Neuropharmacological effects of standardized aqueous stem bark extract of Parkia biglobossa in Wistar rats
Objective: Parkia biglobossa stem bark decoction is a popular medicinal plant preparation used as calming agent for tensed patients in traditional medicine. The aim of this study was to evaluate the effects of aqueous stem bark extract of Parkia biglobossa (AEPB) and its active fraction AEPBF3 on an...
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| Format: | Article |
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Mashhad University of Medical Sciences
2014-01-01
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| Series: | Avicenna Journal of Phytomedicine |
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| Online Access: | http://ajp.mums.ac.ir/pdf_1519_5dcfd93742add497bac2da4be59ec0d0.html |
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| author | Tijani Adeniyi Yahaya Samuel Ehiabhi Okhale Salawu Oluwakanyinsola Adeola |
| author_facet | Tijani Adeniyi Yahaya Samuel Ehiabhi Okhale Salawu Oluwakanyinsola Adeola |
| author_sort | Tijani Adeniyi Yahaya |
| collection | DOAJ |
| description | Objective: Parkia biglobossa stem bark decoction is a popular medicinal plant preparation used as calming agent for tensed patients in traditional medicine. The aim of this study was to evaluate the effects of aqueous stem bark extract of Parkia biglobossa (AEPB) and its active fraction AEPBF3 on anxiety, spontaneous alternation behavior, and locomotor activity. The open field apparatus was used to evaluate effects of AEPB and AEPBF3 on locomotion.The APBE and the active fraction AEPBF3 were standardized using reverse phase high performance liquid chromatography to establish finger print to ascertain identity and stability of the extracts over time. Materials and Methods: The oral median lethal doses (LD50) of AEPB and AEPBF3 were evaluated using modified Lorke’s method in rats. The effect of APBE (50-200 mg/kg p.o.), APBEF3 (25 and 50 mg/kg p.o.), diazepam (2.5 mg/kg, i.p.), and 10 ml normal saline/kg on anxiety-like behavior, spontaneous alternation behavior, and locomotion activity were evaluated in rats on elevated plus maze (EPM), Zero-maze, Y-maze, and open field apparatus, respectively. The oral LD50 values of AEPB and AEPBF3 were estimated to be 5000 mg/kg and 3800 mg/kg body weight in rats, respectively. Results: AEPB and AEPBF3 significantly (F6, 41=2342, p |
| first_indexed | 2024-12-10T10:41:01Z |
| format | Article |
| id | doaj.art-a1283cf3eaa043c0a42112b2c7d0880a |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-10T10:41:01Z |
| publishDate | 2014-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-a1283cf3eaa043c0a42112b2c7d0880a2022-12-22T01:52:19ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492014-01-014159711519Neuropharmacological effects of standardized aqueous stem bark extract of Parkia biglobossa in Wistar ratsTijani Adeniyi Yahaya0Samuel Ehiabhi Okhale1Salawu Oluwakanyinsola Adeola2Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development, P.M.B. 21, Garki -Abuja, NigeriaDepartment of Medicinal Plant Research and Traditional Medicine, National Institute for Pharmaceutical Research and Development, P.M.B. 21, Garki -Abuja, NigeriaDepartment of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development, P.M.B. 21, Garki -Abuja, NigeriaObjective: Parkia biglobossa stem bark decoction is a popular medicinal plant preparation used as calming agent for tensed patients in traditional medicine. The aim of this study was to evaluate the effects of aqueous stem bark extract of Parkia biglobossa (AEPB) and its active fraction AEPBF3 on anxiety, spontaneous alternation behavior, and locomotor activity. The open field apparatus was used to evaluate effects of AEPB and AEPBF3 on locomotion.The APBE and the active fraction AEPBF3 were standardized using reverse phase high performance liquid chromatography to establish finger print to ascertain identity and stability of the extracts over time. Materials and Methods: The oral median lethal doses (LD50) of AEPB and AEPBF3 were evaluated using modified Lorke’s method in rats. The effect of APBE (50-200 mg/kg p.o.), APBEF3 (25 and 50 mg/kg p.o.), diazepam (2.5 mg/kg, i.p.), and 10 ml normal saline/kg on anxiety-like behavior, spontaneous alternation behavior, and locomotion activity were evaluated in rats on elevated plus maze (EPM), Zero-maze, Y-maze, and open field apparatus, respectively. The oral LD50 values of AEPB and AEPBF3 were estimated to be 5000 mg/kg and 3800 mg/kg body weight in rats, respectively. Results: AEPB and AEPBF3 significantly (F6, 41=2342, phttp://ajp.mums.ac.ir/pdf_1519_5dcfd93742add497bac2da4be59ec0d0.htmlAnxietyLocomotionMemoryParkia biglobossaSpontaneous alternation behaviourY-maze |
| spellingShingle | Tijani Adeniyi Yahaya Samuel Ehiabhi Okhale Salawu Oluwakanyinsola Adeola Neuropharmacological effects of standardized aqueous stem bark extract of Parkia biglobossa in Wistar rats Avicenna Journal of Phytomedicine Anxiety Locomotion Memory Parkia biglobossa Spontaneous alternation behaviour Y-maze |
| title | Neuropharmacological effects of standardized aqueous stem bark extract of Parkia biglobossa in Wistar rats |
| title_full | Neuropharmacological effects of standardized aqueous stem bark extract of Parkia biglobossa in Wistar rats |
| title_fullStr | Neuropharmacological effects of standardized aqueous stem bark extract of Parkia biglobossa in Wistar rats |
| title_full_unstemmed | Neuropharmacological effects of standardized aqueous stem bark extract of Parkia biglobossa in Wistar rats |
| title_short | Neuropharmacological effects of standardized aqueous stem bark extract of Parkia biglobossa in Wistar rats |
| title_sort | neuropharmacological effects of standardized aqueous stem bark extract of parkia biglobossa in wistar rats |
| topic | Anxiety Locomotion Memory Parkia biglobossa Spontaneous alternation behaviour Y-maze |
| url | http://ajp.mums.ac.ir/pdf_1519_5dcfd93742add497bac2da4be59ec0d0.html |
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