Otolaryngology–Head and Neck Surgery, School of Medicine, University of Missouri, Columbia, Missouri, USADepartment of Otolaryngology–Head and Neck Surgery, School of Medicine, University of Missouri, Columbia, Missouri, USADepartment of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USAResearch Computing Center, University of Chicago, Chicago, Illinois, USADepartment of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USAAdvancement in dysphagia intervention is hindered by our lack of understanding of the neural mechanisms of swallowing in health and disease. Evoking and understanding neural activity in response to normal and disordered swallowing is essential to bridge this knowledge gap. Building on sensory evoked potential methodology, we developed a minimally invasive approach to generate swallow evoked potentials (SwEPs) in response to repetitive swallowing induced by citric acid stimulation of the oropharynx in lightly anesthetized healthy adult rats. The SwEP waveform consisted of 8 replicable peaks within 10 milliseconds immediately preceding the onset of electromyographic swallowing activity. Methodology refinement is underway with healthy rats to establish normative SwEP waveform morphology before proceeding to models of advanced aging and age-related neurodegenerative diseases. Ultimately, we envision that this experimental protocol may unmask the pathologic neural substrates contributing to dysphagia to accelerate the discovery of targeted therapeutics.https://doi.org/10.1177/2473974X20913542 |
| spellingShingle | Ashley Kloepper MPH Joseph Arnold Alexis Ruffolo MD Brian Kinealy Chandler Haxton Nicole Nichols PhD Kazutaka Takahashi PhD Teresa E. Lever PhD An Experimental Swallow Evoked Potential Protocol to Investigate the Neural Substrates of Swallowing OTO Open |
| title | An Experimental Swallow Evoked Potential Protocol to Investigate the Neural Substrates of Swallowing |
| title_full | An Experimental Swallow Evoked Potential Protocol to Investigate the Neural Substrates of Swallowing |
| title_fullStr | An Experimental Swallow Evoked Potential Protocol to Investigate the Neural Substrates of Swallowing |
| title_full_unstemmed | An Experimental Swallow Evoked Potential Protocol to Investigate the Neural Substrates of Swallowing |
| title_short | An Experimental Swallow Evoked Potential Protocol to Investigate the Neural Substrates of Swallowing |
| title_sort | experimental swallow evoked potential protocol to investigate the neural substrates of swallowing |
| url | https://doi.org/10.1177/2473974X20913542 |
| work_keys_str_mv | AT ashleykloeppermph anexperimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT josepharnold anexperimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT alexisruffolomd anexperimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT briankinealy anexperimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT chandlerhaxton anexperimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT nicolenicholsphd anexperimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT kazutakatakahashiphd anexperimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT teresaeleverphd anexperimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT ashleykloeppermph experimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT josepharnold experimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT alexisruffolomd experimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT briankinealy experimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT chandlerhaxton experimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT nicolenicholsphd experimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT kazutakatakahashiphd experimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing AT teresaeleverphd experimentalswallowevokedpotentialprotocoltoinvestigatetheneuralsubstratesofswallowing |