Transmission of blaNDM in Enterobacteriaceae among animals, food and human
ABSTRACTDespite carbapenems not being used in animals, carbapenem-resistant Enterobacterales (CRE), particularly New Delhi metallo-β-lactamase-producing CRE (NDM-CRE), are prevalent in livestock. Concurrently, the incidence of human infections caused by NDM-CRE is rising, particularly in children. A...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2024.2337678 |
| _version_ | 1797199926470377472 |
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| author | Bo Fu Jian Xu Dandan Yin Chengtao Sun Dejun Liu Weishuai Zhai Rina Bai Yue Cao Qin Zhang Shizhen Ma Timothy R. Walsh Fupin Hu Yang Wang Congming Wu Jianzhong Shen |
| author_facet | Bo Fu Jian Xu Dandan Yin Chengtao Sun Dejun Liu Weishuai Zhai Rina Bai Yue Cao Qin Zhang Shizhen Ma Timothy R. Walsh Fupin Hu Yang Wang Congming Wu Jianzhong Shen |
| author_sort | Bo Fu |
| collection | DOAJ |
| description | ABSTRACTDespite carbapenems not being used in animals, carbapenem-resistant Enterobacterales (CRE), particularly New Delhi metallo-β-lactamase-producing CRE (NDM-CRE), are prevalent in livestock. Concurrently, the incidence of human infections caused by NDM-CRE is rising, particularly in children. Although a positive association between livestock production and human NDM-CRE infections at the national level was identified, the evidence of direct transmission of NDM originating from livestock to humans remains largely unknown. Here, we conducted a cross-sectional study in Chengdu, Sichuan Province, to examine the prevalence of NDM-CRE in chickens and pigs along the breeding–slaughtering–retail chains, in pork in cafeterias of schools, and in colonizations and infections from children's hospital and examined the correlation of NDM-CRE among animals, foods and humans. Overall, the blaNDM increases gradually along the chicken and pig breeding (4.70%/2.0%) –slaughtering (7.60%/22.40%) –retail (65.56%/34.26%) chains. The slaughterhouse has become a hotspot for cross-contamination and amplifier of blaNDM. Notably, 63.11% of pork from the school cafeteria was positive for blaNDM. The prevalence of blaNDM in intestinal and infection samples from children's hospitals was 21.68% and 19.80%, respectively. whole genome sequencing (WGS) analysis revealed the sporadic, not large-scale, clonal spread of NDM-CRE along the chicken and pig breeding–slaughtering–retail chain, with further spreading via IncX3-blaNDM plasmid within each stage of whole chains. Clonal transmission of NDM-CRE is predominant in children's hospitals. The IncX3-blaNDM plasmid was highly prevalent among animals and humans and accounted for 57.7% of Escherichia coli and 91.3% of Klebsiella pneumoniae. Attention should be directed towards the IncX3 plasmid to control the transmission of blaNDM between animals and humans. |
| first_indexed | 2024-04-24T07:23:31Z |
| format | Article |
| id | doaj.art-a141bb1d016f458994a2d9acbb3f7ea2 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-24T07:23:31Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-a141bb1d016f458994a2d9acbb3f7ea22024-04-20T19:55:59ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512024-12-0113110.1080/22221751.2024.2337678Transmission of blaNDM in Enterobacteriaceae among animals, food and humanBo Fu0Jian Xu1Dandan Yin2Chengtao Sun3Dejun Liu4Weishuai Zhai5Rina Bai6Yue Cao7Qin Zhang8Shizhen Ma9Timothy R. Walsh10Fupin Hu11Yang Wang12Congming Wu13Jianzhong Shen14National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaChengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, People’s Republic of ChinaInstitute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaChengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaInstitute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People’s Republic of ChinaABSTRACTDespite carbapenems not being used in animals, carbapenem-resistant Enterobacterales (CRE), particularly New Delhi metallo-β-lactamase-producing CRE (NDM-CRE), are prevalent in livestock. Concurrently, the incidence of human infections caused by NDM-CRE is rising, particularly in children. Although a positive association between livestock production and human NDM-CRE infections at the national level was identified, the evidence of direct transmission of NDM originating from livestock to humans remains largely unknown. Here, we conducted a cross-sectional study in Chengdu, Sichuan Province, to examine the prevalence of NDM-CRE in chickens and pigs along the breeding–slaughtering–retail chains, in pork in cafeterias of schools, and in colonizations and infections from children's hospital and examined the correlation of NDM-CRE among animals, foods and humans. Overall, the blaNDM increases gradually along the chicken and pig breeding (4.70%/2.0%) –slaughtering (7.60%/22.40%) –retail (65.56%/34.26%) chains. The slaughterhouse has become a hotspot for cross-contamination and amplifier of blaNDM. Notably, 63.11% of pork from the school cafeteria was positive for blaNDM. The prevalence of blaNDM in intestinal and infection samples from children's hospitals was 21.68% and 19.80%, respectively. whole genome sequencing (WGS) analysis revealed the sporadic, not large-scale, clonal spread of NDM-CRE along the chicken and pig breeding–slaughtering–retail chain, with further spreading via IncX3-blaNDM plasmid within each stage of whole chains. Clonal transmission of NDM-CRE is predominant in children's hospitals. The IncX3-blaNDM plasmid was highly prevalent among animals and humans and accounted for 57.7% of Escherichia coli and 91.3% of Klebsiella pneumoniae. Attention should be directed towards the IncX3 plasmid to control the transmission of blaNDM between animals and humans.https://www.tandfonline.com/doi/10.1080/22221751.2024.2337678NDM-CREfood chainIncX3livestockchild |
| spellingShingle | Bo Fu Jian Xu Dandan Yin Chengtao Sun Dejun Liu Weishuai Zhai Rina Bai Yue Cao Qin Zhang Shizhen Ma Timothy R. Walsh Fupin Hu Yang Wang Congming Wu Jianzhong Shen Transmission of blaNDM in Enterobacteriaceae among animals, food and human Emerging Microbes and Infections NDM-CRE food chain IncX3 livestock child |
| title | Transmission of blaNDM in Enterobacteriaceae among animals, food and human |
| title_full | Transmission of blaNDM in Enterobacteriaceae among animals, food and human |
| title_fullStr | Transmission of blaNDM in Enterobacteriaceae among animals, food and human |
| title_full_unstemmed | Transmission of blaNDM in Enterobacteriaceae among animals, food and human |
| title_short | Transmission of blaNDM in Enterobacteriaceae among animals, food and human |
| title_sort | transmission of blandm in enterobacteriaceae among animals food and human |
| topic | NDM-CRE food chain IncX3 livestock child |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2024.2337678 |
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