Reduction of Human Glioblastoma Spheroids Using Cold Atmospheric Plasma: The Combined Effect of Short- and Long-Lived Reactive Species
Cold atmospheric plasma (CAP) is a promising technology against multiple types of cancer. However, the current findings on the effect of CAP on two-dimensional glioblastoma cultures do not consider the role of the tumour microenvironment. The aim of this study was to determine the ability of CAP to...
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| Format: | Article |
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MDPI AG
2018-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/10/11/394 |
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| author | Angela Privat-Maldonado Yury Gorbanev Sylvia Dewilde Evelien Smits Annemie Bogaerts |
| author_facet | Angela Privat-Maldonado Yury Gorbanev Sylvia Dewilde Evelien Smits Annemie Bogaerts |
| author_sort | Angela Privat-Maldonado |
| collection | DOAJ |
| description | Cold atmospheric plasma (CAP) is a promising technology against multiple types of cancer. However, the current findings on the effect of CAP on two-dimensional glioblastoma cultures do not consider the role of the tumour microenvironment. The aim of this study was to determine the ability of CAP to reduce and control glioblastoma spheroid tumours in vitro. Three-dimensional glioblastoma spheroid tumours (U87-Red, U251-Red) were consecutively treated directly and indirectly with a CAP using dry He, He + 5% H<sub>2</sub>O or He + 20% H<sub>2</sub>O. The cytotoxicity and spheroid shrinkage were monitored using live imaging. The reactive oxygen and nitrogen species produced in phosphate buffered saline (PBS) were measured by electron paramagnetic resonance (EPR) and colourimetry. Cell migration was also assessed. Our results demonstrate that consecutive CAP treatments (He + 20% H<sub>2</sub>O) substantially shrank U87-Red spheroids and to a lesser degree, U251-Red spheroids. The cytotoxic effect was due to the short- and long-lived species delivered by CAP: they inhibited spheroid growth, reduced cell migration and decreased proliferation in CAP-treated spheroids. Direct treatments were more effective than indirect treatments, suggesting the importance of CAP-generated, short-lived species for the growth inhibition and cell cytotoxicity of solid glioblastoma tumours. We concluded that CAP treatment can effectively reduce glioblastoma tumour size and restrict cell migration, thus demonstrating the potential of CAP therapies for glioblastoma. |
| first_indexed | 2024-03-12T19:33:41Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T19:33:41Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-a16b9aea2d41481ebd2deeaba7e7dc622023-08-02T04:21:43ZengMDPI AGCancers2072-66942018-10-01101139410.3390/cancers10110394cancers10110394Reduction of Human Glioblastoma Spheroids Using Cold Atmospheric Plasma: The Combined Effect of Short- and Long-Lived Reactive SpeciesAngela Privat-Maldonado0Yury Gorbanev1Sylvia Dewilde2Evelien Smits3Annemie Bogaerts4PLASMANT, Chemistry Department, University of Antwerp, 2610 Antwerp, BelgiumPLASMANT, Chemistry Department, University of Antwerp, 2610 Antwerp, BelgiumProtein Chemistry, Proteomics and Epigenetic Signaling, Department of Biomedical Sciences, University of Antwerp, 2610 Antwerp, BelgiumSolid Tumor Immunology Group, Center for Oncological Research, University of Antwerp, 2610 Antwerp, BelgiumPLASMANT, Chemistry Department, University of Antwerp, 2610 Antwerp, BelgiumCold atmospheric plasma (CAP) is a promising technology against multiple types of cancer. However, the current findings on the effect of CAP on two-dimensional glioblastoma cultures do not consider the role of the tumour microenvironment. The aim of this study was to determine the ability of CAP to reduce and control glioblastoma spheroid tumours in vitro. Three-dimensional glioblastoma spheroid tumours (U87-Red, U251-Red) were consecutively treated directly and indirectly with a CAP using dry He, He + 5% H<sub>2</sub>O or He + 20% H<sub>2</sub>O. The cytotoxicity and spheroid shrinkage were monitored using live imaging. The reactive oxygen and nitrogen species produced in phosphate buffered saline (PBS) were measured by electron paramagnetic resonance (EPR) and colourimetry. Cell migration was also assessed. Our results demonstrate that consecutive CAP treatments (He + 20% H<sub>2</sub>O) substantially shrank U87-Red spheroids and to a lesser degree, U251-Red spheroids. The cytotoxic effect was due to the short- and long-lived species delivered by CAP: they inhibited spheroid growth, reduced cell migration and decreased proliferation in CAP-treated spheroids. Direct treatments were more effective than indirect treatments, suggesting the importance of CAP-generated, short-lived species for the growth inhibition and cell cytotoxicity of solid glioblastoma tumours. We concluded that CAP treatment can effectively reduce glioblastoma tumour size and restrict cell migration, thus demonstrating the potential of CAP therapies for glioblastoma.https://www.mdpi.com/2072-6694/10/11/394cancercold atmospheric plasma (CAP)spheroid shrinkagecytotoxicitytumour reductionglioblastomashort-lived reactive speciescell migrationproliferation |
| spellingShingle | Angela Privat-Maldonado Yury Gorbanev Sylvia Dewilde Evelien Smits Annemie Bogaerts Reduction of Human Glioblastoma Spheroids Using Cold Atmospheric Plasma: The Combined Effect of Short- and Long-Lived Reactive Species Cancers cancer cold atmospheric plasma (CAP) spheroid shrinkage cytotoxicity tumour reduction glioblastoma short-lived reactive species cell migration proliferation |
| title | Reduction of Human Glioblastoma Spheroids Using Cold Atmospheric Plasma: The Combined Effect of Short- and Long-Lived Reactive Species |
| title_full | Reduction of Human Glioblastoma Spheroids Using Cold Atmospheric Plasma: The Combined Effect of Short- and Long-Lived Reactive Species |
| title_fullStr | Reduction of Human Glioblastoma Spheroids Using Cold Atmospheric Plasma: The Combined Effect of Short- and Long-Lived Reactive Species |
| title_full_unstemmed | Reduction of Human Glioblastoma Spheroids Using Cold Atmospheric Plasma: The Combined Effect of Short- and Long-Lived Reactive Species |
| title_short | Reduction of Human Glioblastoma Spheroids Using Cold Atmospheric Plasma: The Combined Effect of Short- and Long-Lived Reactive Species |
| title_sort | reduction of human glioblastoma spheroids using cold atmospheric plasma the combined effect of short and long lived reactive species |
| topic | cancer cold atmospheric plasma (CAP) spheroid shrinkage cytotoxicity tumour reduction glioblastoma short-lived reactive species cell migration proliferation |
| url | https://www.mdpi.com/2072-6694/10/11/394 |
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