New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species
Hypochlorous acid (HOCl) derived from hydrogen peroxide and chloride anion by myeloperoxidase (MPO) plays a significant role in physiological and pathological processes. Herein we report a phenoxazine-based fluorescent probe Celestine Blue B (CB) that is applicable for HOCl detection in living cells...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/9/1719 |
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| author | Veronika E. Reut Stanislav O. Kozlov Igor V. Kudryavtsev Natalya A. Grudinina Valeria A. Kostevich Nikolay P. Gorbunov Daria V. Grigorieva Julia A. Kalvinkovskaya Sergey B. Bushuk Elena Yu Varfolomeeva Natalia D. Fedorova Irina V. Gorudko Oleg M. Panasenko Vadim B. Vasilyev Alexey V. Sokolov |
| author_facet | Veronika E. Reut Stanislav O. Kozlov Igor V. Kudryavtsev Natalya A. Grudinina Valeria A. Kostevich Nikolay P. Gorbunov Daria V. Grigorieva Julia A. Kalvinkovskaya Sergey B. Bushuk Elena Yu Varfolomeeva Natalia D. Fedorova Irina V. Gorudko Oleg M. Panasenko Vadim B. Vasilyev Alexey V. Sokolov |
| author_sort | Veronika E. Reut |
| collection | DOAJ |
| description | Hypochlorous acid (HOCl) derived from hydrogen peroxide and chloride anion by myeloperoxidase (MPO) plays a significant role in physiological and pathological processes. Herein we report a phenoxazine-based fluorescent probe Celestine Blue B (CB) that is applicable for HOCl detection in living cells and for assaying the chlorinating activity of MPO. A remarkable selectivity and sensitivity (limit of detection is 32 nM), along with a rapid “turn-on” response of CB to HOCl was demonstrated. Furthermore, the probe was able to detect endogenous HOCl and reactive halogenated species by fluorescence spectroscopy, confocal microscopy, and flow cytometry techniques. Hence, CB is a promising tool for investigating the role of HOCl in health and disease and for screening the drugs capable of regulating MPO activity. |
| first_indexed | 2024-03-10T00:53:08Z |
| format | Article |
| id | doaj.art-a1654d7ddb564803bef79c003a3c7cba |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T00:53:08Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a1654d7ddb564803bef79c003a3c7cba2023-11-23T14:47:49ZengMDPI AGAntioxidants2076-39212022-08-01119171910.3390/antiox11091719New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated SpeciesVeronika E. Reut0Stanislav O. Kozlov1Igor V. Kudryavtsev2Natalya A. Grudinina3Valeria A. Kostevich4Nikolay P. Gorbunov5Daria V. Grigorieva6Julia A. Kalvinkovskaya7Sergey B. Bushuk8Elena Yu Varfolomeeva9Natalia D. Fedorova10Irina V. Gorudko11Oleg M. Panasenko12Vadim B. Vasilyev13Alexey V. Sokolov14Department of Biophysics, Belarusian State University, 220030 Minsk, BelarusDepartment of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Immunology, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Biophysics, Belarusian State University, 220030 Minsk, BelarusStepanov Institute of Physics, National Academy of Sciences of Belarus, 220072 Minsk, BelarusSSPA “Optics, Optoelectronics, and Laser Technology”, 220072 Minsk, BelarusPetersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaDepartment of Biophysics, Belarusian State University, 220030 Minsk, BelarusDepartment of Biophysics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaDepartment of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaHypochlorous acid (HOCl) derived from hydrogen peroxide and chloride anion by myeloperoxidase (MPO) plays a significant role in physiological and pathological processes. Herein we report a phenoxazine-based fluorescent probe Celestine Blue B (CB) that is applicable for HOCl detection in living cells and for assaying the chlorinating activity of MPO. A remarkable selectivity and sensitivity (limit of detection is 32 nM), along with a rapid “turn-on” response of CB to HOCl was demonstrated. Furthermore, the probe was able to detect endogenous HOCl and reactive halogenated species by fluorescence spectroscopy, confocal microscopy, and flow cytometry techniques. Hence, CB is a promising tool for investigating the role of HOCl in health and disease and for screening the drugs capable of regulating MPO activity.https://www.mdpi.com/2076-3921/11/9/1719hypochlorous acidmyeloperoxidaseneutrophilsfluorescent probesreactive halogen speciesphenoxazine |
| spellingShingle | Veronika E. Reut Stanislav O. Kozlov Igor V. Kudryavtsev Natalya A. Grudinina Valeria A. Kostevich Nikolay P. Gorbunov Daria V. Grigorieva Julia A. Kalvinkovskaya Sergey B. Bushuk Elena Yu Varfolomeeva Natalia D. Fedorova Irina V. Gorudko Oleg M. Panasenko Vadim B. Vasilyev Alexey V. Sokolov New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species Antioxidants hypochlorous acid myeloperoxidase neutrophils fluorescent probes reactive halogen species phenoxazine |
| title | New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species |
| title_full | New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species |
| title_fullStr | New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species |
| title_full_unstemmed | New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species |
| title_short | New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species |
| title_sort | new application of the commercially available dye celestine blue b as a sensitive and selective fluorescent turn on probe for endogenous detection of hocl and reactive halogenated species |
| topic | hypochlorous acid myeloperoxidase neutrophils fluorescent probes reactive halogen species phenoxazine |
| url | https://www.mdpi.com/2076-3921/11/9/1719 |
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