The adipokines progranulin and omentin – novel regulators of basic ovarian cell functions
Abstract The present study aimed to examine the effects of progranulin and omentin on basic ovarian cell functions. For this purpose, we investigated the effects of the addition of progranulin and omentin (0, 0.1, 1, or 10 ng/ml) on the viability, proliferation, apoptosis and steroidogenesis of cult...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12958-024-01215-9 |
| _version_ | 1797219475588644864 |
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| author | Alexander V Sirotkin Zuzana Fabová Barbora Loncová Maria Bauerová Abdel Halim Harrath |
| author_facet | Alexander V Sirotkin Zuzana Fabová Barbora Loncová Maria Bauerová Abdel Halim Harrath |
| author_sort | Alexander V Sirotkin |
| collection | DOAJ |
| description | Abstract The present study aimed to examine the effects of progranulin and omentin on basic ovarian cell functions. For this purpose, we investigated the effects of the addition of progranulin and omentin (0, 0.1, 1, or 10 ng/ml) on the viability, proliferation, apoptosis and steroidogenesis of cultured rabbit ovarian granulosa cells. To determine the importance of the interrelationships between granulosa cells and theca cells, we compared the influence of progranulin and omentin on progesterone and estradiol release in cultured granulosa cells and ovarian fragments containing both granulosa cells and theca cells. Cell viability, proliferation, cytoplasmic apoptosis and release of progesterone and estradiol were measured by Cell Counting Kit-8 (CCK-8), BrdU incorporation, cell death detection, and ELISA. Both progranulin and omentin increased granulosa cell viability and proliferation and decreased apoptosis. Progranulin increased progesterone release by granulosa cells but reduced progesterone output by ovarian fragments. Progranulin decreased estradiol release by granulosa cells but increased it in ovarian fragments. Omentin reduced progesterone release in both models. Omentin reduced estradiol release by granulosa cells but promoted this release in ovarian fragments. The present observations are the first to demonstrate that progranulin and omentin can be direct regulators of basic ovarian cell functions. Furthermore, the differences in the effects of these adipokines on steroidogenesis via granulosa and ovarian fragments indicate that these peptides could target both granulosa and theca cells. |
| first_indexed | 2024-04-24T12:34:14Z |
| format | Article |
| id | doaj.art-a1313d015451430ca60beae4abbf4443 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-04-24T12:34:14Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-a1313d015451430ca60beae4abbf44432024-04-07T11:34:30ZengBMCReproductive Biology and Endocrinology1477-78272024-04-012211710.1186/s12958-024-01215-9The adipokines progranulin and omentin – novel regulators of basic ovarian cell functionsAlexander V Sirotkin0Zuzana Fabová1Barbora Loncová2Maria Bauerová3Abdel Halim Harrath4Faculty of Natural Sciences, Constantine the Philosopher UniversityFaculty of Natural Sciences, Constantine the Philosopher UniversityFaculty of Natural Sciences, Constantine the Philosopher UniversityFaculty of Natural Sciences, Constantine the Philosopher UniversityDepartment of Zoology, College of Science, King Saud UniversityAbstract The present study aimed to examine the effects of progranulin and omentin on basic ovarian cell functions. For this purpose, we investigated the effects of the addition of progranulin and omentin (0, 0.1, 1, or 10 ng/ml) on the viability, proliferation, apoptosis and steroidogenesis of cultured rabbit ovarian granulosa cells. To determine the importance of the interrelationships between granulosa cells and theca cells, we compared the influence of progranulin and omentin on progesterone and estradiol release in cultured granulosa cells and ovarian fragments containing both granulosa cells and theca cells. Cell viability, proliferation, cytoplasmic apoptosis and release of progesterone and estradiol were measured by Cell Counting Kit-8 (CCK-8), BrdU incorporation, cell death detection, and ELISA. Both progranulin and omentin increased granulosa cell viability and proliferation and decreased apoptosis. Progranulin increased progesterone release by granulosa cells but reduced progesterone output by ovarian fragments. Progranulin decreased estradiol release by granulosa cells but increased it in ovarian fragments. Omentin reduced progesterone release in both models. Omentin reduced estradiol release by granulosa cells but promoted this release in ovarian fragments. The present observations are the first to demonstrate that progranulin and omentin can be direct regulators of basic ovarian cell functions. Furthermore, the differences in the effects of these adipokines on steroidogenesis via granulosa and ovarian fragments indicate that these peptides could target both granulosa and theca cells.https://doi.org/10.1186/s12958-024-01215-9ProgranulinOmentinOvaryHormonesApoptosisProliferation |
| spellingShingle | Alexander V Sirotkin Zuzana Fabová Barbora Loncová Maria Bauerová Abdel Halim Harrath The adipokines progranulin and omentin – novel regulators of basic ovarian cell functions Reproductive Biology and Endocrinology Progranulin Omentin Ovary Hormones Apoptosis Proliferation |
| title | The adipokines progranulin and omentin – novel regulators of basic ovarian cell functions |
| title_full | The adipokines progranulin and omentin – novel regulators of basic ovarian cell functions |
| title_fullStr | The adipokines progranulin and omentin – novel regulators of basic ovarian cell functions |
| title_full_unstemmed | The adipokines progranulin and omentin – novel regulators of basic ovarian cell functions |
| title_short | The adipokines progranulin and omentin – novel regulators of basic ovarian cell functions |
| title_sort | adipokines progranulin and omentin novel regulators of basic ovarian cell functions |
| topic | Progranulin Omentin Ovary Hormones Apoptosis Proliferation |
| url | https://doi.org/10.1186/s12958-024-01215-9 |
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