Purification and Immobilization of the Recombinant Brassica oleracea Chlorophyllase 1 (BoCLH1) on DIAION®CR11 as Potential Biocatalyst for the Production of Chlorophyllide and Phytol

Purification and Immobilization of the Recombinant Brassica oleracea Chlorophyllase 1 (BoCLH1) on DIAION®CR11 as Potential Biocatalyst for the Production of Chlorophyllide and Phytol

Recombinant Brassica oleracea chlorophyllase 1 (BoCLH1) with a protein molecular weight of 38.63 kDa was successfully expressed in E. coli and could catalyze chlorophyll (Chl) hydrolysis to chlorophyllide and phytol in vitro. In this study, we used DIAION®CR11, a highly porous cross-linked polystyre...

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Bibliographic Details
Main Authors: Yi-Li Chou, Chia-Yun Ko, Long-Fang O. Chen, Chih-Chung Yen, Jei-Fu Shaw
Format: Article
Language:English
Published: MDPI AG 2015-02-01
Series:Molecules
Subjects:
enzyme purification
enzyme immobilization
Brassica oleracea chlorophyllase 1 (BoCLH1)
DIAION®CR11
Online Access:http://www.mdpi.com/1420-3049/20/3/3744

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