Serological cytokine profiles of cardiac rejection and lung infection after heart transplantation in rats
Abstract Background Allograft rejection and infection are the major sources of morbidity and mortality after heart transplant. Early differential diagnosis is clinically crucial but difficult. The aim of the study was to examine serum cytokine profiles associated with each entity and whether such pr...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | Journal of Cardiothoracic Surgery |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13019-019-0839-5 |
| _version_ | 1818160416683982848 |
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| author | Hao Chen Juhua Yang Shengchao Zhang Xuan Qin Wei Jin Lihua Sun Feng Li Yunfeng Cheng |
| author_facet | Hao Chen Juhua Yang Shengchao Zhang Xuan Qin Wei Jin Lihua Sun Feng Li Yunfeng Cheng |
| author_sort | Hao Chen |
| collection | DOAJ |
| description | Abstract Background Allograft rejection and infection are the major sources of morbidity and mortality after heart transplant. Early differential diagnosis is clinically crucial but difficult. The aim of the study was to examine serum cytokine profiles associated with each entity and whether such profiles could help to differentiate between them. Methods Heart allografts from Wistar rats were transplanted to Lewis rats as described by Yokoyama. Cardiac rejection and pulmonary bacterial infection were induced by Cyclosporine cessation and bacteria bronchus injection, and pathologically confirmed. Ninety serological cytokines profiles of the study objects were then simultaneously measured using a biotin label-based cytokine array. The fold change (FC) was used for relative cytokine concentration comparison analysis. Results Four cytokines in cardiac rejection group were significantly dysregulated as compared to health controls (β -Catenin, 0.51 FC; E-Selectin, 0.62 FC; IFN-gamma, 1.87 FC; and IL-13, 0.60 FC, respectively). In pulmonary infection animals, 11 cytokines were remarkably dysregulated in comparison with the control group (CINC-3, 0.57 FC; CNTF R alpha, 0.59 FC; E-Selectin, 0.58 FC; FSL1,0.62 FC; Hepassocin, 0.64 FC; IL-2, 0.26 FC; IL-13, 0.49 FC; NGFR, 0.57 FC; RAGE, 0.50 FC; TIMP-1, 0.49 FC; and IFN-gamma, 1.77 FC, respectively). Eleven cytokines were significantly up-regulated in cardiac rejection group comparing to the pulmonary infection animals (FSL1, 2.32FC; Fractalkine, 1.65FC; GFR alpha-1, 1.64FC; IL-2, 2.72FC; IL-5, 1.60FC; MMP-2, 1.71FC; NGFR, 2.25FC; TGF-beta1, 1.58FC; TGF-beta3, 1.58FC; Thrombospondin, 1.64FC, and TIMP-1, 1.52FC, respectively). Conclusions The current study illustrated the disease-specific serological cytokine profiles of allograft rejection and pulmonary bacterial infection after cardiac transplant. Such disease associated cytokine portraits might have the potential for early discrimination diagnosis. |
| first_indexed | 2024-12-11T16:01:32Z |
| format | Article |
| id | doaj.art-a12ae7c2bf01462b872acebe73a96d3b |
| institution | Directory Open Access Journal |
| issn | 1749-8090 |
| language | English |
| last_indexed | 2024-12-11T16:01:32Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
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| series | Journal of Cardiothoracic Surgery |
