Preparation, Mechanical Properties, and Biocompatibility of Graphene Oxide-Reinforced Chitin Monofilament Absorbable Surgical Sutures
Chitin (CT) is a good material to prepare surgical sutures due to its conspicuous biological characteristics. However, the poor mechanical strength of pure CT sutures limits its application. In order to improve its strength, a composite monofilament absorbable suture was prepared in this study using...
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MDPI AG
2019-04-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/4/210 |
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| author | Wei Zhang Bin Yin Yu Xin Lei Li Guanlin Ye Junxian Wang Jianfei Shen Xiao Cui Qihui Yang |
| author_facet | Wei Zhang Bin Yin Yu Xin Lei Li Guanlin Ye Junxian Wang Jianfei Shen Xiao Cui Qihui Yang |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | Chitin (CT) is a good material to prepare surgical sutures due to its conspicuous biological characteristics. However, the poor mechanical strength of pure CT sutures limits its application. In order to improve its strength, a composite monofilament absorbable suture was prepared in this study using graphene oxide and chitin (GO-CT) using a green method. FT-IR spectra showed that GO-CT contained the characteristic functional groups of GO and CT, indicating that a GO-CT suture was successfully obtained. With the addition of a small amount of GO (1.6wt% solution) in chitin, the breaking tensile strength, knot strength, and knot-pull strength of the GO-CT suture were significantly improved compared to the CT suture. The biocompatibility of the GO-CT suture in vitro was checked by tetrazolium-based colorimetric assays and no cytotoxicity to L929 cells was found. In vivo, the subcutaneous implantation of GO-CT sutures in the dorsal skin of rats found no abnormalities by hematoxylin-eosin staining. Furthermore, there were no significant changes in the gene expression of the inflammatory mediators, interleukin 1β (<i>IL-1β</i>), tumor necrosis factor-<i>α</i>, <i>IL-6</i>, <i>IL-17A</i>, interferon-<i>γ</i>, or <i>IL-10</i>; however, the expression of transforming growth factor β was significantly increased in the first week. In summary, GO-CT sutures may have potential as a suture material in the clinic. |
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| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T22:51:59Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj.art-a12b7d39bc08451a94f31bb9deb9eba92022-12-22T03:58:33ZengMDPI AGMarine Drugs1660-33972019-04-0117421010.3390/md17040210md17040210Preparation, Mechanical Properties, and Biocompatibility of Graphene Oxide-Reinforced Chitin Monofilament Absorbable Surgical SuturesWei Zhang0Bin Yin1Yu Xin2Lei Li3Guanlin Ye4Junxian Wang5Jianfei Shen6Xiao Cui7Qihui Yang8College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524025, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524025, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524025, ChinaLife Science and Technology School, Lingnan Normal University, Zhanjiang 524048, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524025, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524025, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524025, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524025, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524025, ChinaChitin (CT) is a good material to prepare surgical sutures due to its conspicuous biological characteristics. However, the poor mechanical strength of pure CT sutures limits its application. In order to improve its strength, a composite monofilament absorbable suture was prepared in this study using graphene oxide and chitin (GO-CT) using a green method. FT-IR spectra showed that GO-CT contained the characteristic functional groups of GO and CT, indicating that a GO-CT suture was successfully obtained. With the addition of a small amount of GO (1.6wt% solution) in chitin, the breaking tensile strength, knot strength, and knot-pull strength of the GO-CT suture were significantly improved compared to the CT suture. The biocompatibility of the GO-CT suture in vitro was checked by tetrazolium-based colorimetric assays and no cytotoxicity to L929 cells was found. In vivo, the subcutaneous implantation of GO-CT sutures in the dorsal skin of rats found no abnormalities by hematoxylin-eosin staining. Furthermore, there were no significant changes in the gene expression of the inflammatory mediators, interleukin 1β (<i>IL-1β</i>), tumor necrosis factor-<i>α</i>, <i>IL-6</i>, <i>IL-17A</i>, interferon-<i>γ</i>, or <i>IL-10</i>; however, the expression of transforming growth factor β was significantly increased in the first week. In summary, GO-CT sutures may have potential as a suture material in the clinic.https://www.mdpi.com/1660-3397/17/4/210graphene oxidechitinsuturebiocompatibilitymechanical strength |
| spellingShingle | Wei Zhang Bin Yin Yu Xin Lei Li Guanlin Ye Junxian Wang Jianfei Shen Xiao Cui Qihui Yang Preparation, Mechanical Properties, and Biocompatibility of Graphene Oxide-Reinforced Chitin Monofilament Absorbable Surgical Sutures Marine Drugs graphene oxide chitin suture biocompatibility mechanical strength |
| title | Preparation, Mechanical Properties, and Biocompatibility of Graphene Oxide-Reinforced Chitin Monofilament Absorbable Surgical Sutures |
| title_full | Preparation, Mechanical Properties, and Biocompatibility of Graphene Oxide-Reinforced Chitin Monofilament Absorbable Surgical Sutures |
| title_fullStr | Preparation, Mechanical Properties, and Biocompatibility of Graphene Oxide-Reinforced Chitin Monofilament Absorbable Surgical Sutures |
| title_full_unstemmed | Preparation, Mechanical Properties, and Biocompatibility of Graphene Oxide-Reinforced Chitin Monofilament Absorbable Surgical Sutures |
| title_short | Preparation, Mechanical Properties, and Biocompatibility of Graphene Oxide-Reinforced Chitin Monofilament Absorbable Surgical Sutures |
| title_sort | preparation mechanical properties and biocompatibility of graphene oxide reinforced chitin monofilament absorbable surgical sutures |
| topic | graphene oxide chitin suture biocompatibility mechanical strength |
| url | https://www.mdpi.com/1660-3397/17/4/210 |
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