Evaluation of dynamic change in regenerated tendons in a mouse model
Abstract Background Using the film model method, the process whereby a substance called tendon gel is secreted from transected tendon ends and changed into a tendon after application of a traction force is known. The objective of this study was to investigate the association between mechanical prope...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-09-01
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| Series: | Journal of Experimental Orthopaedics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40634-018-0152-6 |
| _version_ | 1797330353733500928 |
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| author | Yoshinori Ohashi Junsuke Nakase Kengo Shimozaki Kojun Torigoe Hiroyuki Tsuchiya |
| author_facet | Yoshinori Ohashi Junsuke Nakase Kengo Shimozaki Kojun Torigoe Hiroyuki Tsuchiya |
| author_sort | Yoshinori Ohashi |
| collection | DOAJ |
| description | Abstract Background Using the film model method, the process whereby a substance called tendon gel is secreted from transected tendon ends and changed into a tendon after application of a traction force is known. The objective of this study was to investigate the association between mechanical properties in the early stages of tendon regeneration and time by using the film model method. Method Adult male ddY mice, closed colony mice established and maintained in Japan, were prepared for each experimental group. The study animals were 30 mice and were divided into three groups of 10 mice each. Ten specimens of tendon gel secreted from the transected tendon ends were collected on days 10, 15, and 20 postoperatively. While a traction force of 0.00245 N was applied to these specimens, the process of tendon gel changing into a tendon was video recorded for 24 h, and the length of extension was measured over time. Regenerated tendons were stained with hematoxylin and eosin for histological examination. Healing site was studied histologically according to the our maturity score with reference to the Bonar’s scale. Results The day 10 specimens gradually stretched for 12 h after the start of pulling and transformed into tendons. In contrast, the day 15 and 20 specimens stretched immediately after the start of pulling and transformed into tendons. The day 10 specimens stretched significantly more than the day 15 and 20 specimens (mechanical strain; 0.43 ± 0.26%, 0.03 ± 0.02%, and 0.03 ± 0.01%, respectively)Statistically significant differences were observed in the day 10 specimens than in the day 15 and 20 specimens. (P < 0.017). Using our maturity scores, the day 15 and 20 specimens were more mature than the day 10 specimens. (1.6 ± 0.68, 3.9 ± 0.54, and 4.8 ± 0.64, respectively) Statistically significant differences were observed in the day 10 specimens than in the day 15 and 20 specimens (P < 0.017). Conclusion Tendon gel physiologically and histologically matures on or after day 15 and becomes stronger dynamically in mechanical strength after day 15 than after day 10. |
| first_indexed | 2024-03-08T07:18:41Z |
| format | Article |
| id | doaj.art-a13fdd9641ed44219703eaca6e4df16b |
| institution | Directory Open Access Journal |
| issn | 2197-1153 |
| language | English |
| last_indexed | 2024-03-08T07:18:41Z |
| publishDate | 2018-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Experimental Orthopaedics |
| spelling | doaj.art-a13fdd9641ed44219703eaca6e4df16b2024-02-03T00:07:02ZengSpringerOpenJournal of Experimental Orthopaedics2197-11532018-09-01511810.1186/s40634-018-0152-6Evaluation of dynamic change in regenerated tendons in a mouse modelYoshinori Ohashi0Junsuke Nakase1Kengo Shimozaki2Kojun Torigoe3Hiroyuki Tsuchiya4Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kanazawa UniversityDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kanazawa UniversityDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kanazawa UniversityDepartment of Rehabilitation, Fukui Health Science University Faculty of Health ScienceDepartment of Orthopaedic Surgery, Graduate School of Medical Sciences, Kanazawa UniversityAbstract Background Using the film model method, the process whereby a substance called tendon gel is secreted from transected tendon ends and changed into a tendon after application of a traction force is known. The objective of this study was to investigate the association between mechanical properties in the early stages of tendon regeneration and time by using the film model method. Method Adult male ddY mice, closed colony mice established and maintained in Japan, were prepared for each experimental group. The study animals were 30 mice and were divided into three groups of 10 mice each. Ten specimens of tendon gel secreted from the transected tendon ends were collected on days 10, 15, and 20 postoperatively. While a traction force of 0.00245 N was applied to these specimens, the process of tendon gel changing into a tendon was video recorded for 24 h, and the length of extension was measured over time. Regenerated tendons were stained with hematoxylin and eosin for histological examination. Healing site was studied histologically according to the our maturity score with reference to the Bonar’s scale. Results The day 10 specimens gradually stretched for 12 h after the start of pulling and transformed into tendons. In contrast, the day 15 and 20 specimens stretched immediately after the start of pulling and transformed into tendons. The day 10 specimens stretched significantly more than the day 15 and 20 specimens (mechanical strain; 0.43 ± 0.26%, 0.03 ± 0.02%, and 0.03 ± 0.01%, respectively)Statistically significant differences were observed in the day 10 specimens than in the day 15 and 20 specimens. (P < 0.017). Using our maturity scores, the day 15 and 20 specimens were more mature than the day 10 specimens. (1.6 ± 0.68, 3.9 ± 0.54, and 4.8 ± 0.64, respectively) Statistically significant differences were observed in the day 10 specimens than in the day 15 and 20 specimens (P < 0.017). Conclusion Tendon gel physiologically and histologically matures on or after day 15 and becomes stronger dynamically in mechanical strength after day 15 than after day 10.http://link.springer.com/article/10.1186/s40634-018-0152-6Tendon gelTendon regenerationFilm model methodIntrinsic healingTraction force |
| spellingShingle | Yoshinori Ohashi Junsuke Nakase Kengo Shimozaki Kojun Torigoe Hiroyuki Tsuchiya Evaluation of dynamic change in regenerated tendons in a mouse model Journal of Experimental Orthopaedics Tendon gel Tendon regeneration Film model method Intrinsic healing Traction force |
| title | Evaluation of dynamic change in regenerated tendons in a mouse model |
| title_full | Evaluation of dynamic change in regenerated tendons in a mouse model |
| title_fullStr | Evaluation of dynamic change in regenerated tendons in a mouse model |
| title_full_unstemmed | Evaluation of dynamic change in regenerated tendons in a mouse model |
| title_short | Evaluation of dynamic change in regenerated tendons in a mouse model |
| title_sort | evaluation of dynamic change in regenerated tendons in a mouse model |
| topic | Tendon gel Tendon regeneration Film model method Intrinsic healing Traction force |
| url | http://link.springer.com/article/10.1186/s40634-018-0152-6 |
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