Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts
Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C....
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MDPI AG
2017-09-01
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| Online Access: | https://www.mdpi.com/1420-3049/22/9/1489 |
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| author | Seung-Hui Song Sung Hwan Ki Dae-Hun Park Hong-Seop Moon Chang-Dai Lee In-Soo Yoon Seung-Sik Cho |
| author_facet | Seung-Hui Song Sung Hwan Ki Dae-Hun Park Hong-Seop Moon Chang-Dai Lee In-Soo Yoon Seung-Sik Cho |
| author_sort | Seung-Hui Song |
| collection | DOAJ |
| description | Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C. tricuspidata preparations has been reported. We developed and validated a reverse-phase high-performance liquid chromatography (HPLC) method for the separation and quantification of active markers. Ethanolic extracts of C. tricuspidata leaves were prepared and evaluated for chemical profiles and biological activities. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest total flavonoid content and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed that chlorogenic acid, rutin, and kaempferol were present in C. tricuspidata leaf extracts. We postulated that the antioxidant and anti-hyperuricemic/gout effects of C. tricuspidata extract could be attributed to these marker compounds. Our results suggested that the flavonoid-rich fraction of the leaf extract may be utilized for the treatment and prevention of hyperuricemia-related diseases, and the validated method and marker compounds could be applied for the quality control of C. tricuspidata preparations. |
| first_indexed | 2024-12-18T10:14:14Z |
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| id | doaj.art-a1604b9429264c91b546190ff5c349b3 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-18T10:14:14Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-a1604b9429264c91b546190ff5c349b32022-12-21T21:11:21ZengMDPI AGMolecules1420-30492017-09-01229148910.3390/molecules22091489molecules22091489Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit ExtractsSeung-Hui Song0Sung Hwan Ki1Dae-Hun Park2Hong-Seop Moon3Chang-Dai Lee4In-Soo Yoon5Seung-Sik Cho6Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, KoreaLaboratory of Toxicology, College of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, KoreaDepartment of Nursing, Dongshin University, Naju-si, Jeonnam 58245, KoreaDepartment of Pharmacy, College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, KoreaDepartment of Business Administration, Mokpo National University, Muan-gun, Jeonnam 58554, KoreaDepartment of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Geumjeong-gu, Busan 46241, KoreaDepartment of Pharmacy, College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, KoreaCudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C. tricuspidata preparations has been reported. We developed and validated a reverse-phase high-performance liquid chromatography (HPLC) method for the separation and quantification of active markers. Ethanolic extracts of C. tricuspidata leaves were prepared and evaluated for chemical profiles and biological activities. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest total flavonoid content and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed that chlorogenic acid, rutin, and kaempferol were present in C. tricuspidata leaf extracts. We postulated that the antioxidant and anti-hyperuricemic/gout effects of C. tricuspidata extract could be attributed to these marker compounds. Our results suggested that the flavonoid-rich fraction of the leaf extract may be utilized for the treatment and prevention of hyperuricemia-related diseases, and the validated method and marker compounds could be applied for the quality control of C. tricuspidata preparations.https://www.mdpi.com/1420-3049/22/9/1489C. tricuspidata Bureausimultaneous analysisHPLCxanthine oxidasehyperuricemia |
| spellingShingle | Seung-Hui Song Sung Hwan Ki Dae-Hun Park Hong-Seop Moon Chang-Dai Lee In-Soo Yoon Seung-Sik Cho Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts Molecules C. tricuspidata Bureau simultaneous analysis HPLC xanthine oxidase hyperuricemia |
| title | Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts |
| title_full | Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts |
| title_fullStr | Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts |
| title_full_unstemmed | Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts |
| title_short | Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts |
| title_sort | quantitative analysis extraction optimization and biological evaluation of cudrania tricuspidata leaf and fruit extracts |
| topic | C. tricuspidata Bureau simultaneous analysis HPLC xanthine oxidase hyperuricemia |
| url | https://www.mdpi.com/1420-3049/22/9/1489 |
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