Effect Combination of Catharanthus roseusand Phyllanthus urinariaExtract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression
CD4+CD25+FOXP3+is a regulatory T cell that plays a role in the body's tolerant sys-tem to control activated effector cells. T regulatory plays an important role as a trigger for tumor progressiveness, where an increase in the amount of T regulatory FOXP3 in the tumor state correlates with a poo...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2022-05-01
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| Series: | Journal of Tropical Life Science |
| Subjects: | |
| Online Access: | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2298/588 |
| _version_ | 1797410239691096064 |
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| author | Aya Shofiyah M. Sasmito Djati Muhaimin Rifa’I |
| author_facet | Aya Shofiyah M. Sasmito Djati Muhaimin Rifa’I |
| author_sort | Aya Shofiyah |
| collection | DOAJ |
| description | CD4+CD25+FOXP3+is a regulatory T cell that plays a role in the body's tolerant sys-tem to control activated effector cells. T regulatory plays an important role as a trigger for tumor progressiveness, where an increase in the amount of T regulatory FOXP3 in the tumor state correlates with a poor prognosis. Breast cancer is a type of cancer that tends to increase every year. The purpose of this study was to study the influence of a combination of Phyllanthus urinariaand Catharanthus roseusplant extracts on CD4+CD25+FOXP3+modulation. Mice was injected subcutaneously with the carcin-ogen compound DMBA for six weeks at 0.015 mg/kg weight. The study design was conducted using a complete random design (RAL) which was divided into six groups namely healthy (normal) control, cancer control (DMBA injection), Cisplatin and a combination of extracts with three different doses (dose 1 (Phyllanthus urinaria500 weight and Catharanthus roseus75mg/kg weight) and dose 3 (Phyllanthus urinary2000mg/kg weight and Catharanthus roseus375mg/kg weight). The relative number of T regulatory cells is measured using flow cytometry analysis. The measurements were made using the Cell Quest ProTMprogram. Statistics analyze using one way ANOVA (p<0.05). Tukey HSD analysis with SPSS 26 software used to analyze real differences between treatments. A relative increase in CD4+CD25+FOXP3+occurs in cancer control. While the decrease occurred in every treatment except in the dose group 1. The optimal dose to reduce CD4+CD25+FOXP3+amounts significantly oc-curred in the dose group 3. This indicates the influence of a combination of Phyl-lanthus urinariaand Catharanthus roseuson CD4+CD25+FOXP3+differentiation. |
| first_indexed | 2024-03-09T04:26:02Z |
| format | Article |
| id | doaj.art-a12f0af86abd40d0816d83df7b66deed |
| institution | Directory Open Access Journal |
| issn | 2087-5517 |
| language | English |
| last_indexed | 2024-03-09T04:26:02Z |
| publishDate | 2022-05-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-a12f0af86abd40d0816d83df7b66deed2023-12-03T13:40:10ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172022-05-01122253260http://dx.doi.org/10.11594/jtls.12.02.11Effect Combination of Catharanthus roseusand Phyllanthus urinariaExtract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells ExpressionAya Shofiyah0M. Sasmito Djati1 Muhaimin Rifa’I2Department of Biology, Faculty of Mathematic and Natural Science, University of Brawijaya, Malang, 65154, IndonesiaDepartment of Biology, Faculty of Mathematic and Natural Science, University of Brawijaya, Malang, 65154, IndonesiaDepartment of Biology, Faculty of Mathematic and Natural Science, University of Brawijaya, Malang, 65154, IndonesiaCD4+CD25+FOXP3+is a regulatory T cell that plays a role in the body's tolerant sys-tem to control activated effector cells. T regulatory plays an important role as a trigger for tumor progressiveness, where an increase in the amount of T regulatory FOXP3 in the tumor state correlates with a poor prognosis. Breast cancer is a type of cancer that tends to increase every year. The purpose of this study was to study the influence of a combination of Phyllanthus urinariaand Catharanthus roseusplant extracts on CD4+CD25+FOXP3+modulation. Mice was injected subcutaneously with the carcin-ogen compound DMBA for six weeks at 0.015 mg/kg weight. The study design was conducted using a complete random design (RAL) which was divided into six groups namely healthy (normal) control, cancer control (DMBA injection), Cisplatin and a combination of extracts with three different doses (dose 1 (Phyllanthus urinaria500 weight and Catharanthus roseus75mg/kg weight) and dose 3 (Phyllanthus urinary2000mg/kg weight and Catharanthus roseus375mg/kg weight). The relative number of T regulatory cells is measured using flow cytometry analysis. The measurements were made using the Cell Quest ProTMprogram. Statistics analyze using one way ANOVA (p<0.05). Tukey HSD analysis with SPSS 26 software used to analyze real differences between treatments. A relative increase in CD4+CD25+FOXP3+occurs in cancer control. While the decrease occurred in every treatment except in the dose group 1. The optimal dose to reduce CD4+CD25+FOXP3+amounts significantly oc-curred in the dose group 3. This indicates the influence of a combination of Phyl-lanthus urinariaand Catharanthus roseuson CD4+CD25+FOXP3+differentiation. https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2298/588breast cancercatharanthus roseuscd4+cd25+foxp3+phyllanthus urinaria |
| spellingShingle | Aya Shofiyah M. Sasmito Djati Muhaimin Rifa’I Effect Combination of Catharanthus roseusand Phyllanthus urinariaExtract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression Journal of Tropical Life Science breast cancer catharanthus roseus cd4+cd25+foxp3+ phyllanthus urinaria |
| title | Effect Combination of Catharanthus roseusand Phyllanthus urinariaExtract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression |
| title_full | Effect Combination of Catharanthus roseusand Phyllanthus urinariaExtract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression |
| title_fullStr | Effect Combination of Catharanthus roseusand Phyllanthus urinariaExtract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression |
| title_full_unstemmed | Effect Combination of Catharanthus roseusand Phyllanthus urinariaExtract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression |
| title_short | Effect Combination of Catharanthus roseusand Phyllanthus urinariaExtract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression |
| title_sort | effect combination of catharanthus roseusand phyllanthus urinariaextract on mice balb c breast cancer models based on t regulator cells expression |
| topic | breast cancer catharanthus roseus cd4+cd25+foxp3+ phyllanthus urinaria |
| url | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2298/588 |
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