Evaluation of the Diagnostic Potential of Recombinant <i>Coxiella burnetii</i> Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle
<i>Coxiella burnetii</i> is the causative agent of Q fever, a zoonosis infecting domestic ruminants and humans. Currently used routine diagnostic tools offer limited sensitivity and specificity and symptomless infected animals may be missed. Therefore, diagnostic tools of higher sensitiv...
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MDPI AG
2020-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/8/1235 |
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| author | Mareike Stellfeld Claudia Gerlach Ina-Gabriele Richter Peter Miethe Dominika Fahlbusch Birgitta Polley Reinhard Sting Martin Pfeffer Heinrich Neubauer Katja Mertens-Scholz |
| author_facet | Mareike Stellfeld Claudia Gerlach Ina-Gabriele Richter Peter Miethe Dominika Fahlbusch Birgitta Polley Reinhard Sting Martin Pfeffer Heinrich Neubauer Katja Mertens-Scholz |
| author_sort | Mareike Stellfeld |
| collection | DOAJ |
| description | <i>Coxiella burnetii</i> is the causative agent of Q fever, a zoonosis infecting domestic ruminants and humans. Currently used routine diagnostic tools offer limited sensitivity and specificity and symptomless infected animals may be missed. Therefore, diagnostic tools of higher sensitivity and specificity must be developed. For this purpose, the <i>C. burnetii</i> outer membrane protein Com1 was cloned and expressed in <i>Escherichia coli</i>. The His-tagged recombinant protein was purified and used in an indirect enzyme-linked immunosorbent assay (ELISA). Assay performance was tested with more than 400 positive and negative sera from sheep, goats and cattle from 36 locations. Calculation of sensitivity and specificity was undertaken using receiver operating characteristic (ROC) curves. The sensitivities and specificities for sheep were 85% and 68% (optical density at 450nm, OD<sub>450</sub> cut-off value 0.32), for goats 94% and 77% (OD<sub>450</sub> cut-off value 0.23) and for cattle 71% and 70% (OD<sub>450</sub> cut-off value 0.18), respectively. These results correspond to excellent, outstanding and acceptable discrimination of positive and negative sera. In summary, recombinant Com1 can provide a basis for more sensitive and specific diagnostic tools in veterinary medicine. |
| first_indexed | 2024-03-10T17:28:37Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T17:28:37Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a131973b58b04b0eb177ad2f06d452812023-11-20T10:05:56ZengMDPI AGMicroorganisms2076-26072020-08-0188123510.3390/microorganisms8081235Evaluation of the Diagnostic Potential of Recombinant <i>Coxiella burnetii</i> Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and CattleMareike Stellfeld0Claudia Gerlach1Ina-Gabriele Richter2Peter Miethe3Dominika Fahlbusch4Birgitta Polley5Reinhard Sting6Martin Pfeffer7Heinrich Neubauer8Katja Mertens-Scholz9Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, 07743 Jena, GermanyFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, 07743 Jena, GermanyResearch Centre of Medical Technology and Biotechnology (Forschungszentrum für Medizintechnik und Biotechnologie GmbH), 99947 Bad Langensalza, GermanyResearch Centre of Medical Technology and Biotechnology (Forschungszentrum für Medizintechnik und Biotechnologie GmbH), 99947 Bad Langensalza, GermanyResearch Centre of Medical Technology and Biotechnology (Forschungszentrum für Medizintechnik und Biotechnologie GmbH), 99947 Bad Langensalza, GermanyChemical and Veterinary and Investigations Office (Chemisches und Veterinäruntersuchungsamt), 70702 Fellbach, GermanyChemical and Veterinary and Investigations Office (Chemisches und Veterinäruntersuchungsamt), 70702 Fellbach, GermanyInstitute of Animal Hygiene and Veterinary Public Health, University of Leipzig, 04103 Leipzig, GermanyFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, 07743 Jena, GermanyFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, 07743 Jena, Germany<i>Coxiella burnetii</i> is the causative agent of Q fever, a zoonosis infecting domestic ruminants and humans. Currently used routine diagnostic tools offer limited sensitivity and specificity and symptomless infected animals may be missed. Therefore, diagnostic tools of higher sensitivity and specificity must be developed. For this purpose, the <i>C. burnetii</i> outer membrane protein Com1 was cloned and expressed in <i>Escherichia coli</i>. The His-tagged recombinant protein was purified and used in an indirect enzyme-linked immunosorbent assay (ELISA). Assay performance was tested with more than 400 positive and negative sera from sheep, goats and cattle from 36 locations. Calculation of sensitivity and specificity was undertaken using receiver operating characteristic (ROC) curves. The sensitivities and specificities for sheep were 85% and 68% (optical density at 450nm, OD<sub>450</sub> cut-off value 0.32), for goats 94% and 77% (OD<sub>450</sub> cut-off value 0.23) and for cattle 71% and 70% (OD<sub>450</sub> cut-off value 0.18), respectively. These results correspond to excellent, outstanding and acceptable discrimination of positive and negative sera. In summary, recombinant Com1 can provide a basis for more sensitive and specific diagnostic tools in veterinary medicine.https://www.mdpi.com/2076-2607/8/8/1235<i>C. burnetii</i>Q feverCom1antigenELISAdiagnostic |
| spellingShingle | Mareike Stellfeld Claudia Gerlach Ina-Gabriele Richter Peter Miethe Dominika Fahlbusch Birgitta Polley Reinhard Sting Martin Pfeffer Heinrich Neubauer Katja Mertens-Scholz Evaluation of the Diagnostic Potential of Recombinant <i>Coxiella burnetii</i> Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle Microorganisms <i>C. burnetii</i> Q fever Com1 antigen ELISA diagnostic |
| title | Evaluation of the Diagnostic Potential of Recombinant <i>Coxiella burnetii</i> Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
| title_full | Evaluation of the Diagnostic Potential of Recombinant <i>Coxiella burnetii</i> Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
| title_fullStr | Evaluation of the Diagnostic Potential of Recombinant <i>Coxiella burnetii</i> Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
| title_full_unstemmed | Evaluation of the Diagnostic Potential of Recombinant <i>Coxiella burnetii</i> Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
| title_short | Evaluation of the Diagnostic Potential of Recombinant <i>Coxiella burnetii</i> Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
| title_sort | evaluation of the diagnostic potential of recombinant i coxiella burnetii i com1 in an elisa for the diagnosis of q fever in sheep goats and cattle |
| topic | <i>C. burnetii</i> Q fever Com1 antigen ELISA diagnostic |
| url | https://www.mdpi.com/2076-2607/8/8/1235 |
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