Genomics of Dwarfism in Italian Local Chicken Breeds
The identification of the dwarf phenotype in chicken is based on body weight, height, and shank length, leaving the differentiation between dwarf and small breeds ambiguous. The aims of the present study were to characterize the sequence variations associated with the dwarf phenotype in three Italia...
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MDPI AG
2023-03-01
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author | Francesco Perini Filippo Cendron Zhou Wu Natalia Sevane Zhiqiang Li Chunhua Huang Jacqueline Smith Emiliano Lasagna Martino Cassandro Mauro Penasa |
author_facet | Francesco Perini Filippo Cendron Zhou Wu Natalia Sevane Zhiqiang Li Chunhua Huang Jacqueline Smith Emiliano Lasagna Martino Cassandro Mauro Penasa |
author_sort | Francesco Perini |
collection | DOAJ |
description | The identification of the dwarf phenotype in chicken is based on body weight, height, and shank length, leaving the differentiation between dwarf and small breeds ambiguous. The aims of the present study were to characterize the sequence variations associated with the dwarf phenotype in three Italian chicken breeds and to investigate the genes associated with their phenotype. Five hundred and forty-one chickens from 23 local breeds (from 20 to 24 animals per breed) were sampled. All animals were genotyped with the 600 K chicken SNP array. Three breeds were described as “dwarf”, namely, Mericanel della Brianza (MERI), Mugellese (MUG), and Pepoi (PPP). We compared MERI, MUG, and PPP with the four heaviest breeds in the dataset by performing genome-wide association studies. Results showed significant SNPs associated with dwarfism in the MERI and MUG breeds, which shared a candidate genomic region on chromosome 1. Due to this similarity, MERI and MUG were analyzed together as a meta-population, observing significant SNPs in the <i>LEMD3</i> and <i>HMGA2</i> genes, which were previously reported as being responsible for dwarfism in different species. In conclusion, MERI and MUG breeds seem to share a genetic basis of dwarfism, which differentiates them from the small PPP breed. |
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issn | 2073-4425 |
language | English |
last_indexed | 2024-03-11T06:30:15Z |
publishDate | 2023-03-01 |
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series | Genes |
spelling | doaj.art-a156ec5d41b64650b5705fcf6c1baaed2023-11-17T11:17:18ZengMDPI AGGenes2073-44252023-03-0114363310.3390/genes14030633Genomics of Dwarfism in Italian Local Chicken BreedsFrancesco Perini0Filippo Cendron1Zhou Wu2Natalia Sevane3Zhiqiang Li4Chunhua Huang5Jacqueline Smith6Emiliano Lasagna7Martino Cassandro8Mauro Penasa9Department of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, 35020 Legnaro, ItalyThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UKDepartment of Animal Production, Veterinary Faculty, Universidad Complutense de Madrid, Avenida Puerta de Hierro, 28040 Madrid, SpainDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UKDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, 35020 Legnaro, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, 35020 Legnaro, ItalyThe identification of the dwarf phenotype in chicken is based on body weight, height, and shank length, leaving the differentiation between dwarf and small breeds ambiguous. The aims of the present study were to characterize the sequence variations associated with the dwarf phenotype in three Italian chicken breeds and to investigate the genes associated with their phenotype. Five hundred and forty-one chickens from 23 local breeds (from 20 to 24 animals per breed) were sampled. All animals were genotyped with the 600 K chicken SNP array. Three breeds were described as “dwarf”, namely, Mericanel della Brianza (MERI), Mugellese (MUG), and Pepoi (PPP). We compared MERI, MUG, and PPP with the four heaviest breeds in the dataset by performing genome-wide association studies. Results showed significant SNPs associated with dwarfism in the MERI and MUG breeds, which shared a candidate genomic region on chromosome 1. Due to this similarity, MERI and MUG were analyzed together as a meta-population, observing significant SNPs in the <i>LEMD3</i> and <i>HMGA2</i> genes, which were previously reported as being responsible for dwarfism in different species. In conclusion, MERI and MUG breeds seem to share a genetic basis of dwarfism, which differentiates them from the small PPP breed.https://www.mdpi.com/2073-4425/14/3/633genomic regionindigenous breedchickenSNPGWAS |
spellingShingle | Francesco Perini Filippo Cendron Zhou Wu Natalia Sevane Zhiqiang Li Chunhua Huang Jacqueline Smith Emiliano Lasagna Martino Cassandro Mauro Penasa Genomics of Dwarfism in Italian Local Chicken Breeds Genes genomic region indigenous breed chicken SNP GWAS |
title | Genomics of Dwarfism in Italian Local Chicken Breeds |
title_full | Genomics of Dwarfism in Italian Local Chicken Breeds |
title_fullStr | Genomics of Dwarfism in Italian Local Chicken Breeds |
title_full_unstemmed | Genomics of Dwarfism in Italian Local Chicken Breeds |
title_short | Genomics of Dwarfism in Italian Local Chicken Breeds |
title_sort | genomics of dwarfism in italian local chicken breeds |
topic | genomic region indigenous breed chicken SNP GWAS |
url | https://www.mdpi.com/2073-4425/14/3/633 |
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