The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis
Serum anti-citrullinated protein antibodies (ACPAs), present in 70% of people with rheumatoid arthritis (RA), can be detected ≤10years before the onset of clinical disease. RA and periodontitis are epidemiologically associated and we have reported a high incidence of periodontitis in people who are...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-05-01
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| Series: | Journal of Oral Microbiology |
| Online Access: | http://dx.doi.org/10.1080/20002297.2017.1325216 |
| _version_ | 1818082755203825664 |
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| author | Z. Cheng T. Do K. Mankia J.L. Meade L. Hunt J. Nam A. Tugnait A. Speirs V. Clerehugh P. Emery D. Devine |
| author_facet | Z. Cheng T. Do K. Mankia J.L. Meade L. Hunt J. Nam A. Tugnait A. Speirs V. Clerehugh P. Emery D. Devine |
| author_sort | Z. Cheng |
| collection | DOAJ |
| description | Serum anti-citrullinated protein antibodies (ACPAs), present in 70% of people with rheumatoid arthritis (RA), can be detected ≤10years before the onset of clinical disease. RA and periodontitis are epidemiologically associated and we have reported a high incidence of periodontitis in people who are ACPA+ and at risk of RA. Periodontal bacteria may contribute by multiple routes to the generation of RA-autoantibodies. This study aims to characterise the subgingival microbiomes from periodontitis and health in individuals with/without RA and at risk of RA. Forty-five ACPA+ no RA (RA-at-risk; RAR), 31 healthy controls (HC) and 30 ACPA+ RA patients (RA) underwent a periodontal examination. DNA from subgingival plaque from healthy and deep pocket sites were paired-end sequenced using the Illumina HiSeq3000 and data analysed using MG-RAST + DESeq. Metagenomes in RA samples had high proportions of Actinobacteria; RAR microbiomes contained higher proportions of Bacteroidetes than HC. The relative abundance of P. gingivalis was high in periodontitis and RAR; Aggregatibacter actinomycetemcomitans was detected with similar frequency in each group. Other bacteria implicated in periodontitis and/or autoantibody generation (Filifactor alocis, Prevotella spp, Leptotrichia spp.) were detected. Analyses are on-going to elucidate the diversity and functional potential of the subgingival microbiome associated with RA. |
| first_indexed | 2024-12-10T19:27:08Z |
| format | Article |
| id | doaj.art-a15fa56cbaea467f92ba1998ef60160a |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-10T19:27:08Z |
| publishDate | 2017-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-a15fa56cbaea467f92ba1998ef60160a2022-12-22T01:36:21ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972017-05-019010.1080/20002297.2017.13252161325216The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritisZ. Cheng0T. Do1K. Mankia2J.L. Meade3L. Hunt4J. Nam5A. Tugnait6A. Speirs7V. Clerehugh8P. Emery9D. Devine10University of Leeds, School of DentistryUniversity of Leeds, School of DentistryUniversity of Leeds, School of MedicineUniversity of Leeds, School of DentistryUniversity of Leeds, School of MedicineUniversity of Leeds, School of MedicineUniversity of Leeds, School of DentistryLeeds Teaching Hospitals TrustUniversity of Leeds, School of DentistryUniversity of Leeds, School of MedicineUniversity of Leeds, School of DentistrySerum anti-citrullinated protein antibodies (ACPAs), present in 70% of people with rheumatoid arthritis (RA), can be detected ≤10years before the onset of clinical disease. RA and periodontitis are epidemiologically associated and we have reported a high incidence of periodontitis in people who are ACPA+ and at risk of RA. Periodontal bacteria may contribute by multiple routes to the generation of RA-autoantibodies. This study aims to characterise the subgingival microbiomes from periodontitis and health in individuals with/without RA and at risk of RA. Forty-five ACPA+ no RA (RA-at-risk; RAR), 31 healthy controls (HC) and 30 ACPA+ RA patients (RA) underwent a periodontal examination. DNA from subgingival plaque from healthy and deep pocket sites were paired-end sequenced using the Illumina HiSeq3000 and data analysed using MG-RAST + DESeq. Metagenomes in RA samples had high proportions of Actinobacteria; RAR microbiomes contained higher proportions of Bacteroidetes than HC. The relative abundance of P. gingivalis was high in periodontitis and RAR; Aggregatibacter actinomycetemcomitans was detected with similar frequency in each group. Other bacteria implicated in periodontitis and/or autoantibody generation (Filifactor alocis, Prevotella spp, Leptotrichia spp.) were detected. Analyses are on-going to elucidate the diversity and functional potential of the subgingival microbiome associated with RA.http://dx.doi.org/10.1080/20002297.2017.1325216 |
| spellingShingle | Z. Cheng T. Do K. Mankia J.L. Meade L. Hunt J. Nam A. Tugnait A. Speirs V. Clerehugh P. Emery D. Devine The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis Journal of Oral Microbiology |
| title | The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis |
| title_full | The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis |
| title_fullStr | The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis |
| title_full_unstemmed | The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis |
| title_short | The subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis |
| title_sort | subgingival microbiomes in periodontitis and health of individuals with rheumatoid arthritis and at risk of developing rheumatoid arthritis |
| url | http://dx.doi.org/10.1080/20002297.2017.1325216 |
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