Topographical and Ultrastructural Evaluation of Titanium Plates Coated with PLGA, Chitosan, and/or Meropenem: An In Vitro Study
The present investigation was undertaken to evaluate the topographical and ultrastructural architecture of titanium plates coated with polylactic co-glycolic acid (PLGA), chitosan (CH), and/or meropenem (MEM) with or without <i>Staphylococcus aureus</i> (<i>SA</i>) or <i&g...
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MDPI AG
2022-11-01
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| Series: | Dentistry Journal |
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| Online Access: | https://www.mdpi.com/2304-6767/10/12/220 |
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| author | Mohammad Al-Qubaisey Rita Khounganian Abdulhakim Al-Badah Raisuddin Ali |
| author_facet | Mohammad Al-Qubaisey Rita Khounganian Abdulhakim Al-Badah Raisuddin Ali |
| author_sort | Mohammad Al-Qubaisey |
| collection | DOAJ |
| description | The present investigation was undertaken to evaluate the topographical and ultrastructural architecture of titanium plates coated with polylactic co-glycolic acid (PLGA), chitosan (CH), and/or meropenem (MEM) with or without <i>Staphylococcus aureus</i> (<i>SA</i>) or <i>Pseudomonas aeruginosa</i> (<i>PA</i>) bacteria. Single-hole segments of 0.4 mm thick, low-profile titanium plates were spray coated using an airbrush with polymeric carriers (PLGA or CH) loaded with MEM, in addition to the negative control group (uncoated titanium plates). The coated plates and the negative control group were subjected to bacterial biofilms through a cultivation process while being slowly stirred at 20 rpm for 24 h. The samples were fixed and processed for scanning electron microscopic study at 5, 10, and 20 k magnification. The data were statistically analyzed to compare within and between the different materials. Coating titanium plates with PLGA or CH with MEM appeared to enhance bacterial inhibition over uncoated plates, hindering biofilm formation and preventing bacterial proliferation. In the staphylococcus aureus group, the highest bacterial count was observed in the uncoated plates, whereas the lowest count was detected in meropenem-PLGA, followed by PLGA, chitosan, meropenem, and meropenem-chitosan, respectively. On the other hand, the <i>Pseudomonas aeruginosa</i> group with the uncoated plates had the highest bacterial count, whereas the lowest bacterial count was found related to CH, followed by PLGA, MP, MC, and MEM, respectively. |
| first_indexed | 2024-03-09T17:09:34Z |
| format | Article |
| id | doaj.art-a127ce1e77cb481696e6ebdf4bd63231 |
| institution | Directory Open Access Journal |
| issn | 2304-6767 |
| language | English |
| last_indexed | 2024-03-09T17:09:34Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj.art-a127ce1e77cb481696e6ebdf4bd632312023-11-24T14:14:04ZengMDPI AGDentistry Journal2304-67672022-11-01101222010.3390/dj10120220Topographical and Ultrastructural Evaluation of Titanium Plates Coated with PLGA, Chitosan, and/or Meropenem: An In Vitro StudyMohammad Al-Qubaisey0Rita Khounganian1Abdulhakim Al-Badah2Raisuddin Ali3Department of Dentistry, Riyadh 2nd Health Cluster, P.O. Box 60169, Riyadh 11545, Saudi ArabiaDepartment of Oral Medicine and Diagnostic Sciences, College of Dentistry, King Saud University, P.O. Box 60169, Riyadh 11545, Saudi ArabiaMicrobiology Laboratory, College of Dentistry, King Saud University, P.O. Box 60169, Riyadh 11545, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 60169, Riyadh 11545, Saudi ArabiaThe present investigation was undertaken to evaluate the topographical and ultrastructural architecture of titanium plates coated with polylactic co-glycolic acid (PLGA), chitosan (CH), and/or meropenem (MEM) with or without <i>Staphylococcus aureus</i> (<i>SA</i>) or <i>Pseudomonas aeruginosa</i> (<i>PA</i>) bacteria. Single-hole segments of 0.4 mm thick, low-profile titanium plates were spray coated using an airbrush with polymeric carriers (PLGA or CH) loaded with MEM, in addition to the negative control group (uncoated titanium plates). The coated plates and the negative control group were subjected to bacterial biofilms through a cultivation process while being slowly stirred at 20 rpm for 24 h. The samples were fixed and processed for scanning electron microscopic study at 5, 10, and 20 k magnification. The data were statistically analyzed to compare within and between the different materials. Coating titanium plates with PLGA or CH with MEM appeared to enhance bacterial inhibition over uncoated plates, hindering biofilm formation and preventing bacterial proliferation. In the staphylococcus aureus group, the highest bacterial count was observed in the uncoated plates, whereas the lowest count was detected in meropenem-PLGA, followed by PLGA, chitosan, meropenem, and meropenem-chitosan, respectively. On the other hand, the <i>Pseudomonas aeruginosa</i> group with the uncoated plates had the highest bacterial count, whereas the lowest bacterial count was found related to CH, followed by PLGA, MP, MC, and MEM, respectively.https://www.mdpi.com/2304-6767/10/12/220titanium platesscanning electron microscopeultrastructural topographychitosanPLGAmeropenem |
| spellingShingle | Mohammad Al-Qubaisey Rita Khounganian Abdulhakim Al-Badah Raisuddin Ali Topographical and Ultrastructural Evaluation of Titanium Plates Coated with PLGA, Chitosan, and/or Meropenem: An In Vitro Study Dentistry Journal titanium plates scanning electron microscope ultrastructural topography chitosan PLGA meropenem |
| title | Topographical and Ultrastructural Evaluation of Titanium Plates Coated with PLGA, Chitosan, and/or Meropenem: An In Vitro Study |
| title_full | Topographical and Ultrastructural Evaluation of Titanium Plates Coated with PLGA, Chitosan, and/or Meropenem: An In Vitro Study |
| title_fullStr | Topographical and Ultrastructural Evaluation of Titanium Plates Coated with PLGA, Chitosan, and/or Meropenem: An In Vitro Study |
| title_full_unstemmed | Topographical and Ultrastructural Evaluation of Titanium Plates Coated with PLGA, Chitosan, and/or Meropenem: An In Vitro Study |
| title_short | Topographical and Ultrastructural Evaluation of Titanium Plates Coated with PLGA, Chitosan, and/or Meropenem: An In Vitro Study |
| title_sort | topographical and ultrastructural evaluation of titanium plates coated with plga chitosan and or meropenem an in vitro study |
| topic | titanium plates scanning electron microscope ultrastructural topography chitosan PLGA meropenem |
| url | https://www.mdpi.com/2304-6767/10/12/220 |
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