HSV1 microRNAs in glioblastoma development: an in silico study
Abstract Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor. Recent findings highlighted the significance of viral microRNAs (miRs) in regulating post-transcriptional mRNA expression in various human conditions. Although HSV1 encodes viral miRs and affects the central nervous s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45249-2 |
| _version_ | 1797363387151155200 |
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| author | Mahdi Abdoli Shadbad Nima Hemmat Mahla Abdoli Shadbad Oronzo Brunetti Nicola Silvestris Behzad Baradaran |
| author_facet | Mahdi Abdoli Shadbad Nima Hemmat Mahla Abdoli Shadbad Oronzo Brunetti Nicola Silvestris Behzad Baradaran |
| author_sort | Mahdi Abdoli Shadbad |
| collection | DOAJ |
| description | Abstract Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor. Recent findings highlighted the significance of viral microRNAs (miRs) in regulating post-transcriptional mRNA expression in various human conditions. Although HSV1 encodes viral miRs and affects the central nervous system, no study investigated the roles of HSV1-encoding miRs in GBM development. This study applied in silico approaches to investigate whether HSV1-encoding miRs are involved in GBM development and, if so, how they regulate tumor-suppressive/oncogenes expression in GBM. This study leveraged bioinformatics approaches to identify the potential effect of HSV1 miRs in GBM development. The GSE158284, GSE153679, and GSE182109 datasets were analyzed to identify differentially expressed genes in GBM tissues and cell lines using the limma package in the R software. The GSE182109 dataset was analyzed to determine gene expression at the single-cell levels using the Seurat package in the R software. The TCGA-GTEX, GDSC, CTRP, immunogenetic, and enrichment analyses were performed to study the impact of identified viral HSV1 miRs targets in GBM development. hsv1-miR-H6-3p is upregulated in GBM and can be responsible for EPB41L1 and SH3PXD2A downregulation in GBM tissues. Also, hsv1-miR-H1-5p is upregulated in GBM and can decrease the expression of MELK, FZD2, NOVA1, TMEM97, PTPRZ1, and PDGFC in GBM development. The single-cell RNA sequencing analyses have demonstrated that MELK, FZD2, NOVA1, TMEM97, PTPRZ1, and PDGFC are expressed in astrocytes residing in the GBM microenvironment. This study provides novel insights into the potential roles of HSV1 miRs in GBM pathogenesis and offers a reference for further studies on the significance of HSV1 miRs in GBM development. |
| first_indexed | 2024-03-08T16:19:42Z |
| format | Article |
| id | doaj.art-a16f08cc7b87412ea96a9a36f0a43143 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T16:19:42Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a16f08cc7b87412ea96a9a36f0a431432024-01-07T12:25:42ZengNature PortfolioScientific Reports2045-23222024-01-0114111210.1038/s41598-023-45249-2HSV1 microRNAs in glioblastoma development: an in silico studyMahdi Abdoli Shadbad0Nima Hemmat1Mahla Abdoli Shadbad2Oronzo Brunetti3Nicola Silvestris4Behzad Baradaran5Student Research Committee, Tabriz University of Medical SciencesImmunology Research Center, Tabriz University of Medical SciencesImmunology Research Center, Tabriz University of Medical SciencesMedical Oncology Unit-IRCCS Istituto Tumori “Giovanni Paolo II” of BariMedical Oncology Unit, Department of Human Pathology “G. Barresi”, University of MessinaImmunology Research Center, Tabriz University of Medical SciencesAbstract Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor. Recent findings highlighted the significance of viral microRNAs (miRs) in regulating post-transcriptional mRNA expression in various human conditions. Although HSV1 encodes viral miRs and affects the central nervous system, no study investigated the roles of HSV1-encoding miRs in GBM development. This study applied in silico approaches to investigate whether HSV1-encoding miRs are involved in GBM development and, if so, how they regulate tumor-suppressive/oncogenes expression in GBM. This study leveraged bioinformatics approaches to identify the potential effect of HSV1 miRs in GBM development. The GSE158284, GSE153679, and GSE182109 datasets were analyzed to identify differentially expressed genes in GBM tissues and cell lines using the limma package in the R software. The GSE182109 dataset was analyzed to determine gene expression at the single-cell levels using the Seurat package in the R software. The TCGA-GTEX, GDSC, CTRP, immunogenetic, and enrichment analyses were performed to study the impact of identified viral HSV1 miRs targets in GBM development. hsv1-miR-H6-3p is upregulated in GBM and can be responsible for EPB41L1 and SH3PXD2A downregulation in GBM tissues. Also, hsv1-miR-H1-5p is upregulated in GBM and can decrease the expression of MELK, FZD2, NOVA1, TMEM97, PTPRZ1, and PDGFC in GBM development. The single-cell RNA sequencing analyses have demonstrated that MELK, FZD2, NOVA1, TMEM97, PTPRZ1, and PDGFC are expressed in astrocytes residing in the GBM microenvironment. This study provides novel insights into the potential roles of HSV1 miRs in GBM pathogenesis and offers a reference for further studies on the significance of HSV1 miRs in GBM development.https://doi.org/10.1038/s41598-023-45249-2 |
| spellingShingle | Mahdi Abdoli Shadbad Nima Hemmat Mahla Abdoli Shadbad Oronzo Brunetti Nicola Silvestris Behzad Baradaran HSV1 microRNAs in glioblastoma development: an in silico study Scientific Reports |
| title | HSV1 microRNAs in glioblastoma development: an in silico study |
| title_full | HSV1 microRNAs in glioblastoma development: an in silico study |
| title_fullStr | HSV1 microRNAs in glioblastoma development: an in silico study |
| title_full_unstemmed | HSV1 microRNAs in glioblastoma development: an in silico study |
| title_short | HSV1 microRNAs in glioblastoma development: an in silico study |
| title_sort | hsv1 micrornas in glioblastoma development an in silico study |
| url | https://doi.org/10.1038/s41598-023-45249-2 |
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