In vitro evaluation of the anti-digestion and antioxidant effects of grape seed procyanidins according to their degrees of polymerization
Few studies have evaluated the anti-digestion effects of grape seed procyanidins (GSPs) compared to their strong anti-oxidant activities according to different degrees of polymerization (DPs). Additionally, the effects of GSPs with different DPs on the binding sites of digestive enzymes are unexplor...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-10-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618304201 |
| _version_ | 1818563882987290624 |
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| author | Peiyu Zhou Lingmin Zhang Wei Li Shuting Zhang Lanxin Luo Jian Wang Baoshan Sun |
| author_facet | Peiyu Zhou Lingmin Zhang Wei Li Shuting Zhang Lanxin Luo Jian Wang Baoshan Sun |
| author_sort | Peiyu Zhou |
| collection | DOAJ |
| description | Few studies have evaluated the anti-digestion effects of grape seed procyanidins (GSPs) compared to their strong anti-oxidant activities according to different degrees of polymerization (DPs). Additionally, the effects of GSPs with different DPs on the binding sites of digestive enzymes are unexplored. In this work, the anti-digestive and anti-oxidant activity of GSPs with different DPs were verified and compared. Anti-digestive activity assays were conducted on key digestive enzymes. Although all procyanidins fractions possessed antioxidant activity as expected, there was no significant difference in the antioxidant activity among procyanidins with different DPs. In contrast, the anti-digestive activity of the fractions increased significantly as the DP increased. Meanwhile, molecular docking provided a putative mechanism of anti-digestion, and GSPs can block the enzyme sites by hydrogen interactions, hydrophobic interactions and electrostatic interactions. These results indicated that the higher-molecular-weight procyanidins had equal or lower antioxidant activity and a greater anti-digestion effect than lower-molecular-weight procyanidins. |
| first_indexed | 2024-12-14T01:22:09Z |
| format | Article |
| id | doaj.art-a14a6b7903474323bb159be1ab692abe |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T01:22:09Z |
| publishDate | 2018-10-01 |
| publisher | Elsevier |
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| series | Journal of Functional Foods |
| spelling | doaj.art-a14a6b7903474323bb159be1ab692abe2022-12-21T23:22:21ZengElsevierJournal of Functional Foods1756-46462018-10-01498595In vitro evaluation of the anti-digestion and antioxidant effects of grape seed procyanidins according to their degrees of polymerizationPeiyu Zhou0Lingmin Zhang1Wei Li2Shuting Zhang3Lanxin Luo4Jian Wang5Baoshan Sun6School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang, PR ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 110016 Shenyang, PR ChinaDepartment of Pharmaceutical Engineering, Shenyang University of Chemical Technology, 110042 Shenyang, PR ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, 110016 Shenyang, PR ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 110016 Shenyang, PR ChinaKey Laboratory of Structure-Based Drug Design and Discovery, Shenyang Pharmaceutical University, Ministry of Education, 110016 Shenyang, PR China; Corresponding authors at: School of Functional Food and Wine, Shenyang Pharmaceutical University, 110016 Shenyang, PR China (B. Sun). Key Laboratory of Structure-Based Drug Design and Discovery, Shenyang Pharmaceutical University, 110016 Shenyang, PR China (J. Wang).School of Functional Food and Wine, Shenyang Pharmaceutical University, 110016 Shenyang, PR China; Pólo Dois Portos, Instituto National de Investigação Agrária e Veterinária, I.P., Quinta da Almoinha, 2565-191 Dois Portos, Portugal; Corresponding authors at: School of Functional Food and Wine, Shenyang Pharmaceutical University, 110016 Shenyang, PR China (B. Sun). Key Laboratory of Structure-Based Drug Design and Discovery, Shenyang Pharmaceutical University, 110016 Shenyang, PR China (J. Wang).Few studies have evaluated the anti-digestion effects of grape seed procyanidins (GSPs) compared to their strong anti-oxidant activities according to different degrees of polymerization (DPs). Additionally, the effects of GSPs with different DPs on the binding sites of digestive enzymes are unexplored. In this work, the anti-digestive and anti-oxidant activity of GSPs with different DPs were verified and compared. Anti-digestive activity assays were conducted on key digestive enzymes. Although all procyanidins fractions possessed antioxidant activity as expected, there was no significant difference in the antioxidant activity among procyanidins with different DPs. In contrast, the anti-digestive activity of the fractions increased significantly as the DP increased. Meanwhile, molecular docking provided a putative mechanism of anti-digestion, and GSPs can block the enzyme sites by hydrogen interactions, hydrophobic interactions and electrostatic interactions. These results indicated that the higher-molecular-weight procyanidins had equal or lower antioxidant activity and a greater anti-digestion effect than lower-molecular-weight procyanidins.http://www.sciencedirect.com/science/article/pii/S1756464618304201Grape seed procyanidinsα-AmylaseLipasePepsinAntioxidant activityMolecular docking |
| spellingShingle | Peiyu Zhou Lingmin Zhang Wei Li Shuting Zhang Lanxin Luo Jian Wang Baoshan Sun In vitro evaluation of the anti-digestion and antioxidant effects of grape seed procyanidins according to their degrees of polymerization Journal of Functional Foods Grape seed procyanidins α-Amylase Lipase Pepsin Antioxidant activity Molecular docking |
| title | In vitro evaluation of the anti-digestion and antioxidant effects of grape seed procyanidins according to their degrees of polymerization |
| title_full | In vitro evaluation of the anti-digestion and antioxidant effects of grape seed procyanidins according to their degrees of polymerization |
| title_fullStr | In vitro evaluation of the anti-digestion and antioxidant effects of grape seed procyanidins according to their degrees of polymerization |
| title_full_unstemmed | In vitro evaluation of the anti-digestion and antioxidant effects of grape seed procyanidins according to their degrees of polymerization |
| title_short | In vitro evaluation of the anti-digestion and antioxidant effects of grape seed procyanidins according to their degrees of polymerization |
| title_sort | in vitro evaluation of the anti digestion and antioxidant effects of grape seed procyanidins according to their degrees of polymerization |
| topic | Grape seed procyanidins α-Amylase Lipase Pepsin Antioxidant activity Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618304201 |
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