Kappa Casein Gene Polymorphism in Holstein Chinese Cattle
Kappa casein gene polymorphism has received a considerable attention because of its correlation with milk quality, composition and technological properties. The polymorphism of kappa casein gene (K-CN) was detected in Holstein Chinese cattle. A 218 bp sequence in exon IV of 319 Holstein Chinese catt...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2010-10-01
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| Series: | Pakistan Veterinary Journal |
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| Online Access: | http://www.pvj.com.pk/pdf-files/30_4/203-206.pdf |
| _version_ | 1818484376373035008 |
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| author | A. E. Hamza1,*, X. L. Wang and Z. P.Yang |
| author_facet | A. E. Hamza1,*, X. L. Wang and Z. P.Yang |
| author_sort | A. E. Hamza1,*, X. L. Wang and Z. P.Yang |
| collection | DOAJ |
| description | Kappa casein gene polymorphism has received a considerable attention because of its correlation with milk quality, composition and technological properties. The polymorphism of kappa casein gene (K-CN) was detected in Holstein Chinese cattle. A 218 bp sequence in exon IV of 319 Holstein Chinese cattle blood samples were amplified using polymerase chain reaction-single strand conformation (PCR-SSCP) technique. Sequence analysis revealed one single nucleotide polymorphism (SNP) T/C SNP in exon 1V at nucleotides (80), moreover; three genotypes TT, TC and CC were also identified with following frequencies: 0.40, 0.34 and 0.26%, respectively. The allele frequency for T and C found to be 0.6 and 0.4 %, respectively. Allele frequencies in the population fitted with Hardy–Weinberg equilibrium (P>0.05). Analysis of genetic polymorphism of k-casein at exon 1V exhibited medium polymorphism information content (PIC=0.36). |
| first_indexed | 2024-12-10T15:54:31Z |
| format | Article |
| id | doaj.art-a16c5dbaba894249a5c0711614edf658 |
| institution | Directory Open Access Journal |
| issn | 0253-8318 |
| language | English |
| last_indexed | 2024-12-10T15:54:31Z |
| publishDate | 2010-10-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-a16c5dbaba894249a5c0711614edf6582022-12-22T01:42:42ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182010-10-01304203206Kappa Casein Gene Polymorphism in Holstein Chinese CattleA. E. Hamza1,*, X. L. Wang and Z. P.YangKappa casein gene polymorphism has received a considerable attention because of its correlation with milk quality, composition and technological properties. The polymorphism of kappa casein gene (K-CN) was detected in Holstein Chinese cattle. A 218 bp sequence in exon IV of 319 Holstein Chinese cattle blood samples were amplified using polymerase chain reaction-single strand conformation (PCR-SSCP) technique. Sequence analysis revealed one single nucleotide polymorphism (SNP) T/C SNP in exon 1V at nucleotides (80), moreover; three genotypes TT, TC and CC were also identified with following frequencies: 0.40, 0.34 and 0.26%, respectively. The allele frequency for T and C found to be 0.6 and 0.4 %, respectively. Allele frequencies in the population fitted with Hardy–Weinberg equilibrium (P>0.05). Analysis of genetic polymorphism of k-casein at exon 1V exhibited medium polymorphism information content (PIC=0.36).http://www.pvj.com.pk/pdf-files/30_4/203-206.pdfHolstein Chinese cattleKappa casein genePolymorphismPCR-SSCPSingle nucleotide |
| spellingShingle | A. E. Hamza1,*, X. L. Wang and Z. P.Yang Kappa Casein Gene Polymorphism in Holstein Chinese Cattle Pakistan Veterinary Journal Holstein Chinese cattle Kappa casein gene Polymorphism PCR-SSCP Single nucleotide |
| title | Kappa Casein Gene Polymorphism in Holstein Chinese Cattle |
| title_full | Kappa Casein Gene Polymorphism in Holstein Chinese Cattle |
| title_fullStr | Kappa Casein Gene Polymorphism in Holstein Chinese Cattle |
| title_full_unstemmed | Kappa Casein Gene Polymorphism in Holstein Chinese Cattle |
| title_short | Kappa Casein Gene Polymorphism in Holstein Chinese Cattle |
| title_sort | kappa casein gene polymorphism in holstein chinese cattle |
| topic | Holstein Chinese cattle Kappa casein gene Polymorphism PCR-SSCP Single nucleotide |
| url | http://www.pvj.com.pk/pdf-files/30_4/203-206.pdf |
| work_keys_str_mv | AT aehamza1xlwangandzpyang kappacaseingenepolymorphisminholsteinchinesecattle |