Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration
Three-dimensionally printed porous titanium alloys prepared using the electron beam melting (EBM) technology have customized structures and a low elastic modulus. They can promote the repair of bone defects. Growth factors can enhance the biological activity of plants in vivo, and bone morphogenetic...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127521004354 |
| _version_ | 1819139938900246528 |
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| author | Zhengqing Zhu Xin Li Youbin Li Liwei Zhu Chenyi Zhu Zhenjia Che Lanfeng Huang |
| author_facet | Zhengqing Zhu Xin Li Youbin Li Liwei Zhu Chenyi Zhu Zhenjia Che Lanfeng Huang |
| author_sort | Zhengqing Zhu |
| collection | DOAJ |
| description | Three-dimensionally printed porous titanium alloys prepared using the electron beam melting (EBM) technology have customized structures and a low elastic modulus. They can promote the repair of bone defects. Growth factors can enhance the biological activity of plants in vivo, and bone morphogenetic protein 9 (BMP9) is the key factor for osteogenesis. The purpose of this study was to evaluate a porous titanium implant prepared using the EBM technology with temperature-sensitive collagen mixed with recombinant human (rh) BMP9 as a composite scaffold to be continuously provided to enhance osteogenesis in rabbit femoral defects. The in vitro experiments verified cell proliferation, proliferation and application of the composite stent, the degree of release of temperature-sensitive collagen to rhBMP9, and the osteogenesis-inducing effect of the composite stent on bone marrow mesenchymal stem cells. In vivo, the rabbit femoral defect model was used to evaluate the effects of the composite on osteointegration and bone ingrowth. The results showed that the composite scaffold had good biocompatibility and provided bioactive growth factors for bone repair. Further, the release of rhBMP9 significantly enhanced osteogenesis in and around the porous scaffolds. This method helps to study bioactive coating on the titanium alloy surface of patients with bone defects. |
| first_indexed | 2024-12-22T11:30:37Z |
| format | Article |
| id | doaj.art-a13077f7aaca41b3b47cf3fd74cb39d0 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-12-22T11:30:37Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-a13077f7aaca41b3b47cf3fd74cb39d02022-12-21T18:27:37ZengElsevierMaterials & Design0264-12752021-10-01208109882Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegrationZhengqing Zhu0Xin Li1Youbin Li2Liwei Zhu3Chenyi Zhu4Zhenjia Che5Lanfeng Huang6Department of Orthopaedics, The Second Hospital of Jilin University, Changchun 130041, Jilin, ChinaDepartment of Orthopaedics, The Second Hospital of Jilin University, Changchun 130041, Jilin, ChinaDepartment of Orthopaedics, The Second Hospital of Jilin University, Changchun 130041, Jilin, ChinaDepartment of Orthopaedics, The Second Hospital of Jilin University, Changchun 130041, Jilin, ChinaDepartment of Orthopaedics, The Second Hospital of Jilin University, Changchun 130041, Jilin, ChinaDepartment of Orthopaedics, The Second Hospital of Jilin University, Changchun 130041, Jilin, ChinaCorresponding author.; Department of Orthopaedics, The Second Hospital of Jilin University, Changchun 130041, Jilin, ChinaThree-dimensionally printed porous titanium alloys prepared using the electron beam melting (EBM) technology have customized structures and a low elastic modulus. They can promote the repair of bone defects. Growth factors can enhance the biological activity of plants in vivo, and bone morphogenetic protein 9 (BMP9) is the key factor for osteogenesis. The purpose of this study was to evaluate a porous titanium implant prepared using the EBM technology with temperature-sensitive collagen mixed with recombinant human (rh) BMP9 as a composite scaffold to be continuously provided to enhance osteogenesis in rabbit femoral defects. The in vitro experiments verified cell proliferation, proliferation and application of the composite stent, the degree of release of temperature-sensitive collagen to rhBMP9, and the osteogenesis-inducing effect of the composite stent on bone marrow mesenchymal stem cells. In vivo, the rabbit femoral defect model was used to evaluate the effects of the composite on osteointegration and bone ingrowth. The results showed that the composite scaffold had good biocompatibility and provided bioactive growth factors for bone repair. Further, the release of rhBMP9 significantly enhanced osteogenesis in and around the porous scaffolds. This method helps to study bioactive coating on the titanium alloy surface of patients with bone defects.http://www.sciencedirect.com/science/article/pii/S0264127521004354Bone morphogenetic proteinBioactive interface3D-printed porous titanium scaffoldOsseointegrationBone ingrowth |
| spellingShingle | Zhengqing Zhu Xin Li Youbin Li Liwei Zhu Chenyi Zhu Zhenjia Che Lanfeng Huang Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration Materials & Design Bone morphogenetic protein Bioactive interface 3D-printed porous titanium scaffold Osseointegration Bone ingrowth |
| title | Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration |
| title_full | Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration |
| title_fullStr | Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration |
| title_full_unstemmed | Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration |
| title_short | Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration |
| title_sort | three dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration |
| topic | Bone morphogenetic protein Bioactive interface 3D-printed porous titanium scaffold Osseointegration Bone ingrowth |
| url | http://www.sciencedirect.com/science/article/pii/S0264127521004354 |
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