Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice
Abstract Objective This study aimed to demonstrate the immunohistochemical changes associated with MMP-2 and type 1 collagen separately for the first time in the major salivary glands (the parotid, submaxillary, and sublingual glands) that occur with aging in mice. Material and Methods Fourteen...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2018-06-01
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100461&lng=en&tlng=en |
| _version_ | 1811337474297823232 |
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| author | Mehmet Kemal TUMER Mustafa CICEK |
| author_facet | Mehmet Kemal TUMER Mustafa CICEK |
| author_sort | Mehmet Kemal TUMER |
| collection | DOAJ |
| description | Abstract Objective This study aimed to demonstrate the immunohistochemical changes associated with MMP-2 and type 1 collagen separately for the first time in the major salivary glands (the parotid, submaxillary, and sublingual glands) that occur with aging in mice. Material and Methods Fourteen Balb/c white mice (50-80 g) were used in this study. The animals were divided into two equal groups. Group I consisted of young animals (2-month-old) (n=7) and Group II consisted of older animals (18-month-old) (n=7). After routine histological follow-ups, Hematoxylin-eosin (H&E), Masson’s Trichrome staining and immunohistochemical staining was performed for type I collagen and MMP-2. Results We observed that there were age-related decreases in the number of acinar cells, increase in eosinophilic zymogen granules in cells, collagen accumulation in fibrotic areas and dilatation in interlobular ducts. Also, while type I collagen and MMP-2 immunoreactivity were moderate in the salivary glands of the young mice, they were high in the salivary glands of the old mice (p=0.001). In the H-score assessment, MMP-2 immunoreactivity was lower at a significant level in young mice than in old mice (p=0.001). Conclusions This study showed that anatomical, physiological and morphological abnormalities occur in all three major salivary glands as a natural consequence of aging. |
| first_indexed | 2024-04-13T17:55:20Z |
| format | Article |
| id | doaj.art-a14a97431fb145b7a277e79646047480 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-04-13T17:55:20Z |
| publishDate | 2018-06-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-a14a97431fb145b7a277e796460474802022-12-22T02:36:31ZengUniversity of São PauloJournal of Applied Oral Science1678-77652018-06-0126010.1590/1678-7757-2017-0484S1678-77572018000100461Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric miceMehmet Kemal TUMERMustafa CICEKAbstract Objective This study aimed to demonstrate the immunohistochemical changes associated with MMP-2 and type 1 collagen separately for the first time in the major salivary glands (the parotid, submaxillary, and sublingual glands) that occur with aging in mice. Material and Methods Fourteen Balb/c white mice (50-80 g) were used in this study. The animals were divided into two equal groups. Group I consisted of young animals (2-month-old) (n=7) and Group II consisted of older animals (18-month-old) (n=7). After routine histological follow-ups, Hematoxylin-eosin (H&E), Masson’s Trichrome staining and immunohistochemical staining was performed for type I collagen and MMP-2. Results We observed that there were age-related decreases in the number of acinar cells, increase in eosinophilic zymogen granules in cells, collagen accumulation in fibrotic areas and dilatation in interlobular ducts. Also, while type I collagen and MMP-2 immunoreactivity were moderate in the salivary glands of the young mice, they were high in the salivary glands of the old mice (p=0.001). In the H-score assessment, MMP-2 immunoreactivity was lower at a significant level in young mice than in old mice (p=0.001). Conclusions This study showed that anatomical, physiological and morphological abnormalities occur in all three major salivary glands as a natural consequence of aging.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100461&lng=en&tlng=enParotid glandType 1 collagenSalivary glandsMatrix metalloproteinase 2Aging |
| spellingShingle | Mehmet Kemal TUMER Mustafa CICEK Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice Journal of Applied Oral Science Parotid gland Type 1 collagen Salivary glands Matrix metalloproteinase 2 Aging |
| title | Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice |
| title_full | Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice |
| title_fullStr | Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice |
| title_full_unstemmed | Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice |
| title_short | Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice |
| title_sort | differential immunohistochemical expression of type i collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice |
| topic | Parotid gland Type 1 collagen Salivary glands Matrix metalloproteinase 2 Aging |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100461&lng=en&tlng=en |
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