Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle
Mutualism is defined as a beneficial relationship for the associated partners and usually assumes that the symbiont number is controlled. Some trypanosomatid protozoa co-evolve with a bacterial symbiont that divides in coordination with the host in a way that results in its equal distribution betwee...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00520/full |
| _version_ | 1818982195875807232 |
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| author | Carolina Moura Costa Catta Preta Felipe eBrum Camila eDa Silva Aline eZuma Maria Carolina eElias Wanderley eDe Souza Sergio eSchenkman Maria Cristina Machado Motta |
| author_facet | Carolina Moura Costa Catta Preta Felipe eBrum Camila eDa Silva Aline eZuma Maria Carolina eElias Wanderley eDe Souza Sergio eSchenkman Maria Cristina Machado Motta |
| author_sort | Carolina Moura Costa Catta Preta |
| collection | DOAJ |
| description | Mutualism is defined as a beneficial relationship for the associated partners and usually assumes that the symbiont number is controlled. Some trypanosomatid protozoa co-evolve with a bacterial symbiont that divides in coordination with the host in a way that results in its equal distribution between daughter cells. The mechanism that controls this synchrony is largely unknown, and its comprehension might provide clues to understand how eukaryotic cells evolved when acquiring symbionts that later became organelles. Here, we approached this question by studying the effects of inhibitors that affect the host exclusively in two symbiont-bearing trypanosomatids, Strigomonas culicis and Angomonas deanei. We found that inhibiting host protein synthesis using cycloheximide or host DNA replication using aphidicolin did not affect the duplication of bacterial DNA. Although the bacteria had autonomy to duplicate their DNA when host protein synthesis was blocked by cycloheximide, they could not complete cytokinesis. Aphidicolin promoted the inhibition of the trypanosomatid cell cycle in the G1/S phase, leading to symbiont filamentation in S. culicis but not in A. deanei. Treatment with camptothecin blocked the host protozoa cell cycle in the G2 phase and induced the formation of filamentous symbionts in both species. Oryzalin, which affects host microtubule polymerization, blocked trypanosomatid mitosis and abrogated symbiont division. Our results indicate that host factors produced during the cell division cycle are essential for symbiont segregation and may control the bacterial cell number. |
| first_indexed | 2024-12-20T17:43:22Z |
| format | Article |
| id | doaj.art-a13150c5d2c14521a010cb37800c9f70 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T17:43:22Z |
| publishDate | 2015-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a13150c5d2c14521a010cb37800c9f702022-12-21T19:31:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-06-01610.3389/fmicb.2015.00520130220Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycleCarolina Moura Costa Catta Preta0Felipe eBrum1Camila eDa Silva2Aline eZuma3Maria Carolina eElias4Wanderley eDe Souza5Sergio eSchenkman6Maria Cristina Machado Motta7Universidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroButantanUniversidade Federal do Rio de JaneiroUniversidade Federal de São PauloUniversidade Federal do Rio de JaneiroMutualism is defined as a beneficial relationship for the associated partners and usually assumes that the symbiont number is controlled. Some trypanosomatid protozoa co-evolve with a bacterial symbiont that divides in coordination with the host in a way that results in its equal distribution between daughter cells. The mechanism that controls this synchrony is largely unknown, and its comprehension might provide clues to understand how eukaryotic cells evolved when acquiring symbionts that later became organelles. Here, we approached this question by studying the effects of inhibitors that affect the host exclusively in two symbiont-bearing trypanosomatids, Strigomonas culicis and Angomonas deanei. We found that inhibiting host protein synthesis using cycloheximide or host DNA replication using aphidicolin did not affect the duplication of bacterial DNA. Although the bacteria had autonomy to duplicate their DNA when host protein synthesis was blocked by cycloheximide, they could not complete cytokinesis. Aphidicolin promoted the inhibition of the trypanosomatid cell cycle in the G1/S phase, leading to symbiont filamentation in S. culicis but not in A. deanei. Treatment with camptothecin blocked the host protozoa cell cycle in the G2 phase and induced the formation of filamentous symbionts in both species. Oryzalin, which affects host microtubule polymerization, blocked trypanosomatid mitosis and abrogated symbiont division. Our results indicate that host factors produced during the cell division cycle are essential for symbiont segregation and may control the bacterial cell number.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00520/fullCell CycleSymbiosisTrypanosomatid protozoaCell evolutiondivision control |
| spellingShingle | Carolina Moura Costa Catta Preta Felipe eBrum Camila eDa Silva Aline eZuma Maria Carolina eElias Wanderley eDe Souza Sergio eSchenkman Maria Cristina Machado Motta Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle Frontiers in Microbiology Cell Cycle Symbiosis Trypanosomatid protozoa Cell evolution division control |
| title | Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle |
| title_full | Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle |
| title_fullStr | Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle |
| title_full_unstemmed | Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle |
| title_short | Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle |
| title_sort | endosymbiosis in trypanosomatid protozoa the bacterium division is controlled during the host cell cycle |
| topic | Cell Cycle Symbiosis Trypanosomatid protozoa Cell evolution division control |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00520/full |
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