Synovium-Derived Mesenchymal Stem Cells in Combination with Low Molecular Weight Hyaluronic Acid for Cartilage Repair
Regeneration of damaged articular cartilage remains one of the most complex and unresolved problems in traumatology and orthopedics. In this study, we investigated whether intra-articular injection of synovium-derived mesenchymal stem cells (SD-MSCs) with low molecular weight hyaluronic acid (LMWHA)...
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2022-12-01
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| Series: | International Journal of Biomedicine |
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| Online Access: | http://www.ijbm.org/articles/i48/ijbm_12(4)_oa4.pdf |
| _version_ | 1811178466553364480 |
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| author | Madina Sarsenova Ainur Mukhambetova Bakhtiyar Saginov Yerik Raimagambetov Vyacheslav Ogay |
| author_facet | Madina Sarsenova Ainur Mukhambetova Bakhtiyar Saginov Yerik Raimagambetov Vyacheslav Ogay |
| author_sort | Madina Sarsenova |
| collection | DOAJ |
| description | Regeneration of damaged articular cartilage remains one of the most complex and unresolved problems in traumatology and orthopedics. In this study, we investigated whether intra-articular injection of synovium-derived mesenchymal stem cells (SD-MSCs) with low molecular weight hyaluronic acid (LMWHA) could promote the regeneration of damaged cartilage in rabbits. To answer this question, rabbits' SD-MSCs were harvested, expanded in culture, and characterized by CFU assay and a multilineage differentiation test. For in vivo study, we created a defect within the cartilage layer without destroying subchondral bone. Two weeks after the cartilage defect, SD-MSCs (2×106 cells) were suspended in 0.5% LMWHA and injected into the left knee, and hyaluronic acid (HA) solution alone was placed into the right knee. Cartilage regeneration in experimental and control groups was evaluated macroscopically and histologically at Days 30, 60, and 90. The results of the study showed an early process of cartilage regeneration in the defect area on Day 30 after intra-articular MSCs-HA injection. Histological studies revealed that cartilage defect was covered by a thin layer of spindle-shaped undifferentiated cells and proliferated chondroblasts, in contrast to a single HA injection, which did not induce cartilage regeneration. On Day 60, we observed that the size of the cartilage defect after MSCs-HA injection significantly decreased, compared to one after HA injection. On Day 90, the cartilage defect in a knee treated with MSCs-HA was fully regenerated and was similar to intact cartilage. Thus, the combined application of the MSCs, HA, and chondroinductive proteins have a high therapeutic effect on cartilage defect regeneration in rabbits. |
| first_indexed | 2024-04-11T06:19:54Z |
| format | Article |
| id | doaj.art-a12908596cf74032be638776179898f9 |
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| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-04-11T06:19:54Z |
| publishDate | 2022-12-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj.art-a12908596cf74032be638776179898f92022-12-22T04:40:51ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292022-12-0112454855310.21103/Article12(4)_OA4Synovium-Derived Mesenchymal Stem Cells in Combination with Low Molecular Weight Hyaluronic Acid for Cartilage RepairMadina Sarsenova0Ainur Mukhambetova1Bakhtiyar Saginov2Yerik Raimagambetov3Vyacheslav Ogay4Stem Cell Laboratory, National Center for Biotechnology, Astana, Kazakhstan; School of Medicine, Nazarbayev University, Astana, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Astana, KazakhstanNational Scientific Center of Traumatology and Orthopedics named after academician N. D. Batpenov, Astana, KazakhstanNational Scientific Center of Traumatology and Orthopedics named after academician N. D. Batpenov, Astana, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Astana, KazakhstanRegeneration of damaged articular cartilage remains one of the most complex and unresolved problems in traumatology and orthopedics. In this study, we investigated whether intra-articular injection of synovium-derived mesenchymal stem cells (SD-MSCs) with low molecular weight hyaluronic acid (LMWHA) could promote the regeneration of damaged cartilage in rabbits. To answer this question, rabbits' SD-MSCs were harvested, expanded in culture, and characterized by CFU assay and a multilineage differentiation test. For in vivo study, we created a defect within the cartilage layer without destroying subchondral bone. Two weeks after the cartilage defect, SD-MSCs (2×106 cells) were suspended in 0.5% LMWHA and injected into the left knee, and hyaluronic acid (HA) solution alone was placed into the right knee. Cartilage regeneration in experimental and control groups was evaluated macroscopically and histologically at Days 30, 60, and 90. The results of the study showed an early process of cartilage regeneration in the defect area on Day 30 after intra-articular MSCs-HA injection. Histological studies revealed that cartilage defect was covered by a thin layer of spindle-shaped undifferentiated cells and proliferated chondroblasts, in contrast to a single HA injection, which did not induce cartilage regeneration. On Day 60, we observed that the size of the cartilage defect after MSCs-HA injection significantly decreased, compared to one after HA injection. On Day 90, the cartilage defect in a knee treated with MSCs-HA was fully regenerated and was similar to intact cartilage. Thus, the combined application of the MSCs, HA, and chondroinductive proteins have a high therapeutic effect on cartilage defect regeneration in rabbits.http://www.ijbm.org/articles/i48/ijbm_12(4)_oa4.pdfmesenchymal stem cellshyaluronic acidgrowth factorscartilage defectregenerationcell therapy |
| spellingShingle | Madina Sarsenova Ainur Mukhambetova Bakhtiyar Saginov Yerik Raimagambetov Vyacheslav Ogay Synovium-Derived Mesenchymal Stem Cells in Combination with Low Molecular Weight Hyaluronic Acid for Cartilage Repair International Journal of Biomedicine mesenchymal stem cells hyaluronic acid growth factors cartilage defect regeneration cell therapy |
| title | Synovium-Derived Mesenchymal Stem Cells in Combination with Low Molecular Weight Hyaluronic Acid for Cartilage Repair |
| title_full | Synovium-Derived Mesenchymal Stem Cells in Combination with Low Molecular Weight Hyaluronic Acid for Cartilage Repair |
| title_fullStr | Synovium-Derived Mesenchymal Stem Cells in Combination with Low Molecular Weight Hyaluronic Acid for Cartilage Repair |
| title_full_unstemmed | Synovium-Derived Mesenchymal Stem Cells in Combination with Low Molecular Weight Hyaluronic Acid for Cartilage Repair |
| title_short | Synovium-Derived Mesenchymal Stem Cells in Combination with Low Molecular Weight Hyaluronic Acid for Cartilage Repair |
| title_sort | synovium derived mesenchymal stem cells in combination with low molecular weight hyaluronic acid for cartilage repair |
| topic | mesenchymal stem cells hyaluronic acid growth factors cartilage defect regeneration cell therapy |
| url | http://www.ijbm.org/articles/i48/ijbm_12(4)_oa4.pdf |
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