Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance
This study was intended to obtain white rust (Puccinia horiana) disease resistance Chrysanthemum transformed with wasabi defensin gene through mediation of Agrobacterium tumefaciens from three explant sources, i.e., leaf, lateral shoot bud, and internode. Observations were made on transformation ef...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2022-05-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/38122 |
| _version_ | 1811228860181643264 |
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| author | Rinaldi Sjahril Irma Jamaluddin Marhamah Nadir Asman Asman Feranita Haring Muhammad Riadi Siti Halimah Larekeng Totik Sri Mariani Trisnawaty A.R Nurhaya J. Panga Astina Tambung Dong Poh Chin Masahiro Mii |
| author_facet | Rinaldi Sjahril Irma Jamaluddin Marhamah Nadir Asman Asman Feranita Haring Muhammad Riadi Siti Halimah Larekeng Totik Sri Mariani Trisnawaty A.R Nurhaya J. Panga Astina Tambung Dong Poh Chin Masahiro Mii |
| author_sort | Rinaldi Sjahril |
| collection | DOAJ |
| description |
This study was intended to obtain white rust (Puccinia horiana) disease resistance Chrysanthemum transformed with wasabi defensin gene through mediation of Agrobacterium tumefaciens from three explant sources, i.e., leaf, lateral shoot bud, and internode. Observations were made on transformation efficiency, PCR analysis, in vitro and ex vitro disease resistance tests. Results showed that efficiency of transgenic callus and shoot regeneration was found both highest from lateral shoot buds (57.5% and 50.0%, respectively). PCR analysis showed that three putative transgenic plantlets from lateral shoot buds and one from leaf explant were putative transgenic carrying the wasabi, hpt, and nptII genes. Rooting test showed that the highest number of rooted plants was found in treatment of hygromycin (Hg) 25 mg L-1 (81%) and lowest was in treatment combination of kanamycin (Km) 50 mg L-1 + Hg 25 mg L-1 (25%). In vitro disease resistance test with sorus inoculation of P. horiana, directly on the leaves, resulted in 20 resistant plants out of 30 putative transgenic plants (66.67%). Ex vitro testing on adult plants of the same samples in a confined closed greenhouse (CGH) resulted in average of 80% transgenic Chrysanthemum plants were resistant, whereas in control plants caused white rust disease symptom.
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| first_indexed | 2024-04-12T10:04:30Z |
| format | Article |
| id | doaj.art-a140f94a891a4969b024d8a60e6496cd |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-04-12T10:04:30Z |
| publishDate | 2022-05-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-a140f94a891a4969b024d8a60e6496cd2022-12-22T03:37:29ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942022-05-0129510.4308/hjb.29.5.621-631Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease ResistanceRinaldi Sjahril0Irma Jamaluddin1Marhamah Nadir2Asman Asman3Feranita Haring4Muhammad Riadi5Siti Halimah Larekeng6Totik Sri Mariani7Trisnawaty A.R8Nurhaya J. Panga9Astina Tambung10Dong Poh Chin11Masahiro Mii12Laboratory of Plant Biosciences and Reproduction Biotechnology, Faculty of Agriculture, Universitas Hasanuddin Makassar, IndonesiaMaster Student, Agrotechnology, Faculty of Agriculture, Universitas Hasanuddin, Makassar, IndonesiaAnimal Feed Chemistry Laboratory, Faculty of Animal Husbandry, Universitas Hasanuddin, Makassar, IndonesiaPlant Pest and Disease Laboratory, Department of Plant Pest and Disease Science, Faculty of Agriculture, Universitas Hasanuddin, Makassar, IndonesiaLaboratory of Plant Biosciences and Reproduction Biotechnology, Faculty of Agriculture, Universitas Hasanuddin Makassar, IndonesiaPlant Breeding and Seed Sciences Laboratory, Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin, Makassar, IndonesiaBiotechnology and Tree Breeding Laboratory, Department of Forestry, Faculty of Forestry, Universitas Hasanuddin, Makassar, IndonesiaSchool of Life Science and Technology, Bandung Institute of Technology, Bandung, IndonesiaDepartment of Agrotechnology, Faculty of Sciences and Technology, University of Muhammadiyah Sidenreng Rappang, Sidenreng Rappang, Indonesia Faculty of Agriculture, Musamus University, Merauke, Papua, IndonesiaLaboratory of Plant Biosciences and Reproduction Biotechnology, Faculty of Agriculture, Universitas Hasanuddin Makassar, IndonesiaGraduate School of Horticulture, Chiba University, Matsudo, Chiba, JapanGraduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan This study was intended to obtain white rust (Puccinia horiana) disease resistance Chrysanthemum transformed with wasabi defensin gene through mediation of Agrobacterium tumefaciens from three explant sources, i.e., leaf, lateral shoot bud, and internode. Observations were made on transformation efficiency, PCR analysis, in vitro and ex vitro disease resistance tests. Results showed that efficiency of transgenic callus and shoot regeneration was found both highest from lateral shoot buds (57.5% and 50.0%, respectively). PCR analysis showed that three putative transgenic plantlets from lateral shoot buds and one from leaf explant were putative transgenic carrying the wasabi, hpt, and nptII genes. Rooting test showed that the highest number of rooted plants was found in treatment of hygromycin (Hg) 25 mg L-1 (81%) and lowest was in treatment combination of kanamycin (Km) 50 mg L-1 + Hg 25 mg L-1 (25%). In vitro disease resistance test with sorus inoculation of P. horiana, directly on the leaves, resulted in 20 resistant plants out of 30 putative transgenic plants (66.67%). Ex vitro testing on adult plants of the same samples in a confined closed greenhouse (CGH) resulted in average of 80% transgenic Chrysanthemum plants were resistant, whereas in control plants caused white rust disease symptom. https://journal.ipb.ac.id/index.php/hayati/article/view/38122 |
| spellingShingle | Rinaldi Sjahril Irma Jamaluddin Marhamah Nadir Asman Asman Feranita Haring Muhammad Riadi Siti Halimah Larekeng Totik Sri Mariani Trisnawaty A.R Nurhaya J. Panga Astina Tambung Dong Poh Chin Masahiro Mii Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance Hayati Journal of Biosciences |
| title | Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance |
| title_full | Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance |
| title_fullStr | Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance |
| title_full_unstemmed | Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance |
| title_short | Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance |
| title_sort | performance of transgenic chrysanthemum harbouring wasabi defensin gene for white rust disease resistance |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/38122 |
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