Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupin
Cell-wall resynthesis was studied in protoplast culture of yellow lupin (Lupinus luteus L.). We optimized protoplast isolation and found that explants excised from young seedling were more suitable sources of protoplasts, in contrast to callus tissue. Incubation in 2% cellulase R-10, 1% pectinase an...
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| Format: | Article |
| Language: | Bulgarian |
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University of Zagreb, Faculty of Agriculture
2013-03-01
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| Series: | Journal of Central European Agriculture |
| Subjects: | |
| Online Access: | http://jcea.agr.hr/articles/774634_Explant_dependent_receptivity_to_isolation_and_a_cell_wall_resynthesis_in_protoplast_culture_of_recalcitrant_yellow_lupin_en.pdf |
| _version_ | 1818825850390315008 |
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| author | Alina WISZNIEWSKA Anna PINDEL |
| author_facet | Alina WISZNIEWSKA Anna PINDEL |
| author_sort | Alina WISZNIEWSKA |
| collection | DOAJ |
| description | Cell-wall resynthesis was studied in protoplast culture of yellow lupin (Lupinus luteus L.). We optimized protoplast isolation and found that explants excised from young seedling were more suitable sources of protoplasts, in contrast to callus tissue. Incubation in 2% cellulase R-10, 1% pectinase and 0.5% macerozyme solution for 3h effectively released protoplasts from majority of tested explants. Furthermore, we determined the optimal developmental age of explants which was 4, 21, 25 and 35 days for hypocotyls, cotyledons, in-vitro leaf mesophyll and ex-vitro leaf mesophyll, respectively. Explant type, culture medium and genotype influenced both a rate and a pattern of the cell wall regeneration. After 10 days of culture, the number of regenerated cells reached 44%-59% in hypocotyl, 84%-91% in cotyledonary, and 31%-42% in mesophyll protoplasts. Our results show that the earlier wall regeneration begins, the wall surface will be more incomplete. We suggest that unbalanced and inefficient cell-wall resynthesis likely contributes to recalcitrance of yellow lupin to manipulations in protoplast technology. |
| first_indexed | 2024-12-19T00:18:19Z |
| format | Article |
| id | doaj.art-a16d8fb4cadb4691914fcae0a23edbcf |
| institution | Directory Open Access Journal |
| issn | 1332-9049 |
| language | Bulgarian |
| last_indexed | 2024-12-19T00:18:19Z |
| publishDate | 2013-03-01 |
| publisher | University of Zagreb, Faculty of Agriculture |
| record_format | Article |
| series | Journal of Central European Agriculture |
| spelling | doaj.art-a16d8fb4cadb4691914fcae0a23edbcf2022-12-21T20:45:41ZbulUniversity of Zagreb, Faculty of AgricultureJournal of Central European Agriculture1332-90492013-03-0114114315610.5513/JCEA01/14.1.1173Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupinAlina WISZNIEWSKAAnna PINDELCell-wall resynthesis was studied in protoplast culture of yellow lupin (Lupinus luteus L.). We optimized protoplast isolation and found that explants excised from young seedling were more suitable sources of protoplasts, in contrast to callus tissue. Incubation in 2% cellulase R-10, 1% pectinase and 0.5% macerozyme solution for 3h effectively released protoplasts from majority of tested explants. Furthermore, we determined the optimal developmental age of explants which was 4, 21, 25 and 35 days for hypocotyls, cotyledons, in-vitro leaf mesophyll and ex-vitro leaf mesophyll, respectively. Explant type, culture medium and genotype influenced both a rate and a pattern of the cell wall regeneration. After 10 days of culture, the number of regenerated cells reached 44%-59% in hypocotyl, 84%-91% in cotyledonary, and 31%-42% in mesophyll protoplasts. Our results show that the earlier wall regeneration begins, the wall surface will be more incomplete. We suggest that unbalanced and inefficient cell-wall resynthesis likely contributes to recalcitrance of yellow lupin to manipulations in protoplast technology.http://jcea.agr.hr/articles/774634_Explant_dependent_receptivity_to_isolation_and_a_cell_wall_resynthesis_in_protoplast_culture_of_recalcitrant_yellow_lupin_en.pdfcell wallfabaceaelupinus luteus l.protoplast cultureviability |
| spellingShingle | Alina WISZNIEWSKA Anna PINDEL Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupin Journal of Central European Agriculture cell wall fabaceae lupinus luteus l. protoplast culture viability |
| title | Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupin |
| title_full | Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupin |
| title_fullStr | Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupin |
| title_full_unstemmed | Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupin |
| title_short | Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupin |
| title_sort | explant dependent receptivity to isolation and a cell wall resynthesis in protoplast culture of recalcitrant yellow lupin |
| topic | cell wall fabaceae lupinus luteus l. protoplast culture viability |
| url | http://jcea.agr.hr/articles/774634_Explant_dependent_receptivity_to_isolation_and_a_cell_wall_resynthesis_in_protoplast_culture_of_recalcitrant_yellow_lupin_en.pdf |
| work_keys_str_mv | AT alinawiszniewska explantdependentreceptivitytoisolationandacellwallresynthesisinprotoplastcultureofrecalcitrantyellowlupin AT annapindel explantdependentreceptivitytoisolationandacellwallresynthesisinprotoplastcultureofrecalcitrantyellowlupin |