Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells
In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characteriz...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/9/11/1632 |
| _version_ | 1797511157955690496 |
|---|---|
| author | Chiung-Chyi Shen Shan-hui Hsu Kai-Bo Chang Chun-An Yeh Hsiang-Chun Chang Cheng-Ming Tang Yi-Chin Yang Hsien-Hsu Hsieh Huey-Shan Hung |
| author_facet | Chiung-Chyi Shen Shan-hui Hsu Kai-Bo Chang Chun-An Yeh Hsiang-Chun Chang Cheng-Ming Tang Yi-Chin Yang Hsien-Hsu Hsieh Huey-Shan Hung |
| author_sort | Chiung-Chyi Shen |
| collection | DOAJ |
| description | In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV–Vis spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and contact angle assessment. The effects of PEG-HA-AuNP nanocomposites on the biocompatibility and biological activity of MC3T3-E1 osteoblast cells, endothelial cells (EC), macrophages (RAW 264.7), and human mesenchymal stem cells (MSCs), as well as the guiding of osteogenic differentiation, were estimated through the use of an in vitro assay. Moreover, the anti-inflammatory, biocompatibility, and endothelialization capacities were further assessed through in vivo evaluation. The PEG-HA-AuNP nanocomposites showed superior biological properties and biocompatibility capacity for cell behavior in both MC3T3-E1 cells and MSCs. These biological events surrounding the cells could be associated with the activation of adhesion, proliferation, migration, and differentiation processes on the PEG-HA-AuNP nanocomposites. Indeed, the induction of the osteogenic differentiation of MSCs by PEG-HA-AuNP nanocomposites and enhanced mineralization activity were also evidenced in this study. Moreover, from the in vivo assay, we further found that PEG-HA-AuNP nanocomposites not only facilitate the anti-immune response, as well as reducing CD86 expression, but also facilitate the endothelialization ability, as well as promoting CD31 expression, when implanted into rats subcutaneously for a period of 1 month. The current research illustrates the potential of PEG-HA-AuNP nanocomposites when used in combination with MSCs for the regeneration of bone tissue, with their nanotopography being employed as an applicable surface modification approach for the fabrication of biomaterials. |
| first_indexed | 2024-03-10T05:41:29Z |
| format | Article |
| id | doaj.art-a14a46415f944a958b206f1ac8bbc418 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T05:41:29Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-a14a46415f944a958b206f1ac8bbc4182023-11-22T22:31:07ZengMDPI AGBiomedicines2227-90592021-11-01911163210.3390/biomedicines9111632Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem CellsChiung-Chyi Shen0Shan-hui Hsu1Kai-Bo Chang2Chun-An Yeh3Hsiang-Chun Chang4Cheng-Ming Tang5Yi-Chin Yang6Hsien-Hsu Hsieh7Huey-Shan Hung8Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407204, TaiwanInstitute of Polymer Science and Engineering, National Taiwan University, Taipei 10617, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 40402, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 40402, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 40402, TaiwanCollege of Oral Medicine, Chung Shan Medical University, Taichung 40201, TaiwanDepartment of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407204, TaiwanBlood Bank, Taichung Veterans General Hospital, Taichung 407204, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 40402, TaiwanIn this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV–Vis spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and contact angle assessment. The effects of PEG-HA-AuNP nanocomposites on the biocompatibility and biological activity of MC3T3-E1 osteoblast cells, endothelial cells (EC), macrophages (RAW 264.7), and human mesenchymal stem cells (MSCs), as well as the guiding of osteogenic differentiation, were estimated through the use of an in vitro assay. Moreover, the anti-inflammatory, biocompatibility, and endothelialization capacities were further assessed through in vivo evaluation. The PEG-HA-AuNP nanocomposites showed superior biological properties and biocompatibility capacity for cell behavior in both MC3T3-E1 cells and MSCs. These biological events surrounding the cells could be associated with the activation of adhesion, proliferation, migration, and differentiation processes on the PEG-HA-AuNP nanocomposites. Indeed, the induction of the osteogenic differentiation of MSCs by PEG-HA-AuNP nanocomposites and enhanced mineralization activity were also evidenced in this study. Moreover, from the in vivo assay, we further found that PEG-HA-AuNP nanocomposites not only facilitate the anti-immune response, as well as reducing CD86 expression, but also facilitate the endothelialization ability, as well as promoting CD31 expression, when implanted into rats subcutaneously for a period of 1 month. The current research illustrates the potential of PEG-HA-AuNP nanocomposites when used in combination with MSCs for the regeneration of bone tissue, with their nanotopography being employed as an applicable surface modification approach for the fabrication of biomaterials.https://www.mdpi.com/2227-9059/9/11/1632polyethylene glycolhydroxyapatitephysical gold nanoparticlemesenchymal stem cells |
| spellingShingle | Chiung-Chyi Shen Shan-hui Hsu Kai-Bo Chang Chun-An Yeh Hsiang-Chun Chang Cheng-Ming Tang Yi-Chin Yang Hsien-Hsu Hsieh Huey-Shan Hung Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells Biomedicines polyethylene glycol hydroxyapatite physical gold nanoparticle mesenchymal stem cells |
| title | Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells |
| title_full | Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells |
| title_fullStr | Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells |
| title_full_unstemmed | Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells |
| title_short | Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells |
| title_sort | physical gold nanoparticle decorated polyethylene glycol hydroxyapatite composites guide osteogenesis and angiogenesis of mesenchymal stem cells |
| topic | polyethylene glycol hydroxyapatite physical gold nanoparticle mesenchymal stem cells |
| url | https://www.mdpi.com/2227-9059/9/11/1632 |
| work_keys_str_mv | AT chiungchyishen physicalgoldnanoparticledecoratedpolyethyleneglycolhydroxyapatitecompositesguideosteogenesisandangiogenesisofmesenchymalstemcells AT shanhuihsu physicalgoldnanoparticledecoratedpolyethyleneglycolhydroxyapatitecompositesguideosteogenesisandangiogenesisofmesenchymalstemcells AT kaibochang physicalgoldnanoparticledecoratedpolyethyleneglycolhydroxyapatitecompositesguideosteogenesisandangiogenesisofmesenchymalstemcells AT chunanyeh physicalgoldnanoparticledecoratedpolyethyleneglycolhydroxyapatitecompositesguideosteogenesisandangiogenesisofmesenchymalstemcells AT hsiangchunchang physicalgoldnanoparticledecoratedpolyethyleneglycolhydroxyapatitecompositesguideosteogenesisandangiogenesisofmesenchymalstemcells AT chengmingtang physicalgoldnanoparticledecoratedpolyethyleneglycolhydroxyapatitecompositesguideosteogenesisandangiogenesisofmesenchymalstemcells AT yichinyang physicalgoldnanoparticledecoratedpolyethyleneglycolhydroxyapatitecompositesguideosteogenesisandangiogenesisofmesenchymalstemcells AT hsienhsuhsieh physicalgoldnanoparticledecoratedpolyethyleneglycolhydroxyapatitecompositesguideosteogenesisandangiogenesisofmesenchymalstemcells AT hueyshanhung physicalgoldnanoparticledecoratedpolyethyleneglycolhydroxyapatitecompositesguideosteogenesisandangiogenesisofmesenchymalstemcells |