Identification and expression analysis of aquaporins in the potato psyllid, Bactericera cockerelli.
Aquaporin (AQPs) proteins transport water and uncharged low molecular-weight solutes across biological membranes. Six to 8 AQP genes have been identified in many insect species, but presently only three aquaporins have been characterized in phloem feeding insects. The objective of this study was to...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4213062?pdf=render |
| _version_ | 1818863943531102208 |
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| author | Freddy Ibanez Joseph Hancock Cecilia Tamborindeguy |
| author_facet | Freddy Ibanez Joseph Hancock Cecilia Tamborindeguy |
| author_sort | Freddy Ibanez |
| collection | DOAJ |
| description | Aquaporin (AQPs) proteins transport water and uncharged low molecular-weight solutes across biological membranes. Six to 8 AQP genes have been identified in many insect species, but presently only three aquaporins have been characterized in phloem feeding insects. The objective of this study was to identify candidate AQPs in the potato psyllid, Bactericera cockerelli. Herein, we identified four candidate aquaporin cDNAs in B. cockerelli transcriptome. Phylogenetic analysis showed that candidate BcAQP2-like had high similarity to PRIP aquaporins; while candidates BcAQP4-like, BcAQP5-like and BcAQP9-like clustered within clade B. In particular, candidates BcAQP4-like and BcAQP5-like clustered with functionally validated insect aquaglyceroporin proteins. Expression analyses using RT-qPCR showed that all candidates were expressed in all life stages and tissues. Candidates BcAQP4-like and BcAQP5-like were highly expressed in bacteriocytes, while BcAQP9-like appeared to be expressed at high levels in whole body but not in the assayed tissues. This study is the first global attempt to identify putative aquaporins in a phloem feeding insect. |
| first_indexed | 2024-12-19T10:23:47Z |
| format | Article |
| id | doaj.art-a12d3a980e9f48a9b5832c3d3e4ad42d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T10:23:47Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a12d3a980e9f48a9b5832c3d3e4ad42d2022-12-21T20:25:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11174510.1371/journal.pone.0111745Identification and expression analysis of aquaporins in the potato psyllid, Bactericera cockerelli.Freddy IbanezJoseph HancockCecilia TamborindeguyAquaporin (AQPs) proteins transport water and uncharged low molecular-weight solutes across biological membranes. Six to 8 AQP genes have been identified in many insect species, but presently only three aquaporins have been characterized in phloem feeding insects. The objective of this study was to identify candidate AQPs in the potato psyllid, Bactericera cockerelli. Herein, we identified four candidate aquaporin cDNAs in B. cockerelli transcriptome. Phylogenetic analysis showed that candidate BcAQP2-like had high similarity to PRIP aquaporins; while candidates BcAQP4-like, BcAQP5-like and BcAQP9-like clustered within clade B. In particular, candidates BcAQP4-like and BcAQP5-like clustered with functionally validated insect aquaglyceroporin proteins. Expression analyses using RT-qPCR showed that all candidates were expressed in all life stages and tissues. Candidates BcAQP4-like and BcAQP5-like were highly expressed in bacteriocytes, while BcAQP9-like appeared to be expressed at high levels in whole body but not in the assayed tissues. This study is the first global attempt to identify putative aquaporins in a phloem feeding insect.http://europepmc.org/articles/PMC4213062?pdf=render |
| spellingShingle | Freddy Ibanez Joseph Hancock Cecilia Tamborindeguy Identification and expression analysis of aquaporins in the potato psyllid, Bactericera cockerelli. PLoS ONE |
| title | Identification and expression analysis of aquaporins in the potato psyllid, Bactericera cockerelli. |
| title_full | Identification and expression analysis of aquaporins in the potato psyllid, Bactericera cockerelli. |
| title_fullStr | Identification and expression analysis of aquaporins in the potato psyllid, Bactericera cockerelli. |
| title_full_unstemmed | Identification and expression analysis of aquaporins in the potato psyllid, Bactericera cockerelli. |
| title_short | Identification and expression analysis of aquaporins in the potato psyllid, Bactericera cockerelli. |
| title_sort | identification and expression analysis of aquaporins in the potato psyllid bactericera cockerelli |
| url | http://europepmc.org/articles/PMC4213062?pdf=render |
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