The complete mitogenome of Argas vulgaris (Filippova, 1961) and its phylogenetic status in subgenus Argas (Acari: Argasidae)
Soft ticks (Ixodida: Argasidae) are ectoparasites of terrestrial vertebrates with worldwide distributions. As one representative group of Argasidae, the genus Argas has an important vectorial role in transmitting zoonotic diseases. However, our knowledge of the subgenus Argas in China is still limit...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | International Journal for Parasitology: Parasites and Wildlife |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224424000087 |
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| author | Yi-Fei Wang Jia-Jing Zheng Ming-Zhu Zhang Li-Feng Du Xiao-Ming Cui Xiao-Yu Han Di Tian Nuo Cheng Ning Wang Wan-Ying Gao Bai-Hui Wang Xiao-Yu Shi Jia-Fu Jiang Na Jia Yi Sun Wenqiang Shi Wu-Chun Cao |
| author_facet | Yi-Fei Wang Jia-Jing Zheng Ming-Zhu Zhang Li-Feng Du Xiao-Ming Cui Xiao-Yu Han Di Tian Nuo Cheng Ning Wang Wan-Ying Gao Bai-Hui Wang Xiao-Yu Shi Jia-Fu Jiang Na Jia Yi Sun Wenqiang Shi Wu-Chun Cao |
| author_sort | Yi-Fei Wang |
| collection | DOAJ |
| description | Soft ticks (Ixodida: Argasidae) are ectoparasites of terrestrial vertebrates with worldwide distributions. As one representative group of Argasidae, the genus Argas has an important vectorial role in transmitting zoonotic diseases. However, our knowledge of the subgenus Argas in China is still limited, as most literature only lists occurrence records or describes specific case reports without providing detailed morphological characteristics and further molecular data. This study aims to characterize Argas vulgaris through complete mitochondrial sequencing and morphological diagnostic techniques based on a batch of adult specimens collected from Ningxia Hui Autonomous Regions (NXHAR), North China. The morphology and microstructures of Ar. vulgaris and other lectotypes of argasid ticks in the subgenus Argas were also observed using a stereomicroscope. Following DNA extraction and sequencing, a complete mitochondrial sequence of Ar. vulgaris was assembled and analyzed within a phylogenetic context. The 14,479 bp mitogenome of Ar. vulgaris consists of 37 genes, including 13 genes for protein coding, two for ribosomal RNA, 22 for transfer RNA, and one for control region (D-loops). Phylogenetic analysis of Ar. vulgaris showed 98.27%–100% nucleotide identity with Ar. japonicus, indicating a close relationship between the two tick species. The morphological diagnostic features to differentiate Ar. vulgaris from other ticks within the subgenus Argas included the location of the anus and setae on the anterior lip of the female genital aperture. This study provided high-resolution scanning electron microscope images of female Ar. vulgaris and corresponding molecular data, representing valuable resources for future accurate species identification. |
| first_indexed | 2024-03-08T02:01:46Z |
| format | Article |
| id | doaj.art-a13c82ea71364a4eae9c2d31f9985e23 |
| institution | Directory Open Access Journal |
| issn | 2213-2244 |
| language | English |
| last_indexed | 2024-04-24T13:10:52Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj.art-a13c82ea71364a4eae9c2d31f9985e232024-04-05T04:40:38ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442024-04-0123100912The complete mitogenome of Argas vulgaris (Filippova, 1961) and its phylogenetic status in subgenus Argas (Acari: Argasidae)Yi-Fei Wang0Jia-Jing Zheng1Ming-Zhu Zhang2Li-Feng Du3Xiao-Ming Cui4Xiao-Yu Han5Di Tian6Nuo Cheng7Ning Wang8Wan-Ying Gao9Bai-Hui Wang10Xiao-Yu Shi11Jia-Fu Jiang12Na Jia13Yi Sun14Wenqiang Shi15Wu-Chun Cao16Institute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China; School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaSchool of Public Health, Ningxia Medical University, Yinchuan 750004, Ningxia, ChinaInstitute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102600, ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China; Corresponding author.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China; Corresponding author.Institute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China; Corresponding author. Institute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China.Soft ticks (Ixodida: Argasidae) are ectoparasites of terrestrial vertebrates with worldwide distributions. As one representative group of Argasidae, the genus Argas has an important vectorial role in transmitting zoonotic diseases. However, our knowledge of the subgenus Argas in China is still limited, as most literature only lists occurrence records or describes specific case reports without providing detailed morphological characteristics and further molecular data. This study aims to characterize Argas vulgaris through complete mitochondrial sequencing and morphological diagnostic techniques based on a batch of adult specimens collected from Ningxia Hui Autonomous Regions (NXHAR), North China. The morphology and microstructures of Ar. vulgaris and other lectotypes of argasid ticks in the subgenus Argas were also observed using a stereomicroscope. Following DNA extraction and sequencing, a complete mitochondrial sequence of Ar. vulgaris was assembled and analyzed within a phylogenetic context. The 14,479 bp mitogenome of Ar. vulgaris consists of 37 genes, including 13 genes for protein coding, two for ribosomal RNA, 22 for transfer RNA, and one for control region (D-loops). Phylogenetic analysis of Ar. vulgaris showed 98.27%–100% nucleotide identity with Ar. japonicus, indicating a close relationship between the two tick species. The morphological diagnostic features to differentiate Ar. vulgaris from other ticks within the subgenus Argas included the location of the anus and setae on the anterior lip of the female genital aperture. This study provided high-resolution scanning electron microscope images of female Ar. vulgaris and corresponding molecular data, representing valuable resources for future accurate species identification.http://www.sciencedirect.com/science/article/pii/S2213224424000087ArgasMorphologicalMitochondrial genomePhylogenetic |
| spellingShingle | Yi-Fei Wang Jia-Jing Zheng Ming-Zhu Zhang Li-Feng Du Xiao-Ming Cui Xiao-Yu Han Di Tian Nuo Cheng Ning Wang Wan-Ying Gao Bai-Hui Wang Xiao-Yu Shi Jia-Fu Jiang Na Jia Yi Sun Wenqiang Shi Wu-Chun Cao The complete mitogenome of Argas vulgaris (Filippova, 1961) and its phylogenetic status in subgenus Argas (Acari: Argasidae) International Journal for Parasitology: Parasites and Wildlife Argas Morphological Mitochondrial genome Phylogenetic |
| title | The complete mitogenome of Argas vulgaris (Filippova, 1961) and its phylogenetic status in subgenus Argas (Acari: Argasidae) |
| title_full | The complete mitogenome of Argas vulgaris (Filippova, 1961) and its phylogenetic status in subgenus Argas (Acari: Argasidae) |
| title_fullStr | The complete mitogenome of Argas vulgaris (Filippova, 1961) and its phylogenetic status in subgenus Argas (Acari: Argasidae) |
| title_full_unstemmed | The complete mitogenome of Argas vulgaris (Filippova, 1961) and its phylogenetic status in subgenus Argas (Acari: Argasidae) |
| title_short | The complete mitogenome of Argas vulgaris (Filippova, 1961) and its phylogenetic status in subgenus Argas (Acari: Argasidae) |
| title_sort | complete mitogenome of argas vulgaris filippova 1961 and its phylogenetic status in subgenus argas acari argasidae |
| topic | Argas Morphological Mitochondrial genome Phylogenetic |
| url | http://www.sciencedirect.com/science/article/pii/S2213224424000087 |
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