| spelling | doaj.art-a12ae7c2bf01462b872acebe73a96d3b2022-12-22T00:59:17ZengBMCJournal of Cardiothoracic Surgery1749-80902019-01-011411710.1186/s13019-019-0839-5Serological cytokine profiles of cardiac rejection and lung infection after heart transplantation in ratsHao Chen0Juhua Yang1Shengchao Zhang2Xuan Qin3Wei Jin4Lihua Sun5Feng Li6Yunfeng Cheng7Department of Thoracic Surgery, Zhongshan Hospital Qingpu Branch, Fudan UniversityDepartment of Thoracic Surgery, Zhongshan Hospital Qingpu Branch, Fudan UniversityDepartment of Thoracic Surgery, Zhongshan Hospital Qingpu Branch, Fudan UniversityDepartment of Thoracic Surgery, Zhongshan Hospital Qingpu Branch, Fudan UniversityDepartment of Thoracic Surgery, Zhongshan Hospital Qingpu Branch, Fudan UniversityDepartment of Hematology, Zhongshan Hospital Qingpu Branch, Fudan UniversityDepartment of Hematology, Zhongshan Hospital Qingpu Branch, Fudan UniversityDepartment of Hematology, Zhongshan Hospital Qingpu Branch, Fudan UniversityAbstract Background Allograft rejection and infection are the major sources of morbidity and mortality after heart transplant. Early differential diagnosis is clinically crucial but difficult. The aim of the study was to examine serum cytokine profiles associated with each entity and whether such profiles could help to differentiate between them. Methods Heart allografts from Wistar rats were transplanted to Lewis rats as described by Yokoyama. Cardiac rejection and pulmonary bacterial infection were induced by Cyclosporine cessation and bacteria bronchus injection, and pathologically confirmed. Ninety serological cytokines profiles of the study objects were then simultaneously measured using a biotin label-based cytokine array. The fold change (FC) was used for relative cytokine concentration comparison analysis. Results Four cytokines in cardiac rejection group were significantly dysregulated as compared to health controls (β -Catenin, 0.51 FC; E-Selectin, 0.62 FC; IFN-gamma, 1.87 FC; and IL-13, 0.60 FC, respectively). In pulmonary infection animals, 11 cytokines were remarkably dysregulated in comparison with the control group (CINC-3, 0.57 FC; CNTF R alpha, 0.59 FC; E-Selectin, 0.58 FC; FSL1,0.62 FC; Hepassocin, 0.64 FC; IL-2, 0.26 FC; IL-13, 0.49 FC; NGFR, 0.57 FC; RAGE, 0.50 FC; TIMP-1, 0.49 FC; and IFN-gamma, 1.77 FC, respectively). Eleven cytokines were significantly up-regulated in cardiac rejection group comparing to the pulmonary infection animals (FSL1, 2.32FC; Fractalkine, 1.65FC; GFR alpha-1, 1.64FC; IL-2, 2.72FC; IL-5, 1.60FC; MMP-2, 1.71FC; NGFR, 2.25FC; TGF-beta1, 1.58FC; TGF-beta3, 1.58FC; Thrombospondin, 1.64FC, and TIMP-1, 1.52FC, respectively). Conclusions The current study illustrated the disease-specific serological cytokine profiles of allograft rejection and pulmonary bacterial infection after cardiac transplant. Such disease associated cytokine portraits might have the potential for early discrimination diagnosis.http://link.springer.com/article/10.1186/s13019-019-0839-5Heart transplantAcute rejectionPulmonary infectionCytokine profileDifferential diagnosis |
| spellingShingle | Hao Chen Juhua Yang Shengchao Zhang Xuan Qin Wei Jin Lihua Sun Feng Li Yunfeng Cheng Serological cytokine profiles of cardiac rejection and lung infection after heart transplantation in rats Journal of Cardiothoracic Surgery Heart transplant Acute rejection Pulmonary infection Cytokine profile Differential diagnosis |
| title | Serological cytokine profiles of cardiac rejection and lung infection after heart transplantation in rats |
| title_full | Serological cytokine profiles of cardiac rejection and lung infection after heart transplantation in rats |
| title_fullStr | Serological cytokine profiles of cardiac rejection and lung infection after heart transplantation in rats |
| title_full_unstemmed | Serological cytokine profiles of cardiac rejection and lung infection after heart transplantation in rats |
| title_short | Serological cytokine profiles of cardiac rejection and lung infection after heart transplantation in rats |
| title_sort | serological cytokine profiles of cardiac rejection and lung infection after heart transplantation in rats |
| topic | Heart transplant Acute rejection Pulmonary infection Cytokine profile Differential diagnosis |
| url | http://link.springer.com/article/10.1186/s13019-019-0839-5 |
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