Adventitial histopathological changes after coronary stenting in a porcine model
The adverse long-term events in first-generation drug-eluting stents were associated with chronic inflammatory response to the polymer. As an alternative, stents with biodegradable polymers emerged, whose effects on the vascular response are not yet fully known. Our objectives were to study the adve...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2020-11-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202011-0001_adventitial-histopathological-changes-after-coronary-stenting-in-8239-a-porcine-model.php |
| _version_ | 1797897455950364672 |
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| author | Claudia Perez-Martinez Armando Perez de Prado Vanesa Caballero-Manso Marta Regueiro-Purrinos Gracia Mª de Garnica Garcia Carlos Cuellas-Ramon Jose Manuel Gonzalo-Orden Maria Lopez-Benito Jose R. Altonaga Tomas Benito-Gomez Felipe Fernandez-Vazquez |
| author_facet | Claudia Perez-Martinez Armando Perez de Prado Vanesa Caballero-Manso Marta Regueiro-Purrinos Gracia Mª de Garnica Garcia Carlos Cuellas-Ramon Jose Manuel Gonzalo-Orden Maria Lopez-Benito Jose R. Altonaga Tomas Benito-Gomez Felipe Fernandez-Vazquez |
| author_sort | Claudia Perez-Martinez |
| collection | DOAJ |
| description | The adverse long-term events in first-generation drug-eluting stents were associated with chronic inflammatory response to the polymer. As an alternative, stents with biodegradable polymers emerged, whose effects on the vascular response are not yet fully known. Our objectives were to study the adventitial response to the stent implantation and the role of the polymeric vehicle. A histological (Haematoxylin-Eosin, Verhoeff van Gieson) and immunohistochemical (von Willebrand factor, alpha-smooth muscle actin) analysis were performed on resin-embedded arterial sections from fifteen Large White pigs, 28 days after the random implantation in the coronary arteries of: a chromium-cobalt stent and a stent coated with a permanent polyacrylate or biodegradable poly(D,L)-lactic-co-glycolic polymer, the two latter ones are loaded with sirolimus. Independent of the stent, the adventitial inflammation was associated with the adventitial area (P = 0.006 8) and the inflammation score (P = 0.037 1); and the adventitial actin-positive cells with the vascular damage (P = 0.001 2). A significant relationship was observed between the greater percentages of the restenosis and the more intense inflammation (P = 0.035 1) and the actin-positive cells (P = 0.011 9) in the adventitia. The polymeric vehicle increased the adventitial actin-positive cells (P = 0.018), independent of the type of polymer. The adventitial changes seem to be related to the restenosic process 28 days after the coronary stenting. Further investigations are necessary to confirm the role of the polymeric vehicle on the adventitial histopathological changes. |
| first_indexed | 2024-04-10T07:58:48Z |
| format | Article |
| id | doaj.art-a16afee4a6b940b993e5bd2f87303766 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:58:48Z |
| publishDate | 2020-11-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-a16afee4a6b940b993e5bd2f873037662023-02-23T03:50:59ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922020-11-01651146547210.17221/159/2019-VETMEDvet-202011-0001Adventitial histopathological changes after coronary stenting in a porcine modelClaudia Perez-Martinez0Armando Perez de Prado1Vanesa Caballero-Manso2Marta Regueiro-Purrinos3Gracia Mª de Garnica Garcia4Carlos Cuellas-Ramon5Jose Manuel Gonzalo-Orden6Maria Lopez-Benito7Jose R. Altonaga8Tomas Benito-Gomez9Felipe Fernandez-Vazquez10Department of Animal Health, Section of Pathology, Veterinary School, University of León, León, SpainInstitute of Biomedicine (IBIOMED), University of León, León, SpainDepartment of Animal Health, Section of Pathology, Veterinary School, University of León, León, SpainInstitute of Biomedicine (IBIOMED), University of León, León, SpainMicrosVeterinaria SL, León, SpainInstitute of Biomedicine (IBIOMED), University of León, León, SpainInstitute of Biomedicine (IBIOMED), University of León, León, SpainInstitute of Biomedicine (IBIOMED), University of León, León, SpainInstitute of Biomedicine (IBIOMED), University of León, León, SpainInstitute of Biomedicine (IBIOMED), University of León, León, SpainInstitute of Biomedicine (IBIOMED), University of León, León, SpainThe adverse long-term events in first-generation drug-eluting stents were associated with chronic inflammatory response to the polymer. As an alternative, stents with biodegradable polymers emerged, whose effects on the vascular response are not yet fully known. Our objectives were to study the adventitial response to the stent implantation and the role of the polymeric vehicle. A histological (Haematoxylin-Eosin, Verhoeff van Gieson) and immunohistochemical (von Willebrand factor, alpha-smooth muscle actin) analysis were performed on resin-embedded arterial sections from fifteen Large White pigs, 28 days after the random implantation in the coronary arteries of: a chromium-cobalt stent and a stent coated with a permanent polyacrylate or biodegradable poly(D,L)-lactic-co-glycolic polymer, the two latter ones are loaded with sirolimus. Independent of the stent, the adventitial inflammation was associated with the adventitial area (P = 0.006 8) and the inflammation score (P = 0.037 1); and the adventitial actin-positive cells with the vascular damage (P = 0.001 2). A significant relationship was observed between the greater percentages of the restenosis and the more intense inflammation (P = 0.035 1) and the actin-positive cells (P = 0.011 9) in the adventitia. The polymeric vehicle increased the adventitial actin-positive cells (P = 0.018), independent of the type of polymer. The adventitial changes seem to be related to the restenosic process 28 days after the coronary stenting. Further investigations are necessary to confirm the role of the polymeric vehicle on the adventitial histopathological changes.https://vetmed.agriculturejournals.cz/artkey/vet-202011-0001_adventitial-histopathological-changes-after-coronary-stenting-in-8239-a-porcine-model.phpadventitiapigpolymerrestenosisstent |
| spellingShingle | Claudia Perez-Martinez Armando Perez de Prado Vanesa Caballero-Manso Marta Regueiro-Purrinos Gracia Mª de Garnica Garcia Carlos Cuellas-Ramon Jose Manuel Gonzalo-Orden Maria Lopez-Benito Jose R. Altonaga Tomas Benito-Gomez Felipe Fernandez-Vazquez Adventitial histopathological changes after coronary stenting in a porcine model Veterinární Medicína adventitia pig polymer restenosis stent |
| title | Adventitial histopathological changes after coronary stenting in a porcine model |
| title_full | Adventitial histopathological changes after coronary stenting in a porcine model |
| title_fullStr | Adventitial histopathological changes after coronary stenting in a porcine model |
| title_full_unstemmed | Adventitial histopathological changes after coronary stenting in a porcine model |
| title_short | Adventitial histopathological changes after coronary stenting in a porcine model |
| title_sort | adventitial histopathological changes after coronary stenting in a porcine model |
| topic | adventitia pig polymer restenosis stent |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202011-0001_adventitial-histopathological-changes-after-coronary-stenting-in-8239-a-porcine-model.php |
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