Unique Mitochondrial Single Nucleotide Polymorphisms Demonstrate Resolution Potential to Discriminate <i>Theileria parva</i> Vaccine and Buffalo-Derived Strains
Distinct pathogenic and epidemiological features underlie different <i>Theileria parva</i> strains resulting in different clinical manifestations of East Coast Fever and Corridor Disease in susceptible cattle. Unclear delineation of these strains limits the control of these diseases in e...
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MDPI AG
2020-12-01
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| Online toegang: | https://www.mdpi.com/2075-1729/10/12/334 |
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| author | Micky M. Mwamuye Isaiah Obara Khawla Elati David Odongo Mohammed A. Bakheit Frans Jongejan Ard M. Nijhof |
| author_facet | Micky M. Mwamuye Isaiah Obara Khawla Elati David Odongo Mohammed A. Bakheit Frans Jongejan Ard M. Nijhof |
| author_sort | Micky M. Mwamuye |
| collection | DOAJ |
| description | Distinct pathogenic and epidemiological features underlie different <i>Theileria parva</i> strains resulting in different clinical manifestations of East Coast Fever and Corridor Disease in susceptible cattle. Unclear delineation of these strains limits the control of these diseases in endemic areas. Hence, an accurate characterization of strains can improve the treatment and prevention approaches as well as investigate their origin. Here, we describe a set of single nucleotide polymorphisms (SNPs) based on 13 near-complete mitogenomes of <i>T. parva</i> strains originating from East and Southern Africa, including the live vaccine stock strains. We identified 11 SNPs that are non-preferentially distributed within the coding and non-coding regions, all of which are synonymous except for two within the <i>cytochrome b</i> gene of buffalo-derived strains. Our analysis ascertains haplotype-specific mutations that segregate the different vaccine and the buffalo-derived strains except <i>T. parva-</i>Muguga and Serengeti-transformed strains suggesting a shared lineage between the latter two vaccine strains. Phylogenetic analyses including the mitogenomes of other <i>Theileria</i> species: <i>T. annulata</i>, <i>T. taurotragi</i>, and <i>T. lestoquardi</i>, with the latter two sequenced in this study for the first time, were congruent with nuclear-encoded genes. Importantly, we describe seven <i>T. parva</i> haplotypes characterized by synonymous SNPs and parsimony-informative characters with the other three transforming species mitogenomes. We anticipate that tracking <i>T. parva</i> mitochondrial haplotypes from this study will provide insight into the parasite’s epidemiological dynamics and underpin current control efforts. |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T14:14:49Z |
| publishDate | 2020-12-01 |
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| series | Life |
| spelling | doaj.art-a12b76bd7c2844a8959d2d5f2863a9f42023-11-20T23:56:08ZengMDPI AGLife2075-17292020-12-01101233410.3390/life10120334Unique Mitochondrial Single Nucleotide Polymorphisms Demonstrate Resolution Potential to Discriminate <i>Theileria parva</i> Vaccine and Buffalo-Derived StrainsMicky M. Mwamuye0Isaiah Obara1Khawla Elati2David Odongo3Mohammed A. Bakheit4Frans Jongejan5Ard M. Nijhof6Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, GermanyInstitute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, GermanyInstitute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, GermanySchool of Biological Sciences, University of Nairobi, P.O. Box 30197-00100 Nairobi, KenyaDepartment of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 321-11115 Khartoum, SudanVectors and Vector-Borne Diseases Research Programme, Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, 0110 Onderstepoort, South AfricaInstitute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, GermanyDistinct pathogenic and epidemiological features underlie different <i>Theileria parva</i> strains resulting in different clinical manifestations of East Coast Fever and Corridor Disease in susceptible cattle. Unclear delineation of these strains limits the control of these diseases in endemic areas. Hence, an accurate characterization of strains can improve the treatment and prevention approaches as well as investigate their origin. Here, we describe a set of single nucleotide polymorphisms (SNPs) based on 13 near-complete mitogenomes of <i>T. parva</i> strains originating from East and Southern Africa, including the live vaccine stock strains. We identified 11 SNPs that are non-preferentially distributed within the coding and non-coding regions, all of which are synonymous except for two within the <i>cytochrome b</i> gene of buffalo-derived strains. Our analysis ascertains haplotype-specific mutations that segregate the different vaccine and the buffalo-derived strains except <i>T. parva-</i>Muguga and Serengeti-transformed strains suggesting a shared lineage between the latter two vaccine strains. Phylogenetic analyses including the mitogenomes of other <i>Theileria</i> species: <i>T. annulata</i>, <i>T. taurotragi</i>, and <i>T. lestoquardi</i>, with the latter two sequenced in this study for the first time, were congruent with nuclear-encoded genes. Importantly, we describe seven <i>T. parva</i> haplotypes characterized by synonymous SNPs and parsimony-informative characters with the other three transforming species mitogenomes. We anticipate that tracking <i>T. parva</i> mitochondrial haplotypes from this study will provide insight into the parasite’s epidemiological dynamics and underpin current control efforts.https://www.mdpi.com/2075-1729/10/12/334<i>Theileria parva</i>mitogenomeshaplotypesSNPslive vaccine |
| spellingShingle | Micky M. Mwamuye Isaiah Obara Khawla Elati David Odongo Mohammed A. Bakheit Frans Jongejan Ard M. Nijhof Unique Mitochondrial Single Nucleotide Polymorphisms Demonstrate Resolution Potential to Discriminate <i>Theileria parva</i> Vaccine and Buffalo-Derived Strains Life <i>Theileria parva</i> mitogenomes haplotypes SNPs live vaccine |
| title | Unique Mitochondrial Single Nucleotide Polymorphisms Demonstrate Resolution Potential to Discriminate <i>Theileria parva</i> Vaccine and Buffalo-Derived Strains |
| title_full | Unique Mitochondrial Single Nucleotide Polymorphisms Demonstrate Resolution Potential to Discriminate <i>Theileria parva</i> Vaccine and Buffalo-Derived Strains |
| title_fullStr | Unique Mitochondrial Single Nucleotide Polymorphisms Demonstrate Resolution Potential to Discriminate <i>Theileria parva</i> Vaccine and Buffalo-Derived Strains |
| title_full_unstemmed | Unique Mitochondrial Single Nucleotide Polymorphisms Demonstrate Resolution Potential to Discriminate <i>Theileria parva</i> Vaccine and Buffalo-Derived Strains |
| title_short | Unique Mitochondrial Single Nucleotide Polymorphisms Demonstrate Resolution Potential to Discriminate <i>Theileria parva</i> Vaccine and Buffalo-Derived Strains |
| title_sort | unique mitochondrial single nucleotide polymorphisms demonstrate resolution potential to discriminate i theileria parva i vaccine and buffalo derived strains |
| topic | <i>Theileria parva</i> mitogenomes haplotypes SNPs live vaccine |
| url | https://www.mdpi.com/2075-1729/10/12/334 |
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