Cytotoxicity Induced by Newly Synthesized Palladium (II) Complexes Lead to the Death of MCF-7 and MDA-MB-435 Cancer Cell Lines
Purpose: Breast cancer is the most common female malignancy and melanoma is the most lethal type of skin cancer. Traditional therapy for cancer treatment is far from satisfactory due to drug resistance and side effects, thus a search for new medicines is being emphasized. Palladium(II) complexes hav...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2023-01-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | https://apb.tbzmed.ac.ir/PDF/apb-13-160.pdf |
| _version_ | 1797673463192748032 |
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| author | Bruna Alexandre Oliveira da Silva Isabela Spido Dias Luís Eduardo Sarto Elba Pereira de Gois Claudia Torres Eduardo Tonon de Almeida Cibele Marli Cação Paiva Gouvêa |
| author_facet | Bruna Alexandre Oliveira da Silva Isabela Spido Dias Luís Eduardo Sarto Elba Pereira de Gois Claudia Torres Eduardo Tonon de Almeida Cibele Marli Cação Paiva Gouvêa |
| author_sort | Bruna Alexandre Oliveira da Silva |
| collection | DOAJ |
| description | Purpose: Breast cancer is the most common female malignancy and melanoma is the most lethal type of skin cancer. Traditional therapy for cancer treatment is far from satisfactory due to drug resistance and side effects, thus a search for new medicines is being emphasized. Palladium(II) complexes have been reported as anticancer potential agents. In this work, the anticancer activities and cell death induction of a new series of square-planar Pd(II) complexes were evaluated against MCF-7 and MDA-MB-435 cancer cells. Methods: MCF-7 (breast carcinoma) and MDA-MB-435 (melanoma) cells were cultivated, and treated with ligand and Pd(II) complexes. Cell growth, migration and adhesion inhibition, morphological alterations, cell death induction and, DNA interaction upon treatment were studied. Results: Pd(II) complexes exhibited both short and long-term antiproliferative effects on both cell lines, reducing by 80% cell growth in the SRB assay and abolishing longterm proliferation, estimated by the clonogenic assay. Complexes reduced significantly (P<0.05) cell migration and adhesion when compared to the control group. Complexes induced morphological alterations in cell lines and significant (P<0.05) cellular shrinkage. Cell death was induced and the complexes were able to interact with DNA, inducing cleavage of double-stranded DNA, which may account for the complexes cytotoxic effects, observed against both MCF-7 and MDA-MB-435 cells. Conclusion: Overall, the complexes exhibited cytotoxic activities and induced cell death. These observations emphasize an anticancer role with a potential therapeutic value for Pd(II) complexes to improve the outcome of patients with breast cancer and melanoma. |
| first_indexed | 2024-03-11T21:44:47Z |
| format | Article |
| id | doaj.art-a1646ff7b76a4200b91050e12f5d5df5 |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-03-11T21:44:47Z |
| publishDate | 2023-01-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-a1646ff7b76a4200b91050e12f5d5df52023-09-26T10:13:31ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082023-01-0113116016910.34172/apb.2023.017apb-32523Cytotoxicity Induced by Newly Synthesized Palladium (II) Complexes Lead to the Death of MCF-7 and MDA-MB-435 Cancer Cell LinesBruna Alexandre Oliveira da Silva0Isabela Spido Dias1Luís Eduardo Sarto2Elba Pereira de Gois3Claudia Torres4Eduardo Tonon de Almeida5Cibele Marli Cação Paiva Gouvêa6Institute of Natural Sciences, Federal University of Alfenas, Alfenas, Brazil.Institute of Natural Sciences, Federal University of Alfenas, Alfenas, Brazil.Federal Institute of Education, Science and Technology of the South of Minas Gerais, Machado, Brazil.Institute of Chemistry, Federal University of Alfenas, Alfenas, Brazil.Institute of Chemistry, Federal University of Alfenas, Alfenas, Brazil.Institute of Chemistry, Federal University of Alfenas, Alfenas, Brazil.Institute of Natural Sciences, Federal University of Alfenas, Alfenas, Brazil.Purpose: Breast cancer is the most common female malignancy and melanoma is the most lethal type of skin cancer. Traditional therapy for cancer treatment is far from satisfactory due to drug resistance and side effects, thus a search for new medicines is being emphasized. Palladium(II) complexes have been reported as anticancer potential agents. In this work, the anticancer activities and cell death induction of a new series of square-planar Pd(II) complexes were evaluated against MCF-7 and MDA-MB-435 cancer cells. Methods: MCF-7 (breast carcinoma) and MDA-MB-435 (melanoma) cells were cultivated, and treated with ligand and Pd(II) complexes. Cell growth, migration and adhesion inhibition, morphological alterations, cell death induction and, DNA interaction upon treatment were studied. Results: Pd(II) complexes exhibited both short and long-term antiproliferative effects on both cell lines, reducing by 80% cell growth in the SRB assay and abolishing longterm proliferation, estimated by the clonogenic assay. Complexes reduced significantly (P<0.05) cell migration and adhesion when compared to the control group. Complexes induced morphological alterations in cell lines and significant (P<0.05) cellular shrinkage. Cell death was induced and the complexes were able to interact with DNA, inducing cleavage of double-stranded DNA, which may account for the complexes cytotoxic effects, observed against both MCF-7 and MDA-MB-435 cells. Conclusion: Overall, the complexes exhibited cytotoxic activities and induced cell death. These observations emphasize an anticancer role with a potential therapeutic value for Pd(II) complexes to improve the outcome of patients with breast cancer and melanoma.https://apb.tbzmed.ac.ir/PDF/apb-13-160.pdfanticancer agentbreast cancercell migrationcell viabilitymelanomametal complexes |
| spellingShingle | Bruna Alexandre Oliveira da Silva Isabela Spido Dias Luís Eduardo Sarto Elba Pereira de Gois Claudia Torres Eduardo Tonon de Almeida Cibele Marli Cação Paiva Gouvêa Cytotoxicity Induced by Newly Synthesized Palladium (II) Complexes Lead to the Death of MCF-7 and MDA-MB-435 Cancer Cell Lines Advanced Pharmaceutical Bulletin anticancer agent breast cancer cell migration cell viability melanoma metal complexes |
| title | Cytotoxicity Induced by Newly Synthesized Palladium (II) Complexes Lead to the Death of MCF-7 and MDA-MB-435 Cancer Cell Lines |
| title_full | Cytotoxicity Induced by Newly Synthesized Palladium (II) Complexes Lead to the Death of MCF-7 and MDA-MB-435 Cancer Cell Lines |
| title_fullStr | Cytotoxicity Induced by Newly Synthesized Palladium (II) Complexes Lead to the Death of MCF-7 and MDA-MB-435 Cancer Cell Lines |
| title_full_unstemmed | Cytotoxicity Induced by Newly Synthesized Palladium (II) Complexes Lead to the Death of MCF-7 and MDA-MB-435 Cancer Cell Lines |
| title_short | Cytotoxicity Induced by Newly Synthesized Palladium (II) Complexes Lead to the Death of MCF-7 and MDA-MB-435 Cancer Cell Lines |
| title_sort | cytotoxicity induced by newly synthesized palladium ii complexes lead to the death of mcf 7 and mda mb 435 cancer cell lines |
| topic | anticancer agent breast cancer cell migration cell viability melanoma metal complexes |
| url | https://apb.tbzmed.ac.ir/PDF/apb-13-160.pdf |
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