Protein Encapsulation: A Nanocarrier Approach to the Fluorescence Imaging of an Enzyme-Based Biomarker
Here, we report a new pentafluoropropanamido rhodamine fluorescent probe (ACS-HNE) that allows for the selective detection of neutrophil elastase (NE). ACS-HNE displayed high sensitivity, with a low limit of detection (<5.3 nM), and excellent selectivity toward elastase over other relevant bi...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-06-01
|
| Series: | Frontiers in Chemistry |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fchem.2020.00389/full |
| _version_ | 1818154881249181696 |
|---|---|
| author | Zhiyuan Jia Hai-Hao Han Adam C. Sedgwick George T. Williams Lauren Gwynne James T. Brewster Steven D. Bull A. Toby A. Jenkins Xiao-Peng He Holger Schönherr Jonathan L. Sessler Tony D. James |
| author_facet | Zhiyuan Jia Hai-Hao Han Adam C. Sedgwick George T. Williams Lauren Gwynne James T. Brewster Steven D. Bull A. Toby A. Jenkins Xiao-Peng He Holger Schönherr Jonathan L. Sessler Tony D. James |
| author_sort | Zhiyuan Jia |
| collection | DOAJ |
| description | Here, we report a new pentafluoropropanamido rhodamine fluorescent probe (ACS-HNE) that allows for the selective detection of neutrophil elastase (NE). ACS-HNE displayed high sensitivity, with a low limit of detection (<5.3 nM), and excellent selectivity toward elastase over other relevant biological analytes and enzymes. The comparatively poor solubility and cell permeability of neat ACS-HNE was improved by creating an ACS-HNE-albumin complex; this approach allowed for improvements in the in situ visualization of elastase activity in RAW 264.7 cells relative to ACS-HNE alone. The present study thus serves to demonstrate a simple universal strategy that may be used to overcome cell impermeability and solubility limitations, and to prepare probes suitable for the cellular imaging of enzymatic activity in vitro. |
| first_indexed | 2024-12-11T14:33:33Z |
| format | Article |
| id | doaj.art-a1278cfa6415454d85fd337a0bb33517 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-11T14:33:33Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-a1278cfa6415454d85fd337a0bb335172022-12-22T01:02:18ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462020-06-01810.3389/fchem.2020.00389537852Protein Encapsulation: A Nanocarrier Approach to the Fluorescence Imaging of an Enzyme-Based BiomarkerZhiyuan Jia0Hai-Hao Han1Adam C. Sedgwick2George T. Williams3Lauren Gwynne4James T. Brewster5Steven D. Bull6A. Toby A. Jenkins7Xiao-Peng He8Holger Schönherr9Jonathan L. Sessler10Tony D. James11Department of Chemistry and Biology, Physical Chemistry & Research Center of Micro- and Nanochemistry and Engineering (Cμ), University of Siegen, Siegen, GermanyKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, ChinaDepartment of Chemistry, The University of Texas at Austin, Austin, TX, United StatesDepartment of Chemistry, University of Bath, Bath, United KingdomDepartment of Chemistry, University of Bath, Bath, United KingdomDepartment of Chemistry, The University of Texas at Austin, Austin, TX, United StatesDepartment of Chemistry, University of Bath, Bath, United KingdomDepartment of Chemistry, University of Bath, Bath, United KingdomKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, ChinaDepartment of Chemistry and Biology, Physical Chemistry & Research Center of Micro- and Nanochemistry and Engineering (Cμ), University of Siegen, Siegen, GermanyDepartment of Chemistry, The University of Texas at Austin, Austin, TX, United StatesDepartment of Chemistry, University of Bath, Bath, United KingdomHere, we report a new pentafluoropropanamido rhodamine fluorescent probe (ACS-HNE) that allows for the selective detection of neutrophil elastase (NE). ACS-HNE displayed high sensitivity, with a low limit of detection (<5.3 nM), and excellent selectivity toward elastase over other relevant biological analytes and enzymes. The comparatively poor solubility and cell permeability of neat ACS-HNE was improved by creating an ACS-HNE-albumin complex; this approach allowed for improvements in the in situ visualization of elastase activity in RAW 264.7 cells relative to ACS-HNE alone. The present study thus serves to demonstrate a simple universal strategy that may be used to overcome cell impermeability and solubility limitations, and to prepare probes suitable for the cellular imaging of enzymatic activity in vitro.https://www.frontiersin.org/article/10.3389/fchem.2020.00389/fullelastase detectionBSA-based nanocarriernanocarrier-based enzyme detectionfluorescence imagingcell imaging |
| spellingShingle | Zhiyuan Jia Hai-Hao Han Adam C. Sedgwick George T. Williams Lauren Gwynne James T. Brewster Steven D. Bull A. Toby A. Jenkins Xiao-Peng He Holger Schönherr Jonathan L. Sessler Tony D. James Protein Encapsulation: A Nanocarrier Approach to the Fluorescence Imaging of an Enzyme-Based Biomarker Frontiers in Chemistry elastase detection BSA-based nanocarrier nanocarrier-based enzyme detection fluorescence imaging cell imaging |
| title | Protein Encapsulation: A Nanocarrier Approach to the Fluorescence Imaging of an Enzyme-Based Biomarker |
| title_full | Protein Encapsulation: A Nanocarrier Approach to the Fluorescence Imaging of an Enzyme-Based Biomarker |
| title_fullStr | Protein Encapsulation: A Nanocarrier Approach to the Fluorescence Imaging of an Enzyme-Based Biomarker |
| title_full_unstemmed | Protein Encapsulation: A Nanocarrier Approach to the Fluorescence Imaging of an Enzyme-Based Biomarker |
| title_short | Protein Encapsulation: A Nanocarrier Approach to the Fluorescence Imaging of an Enzyme-Based Biomarker |
| title_sort | protein encapsulation a nanocarrier approach to the fluorescence imaging of an enzyme based biomarker |
| topic | elastase detection BSA-based nanocarrier nanocarrier-based enzyme detection fluorescence imaging cell imaging |
| url | https://www.frontiersin.org/article/10.3389/fchem.2020.00389/full |
| work_keys_str_mv | AT zhiyuanjia proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT haihaohan proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT adamcsedgwick proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT georgetwilliams proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT laurengwynne proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT jamestbrewster proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT stevendbull proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT atobyajenkins proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT xiaopenghe proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT holgerschonherr proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT jonathanlsessler proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker AT tonydjames proteinencapsulationananocarrierapproachtothefluorescenceimagingofanenzymebasedbiomarker |