Integrated Omics Reveal Time-Resolved Insights into T4 Phage Infection of <i>E. coli</i> on Proteome and Transcriptome Levels
Bacteriophages are highly abundant viruses of bacteria. The major role of phages in shaping bacterial communities and their emerging medical potential as antibacterial agents has triggered a rebirth of phage research. To understand the molecular mechanisms by which phages hijack their host, omics te...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/11/2502 |
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| author | Maik Wolfram-Schauerte Nadiia Pozhydaieva Madita Viering Timo Glatter Katharina Höfer |
| author_facet | Maik Wolfram-Schauerte Nadiia Pozhydaieva Madita Viering Timo Glatter Katharina Höfer |
| author_sort | Maik Wolfram-Schauerte |
| collection | DOAJ |
| description | Bacteriophages are highly abundant viruses of bacteria. The major role of phages in shaping bacterial communities and their emerging medical potential as antibacterial agents has triggered a rebirth of phage research. To understand the molecular mechanisms by which phages hijack their host, omics technologies can provide novel insights into the organization of transcriptional and translational events occurring during the infection process. In this study, we apply transcriptomics and proteomics to characterize the temporal patterns of transcription and protein synthesis during the T4 phage infection of <i>E. coli</i>. We investigated the stability of <i>E. coli</i>-originated transcripts and proteins in the course of infection, identifying the degradation of <i>E. coli</i> transcripts and the preservation of the host proteome. Moreover, the correlation between the phage transcriptome and proteome reveals specific T4 phage mRNAs and proteins that are temporally decoupled, suggesting post-transcriptional and translational regulation mechanisms. This study provides the first comprehensive insights into the molecular takeover of <i>E. coli</i> by bacteriophage T4. This data set represents a valuable resource for future studies seeking to study molecular and regulatory events during infection. We created a user-friendly online tool, POTATO4, which is available to the scientific community and allows access to gene expression patterns for <i>E. coli</i> and T4 genes. |
| first_indexed | 2024-03-09T17:56:24Z |
| format | Article |
| id | doaj.art-a15adeb8e7dd4759a465e6ccc4ecadb6 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T17:56:24Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a15adeb8e7dd4759a465e6ccc4ecadb62023-11-24T10:18:14ZengMDPI AGViruses1999-49152022-11-011411250210.3390/v14112502Integrated Omics Reveal Time-Resolved Insights into T4 Phage Infection of <i>E. coli</i> on Proteome and Transcriptome LevelsMaik Wolfram-Schauerte0Nadiia Pozhydaieva1Madita Viering2Timo Glatter3Katharina Höfer4Max-Planck-Institute for Terrestrial Microbiology, 35043 Marburg, GermanyMax-Planck-Institute for Terrestrial Microbiology, 35043 Marburg, GermanyMax-Planck-Institute for Terrestrial Microbiology, 35043 Marburg, GermanyMax-Planck-Institute for Terrestrial Microbiology, 35043 Marburg, GermanyMax-Planck-Institute for Terrestrial Microbiology, 35043 Marburg, GermanyBacteriophages are highly abundant viruses of bacteria. The major role of phages in shaping bacterial communities and their emerging medical potential as antibacterial agents has triggered a rebirth of phage research. To understand the molecular mechanisms by which phages hijack their host, omics technologies can provide novel insights into the organization of transcriptional and translational events occurring during the infection process. In this study, we apply transcriptomics and proteomics to characterize the temporal patterns of transcription and protein synthesis during the T4 phage infection of <i>E. coli</i>. We investigated the stability of <i>E. coli</i>-originated transcripts and proteins in the course of infection, identifying the degradation of <i>E. coli</i> transcripts and the preservation of the host proteome. Moreover, the correlation between the phage transcriptome and proteome reveals specific T4 phage mRNAs and proteins that are temporally decoupled, suggesting post-transcriptional and translational regulation mechanisms. This study provides the first comprehensive insights into the molecular takeover of <i>E. coli</i> by bacteriophage T4. This data set represents a valuable resource for future studies seeking to study molecular and regulatory events during infection. We created a user-friendly online tool, POTATO4, which is available to the scientific community and allows access to gene expression patterns for <i>E. coli</i> and T4 genes.https://www.mdpi.com/1999-4915/14/11/2502transcriptomicsproteomicsbacteriophage T4<i>Escherichia coli</i>phage infection |
| spellingShingle | Maik Wolfram-Schauerte Nadiia Pozhydaieva Madita Viering Timo Glatter Katharina Höfer Integrated Omics Reveal Time-Resolved Insights into T4 Phage Infection of <i>E. coli</i> on Proteome and Transcriptome Levels Viruses transcriptomics proteomics bacteriophage T4 <i>Escherichia coli</i> phage infection |
| title | Integrated Omics Reveal Time-Resolved Insights into T4 Phage Infection of <i>E. coli</i> on Proteome and Transcriptome Levels |
| title_full | Integrated Omics Reveal Time-Resolved Insights into T4 Phage Infection of <i>E. coli</i> on Proteome and Transcriptome Levels |
| title_fullStr | Integrated Omics Reveal Time-Resolved Insights into T4 Phage Infection of <i>E. coli</i> on Proteome and Transcriptome Levels |
| title_full_unstemmed | Integrated Omics Reveal Time-Resolved Insights into T4 Phage Infection of <i>E. coli</i> on Proteome and Transcriptome Levels |
| title_short | Integrated Omics Reveal Time-Resolved Insights into T4 Phage Infection of <i>E. coli</i> on Proteome and Transcriptome Levels |
| title_sort | integrated omics reveal time resolved insights into t4 phage infection of i e coli i on proteome and transcriptome levels |
| topic | transcriptomics proteomics bacteriophage T4 <i>Escherichia coli</i> phage infection |
| url | https://www.mdpi.com/1999-4915/14/11/2502 |
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