Box–Behnken Response Surface Design of Polysaccharide Extraction from <i>Rhododendron arboreum</i> and the Evaluation of Its Antioxidant Potential
In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box–Behnken response surface design and the evaluation of its antioxidant potential. Three parameters that affect the productivity...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/17/3835 |
| _version_ | 1797556108980649984 |
|---|---|
| author | Ajaz Ahmad Muneeb U. Rehman Adil Farooq Wali Hamed A. El-Serehy Fahad A. Al-Misned Saleh N. Maodaa Hossam M. Aljawdah Tahir Maqbool Mir Parvaiz Ahmad |
| author_facet | Ajaz Ahmad Muneeb U. Rehman Adil Farooq Wali Hamed A. El-Serehy Fahad A. Al-Misned Saleh N. Maodaa Hossam M. Aljawdah Tahir Maqbool Mir Parvaiz Ahmad |
| author_sort | Ajaz Ahmad |
| collection | DOAJ |
| description | In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box–Behnken response surface design and the evaluation of its antioxidant potential. Three parameters that affect the productivity of UAE, such as extraction temperature (50–90 °C), extraction time (10–30 min), and solid–liquid ratio (1–2 g/mL), were examined to optimize the yield of the polysaccharide percentage. The chromatographic analysis revealed that the composition of monosaccharides was found to be glucose, galactose, mannose, arabinose, and fucose. The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R<sup>2</sup> = 0.999). The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage. Derringer’s desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid–solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments. An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity. The in vitro antioxidant effect of polysaccharides of R. arboreum was studied. The results showed that the RAP extract exhibited a strong potential against free radical damage. |
| first_indexed | 2024-03-10T16:57:06Z |
| format | Article |
| id | doaj.art-a15f68c9502f48939ad53a06c00a77bc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:57:06Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a15f68c9502f48939ad53a06c00a77bc2023-11-20T11:06:38ZengMDPI AGMolecules1420-30492020-08-012517383510.3390/molecules25173835Box–Behnken Response Surface Design of Polysaccharide Extraction from <i>Rhododendron arboreum</i> and the Evaluation of Its Antioxidant PotentialAjaz Ahmad0Muneeb U. Rehman1Adil Farooq Wali2Hamed A. El-Serehy3Fahad A. Al-Misned4Saleh N. Maodaa5Hossam M. Aljawdah6Tahir Maqbool Mir7Parvaiz Ahmad8Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, RAK College of Pharmaceutical Sciences, RAK Medical and Health Science University, Ras Al Khaimah 11172, UAEDepartment of Zoology, College of Science, King Saud University, Riyadh l1451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh l1451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh l1451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh l1451, Saudi ArabiaNational Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, Oxford, MS 38677, USADepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaIn the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box–Behnken response surface design and the evaluation of its antioxidant potential. Three parameters that affect the productivity of UAE, such as extraction temperature (50–90 °C), extraction time (10–30 min), and solid–liquid ratio (1–2 g/mL), were examined to optimize the yield of the polysaccharide percentage. The chromatographic analysis revealed that the composition of monosaccharides was found to be glucose, galactose, mannose, arabinose, and fucose. The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R<sup>2</sup> = 0.999). The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage. Derringer’s desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid–solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments. An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity. The in vitro antioxidant effect of polysaccharides of R. arboreum was studied. The results showed that the RAP extract exhibited a strong potential against free radical damage.https://www.mdpi.com/1420-3049/25/17/3835<i>Rhododendron arboreum</i>polysaccharidesBox–Behnken designantioxidant activity |
| spellingShingle | Ajaz Ahmad Muneeb U. Rehman Adil Farooq Wali Hamed A. El-Serehy Fahad A. Al-Misned Saleh N. Maodaa Hossam M. Aljawdah Tahir Maqbool Mir Parvaiz Ahmad Box–Behnken Response Surface Design of Polysaccharide Extraction from <i>Rhododendron arboreum</i> and the Evaluation of Its Antioxidant Potential Molecules <i>Rhododendron arboreum</i> polysaccharides Box–Behnken design antioxidant activity |
| title | Box–Behnken Response Surface Design of Polysaccharide Extraction from <i>Rhododendron arboreum</i> and the Evaluation of Its Antioxidant Potential |
| title_full | Box–Behnken Response Surface Design of Polysaccharide Extraction from <i>Rhododendron arboreum</i> and the Evaluation of Its Antioxidant Potential |
| title_fullStr | Box–Behnken Response Surface Design of Polysaccharide Extraction from <i>Rhododendron arboreum</i> and the Evaluation of Its Antioxidant Potential |
| title_full_unstemmed | Box–Behnken Response Surface Design of Polysaccharide Extraction from <i>Rhododendron arboreum</i> and the Evaluation of Its Antioxidant Potential |
| title_short | Box–Behnken Response Surface Design of Polysaccharide Extraction from <i>Rhododendron arboreum</i> and the Evaluation of Its Antioxidant Potential |
| title_sort | box behnken response surface design of polysaccharide extraction from i rhododendron arboreum i and the evaluation of its antioxidant potential |
| topic | <i>Rhododendron arboreum</i> polysaccharides Box–Behnken design antioxidant activity |
| url | https://www.mdpi.com/1420-3049/25/17/3835 |
| work_keys_str_mv | AT ajazahmad boxbehnkenresponsesurfacedesignofpolysaccharideextractionfromirhododendronarboreumiandtheevaluationofitsantioxidantpotential AT muneeburehman boxbehnkenresponsesurfacedesignofpolysaccharideextractionfromirhododendronarboreumiandtheevaluationofitsantioxidantpotential AT adilfarooqwali boxbehnkenresponsesurfacedesignofpolysaccharideextractionfromirhododendronarboreumiandtheevaluationofitsantioxidantpotential AT hamedaelserehy boxbehnkenresponsesurfacedesignofpolysaccharideextractionfromirhododendronarboreumiandtheevaluationofitsantioxidantpotential AT fahadaalmisned boxbehnkenresponsesurfacedesignofpolysaccharideextractionfromirhododendronarboreumiandtheevaluationofitsantioxidantpotential AT salehnmaodaa boxbehnkenresponsesurfacedesignofpolysaccharideextractionfromirhododendronarboreumiandtheevaluationofitsantioxidantpotential AT hossammaljawdah boxbehnkenresponsesurfacedesignofpolysaccharideextractionfromirhododendronarboreumiandtheevaluationofitsantioxidantpotential AT tahirmaqboolmir boxbehnkenresponsesurfacedesignofpolysaccharideextractionfromirhododendronarboreumiandtheevaluationofitsantioxidantpotential AT parvaizahmad boxbehnkenresponsesurfacedesignofpolysaccharideextractionfromirhododendronarboreumiandtheevaluationofitsantioxidantpotential |