Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria
Background: <i>Escherichia coli</i> and <i>Salmonella</i> are etiologic agents of intestinal infections. A previous study showed the presence of shared epitopes between lipopolysaccharides (LPSs) of <i>E. coli</i> O157 and <i>Salmonella</i>. Aim: Using...
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MDPI AG
2020-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/5/780 |
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| author | Armando Navarro Delia Licona-Moreno Alejandro Monsalvo-Reyes Ulises Hernández-Chiñas Carlos A. Eslava-Campos |
| author_facet | Armando Navarro Delia Licona-Moreno Alejandro Monsalvo-Reyes Ulises Hernández-Chiñas Carlos A. Eslava-Campos |
| author_sort | Armando Navarro |
| collection | DOAJ |
| description | Background: <i>Escherichia coli</i> and <i>Salmonella</i> are etiologic agents of intestinal infections. A previous study showed the presence of shared epitopes between lipopolysaccharides (LPSs) of <i>E. coli</i> O157 and <i>Salmonella</i>. Aim: Using phage display, the aim of this study is to identify mimotopes of shared epitopes in different enterobacterial LPSs. Methods: We use anti-LPS IgG from <i>E. coli</i> O157 and <i>Salmonella</i> to select peptide mimotopes of the M13 phage. The amino acid sequence of the mimotopes is used to synthesize peptides, which are in turn used to immunize rabbits. The antibody response of the resulting sera against the LPSs and synthetic peptides (SPs) is analyzed by ELISA and by Western blot assays, indicating that LPS sites are recognized by the same antibody. In a complementary test, the reactions of human serum samples obtained from the general population against the SPs and LPSs are also analyzed. Results: From the last biopanning phase, sixty phagotopes are selected. The analysis of the peptide mimotope amino acid sequences shows that in 4 of them the S/N/A/PF motif is a common sequence. Antibodies from the sera of immunized rabbits with SP287/3, SP459/1, SP308/3, and SP073/14 react against both their own peptide and the different LPSs. The Western blot test shows a sera reaction against both the lateral chains and the cores of the LPSs. The analysis of the human sera shows a response against the SPs and LPSs. Conclusion: The designed synthetic peptides are mimotopes of LPS epitopes of <i>Salmonella</i> and <i>E. coli</i> that possess immunogenic capacity. These mimotopes could be considered for use in the design of vaccines against both enterobacteria. |
| first_indexed | 2024-03-10T19:39:56Z |
| format | Article |
| id | doaj.art-a16fa2e7298d45619769cab520f4fe0c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:39:56Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a16fa2e7298d45619769cab520f4fe0c2023-11-20T01:22:11ZengMDPI AGMicroorganisms2076-26072020-05-018578010.3390/microorganisms8050780Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant EnterobacteriaArmando Navarro0Delia Licona-Moreno1Alejandro Monsalvo-Reyes2Ulises Hernández-Chiñas3Carlos A. Eslava-Campos4Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, México City CP 04510, MexicoDepartamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, México City CP 04510, MexicoLaboratorio de Bioquímica Molecular, Unidad de Biología y Prototipos, UBIPRO, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Los Reyes Ixtacala Tlalnepantla, Estado de México P C 54090, MexicoDepartamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, México City CP 04510, MexicoDepartamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, México City CP 04510, MexicoBackground: <i>Escherichia coli</i> and <i>Salmonella</i> are etiologic agents of intestinal infections. A previous study showed the presence of shared epitopes between lipopolysaccharides (LPSs) of <i>E. coli</i> O157 and <i>Salmonella</i>. Aim: Using phage display, the aim of this study is to identify mimotopes of shared epitopes in different enterobacterial LPSs. Methods: We use anti-LPS IgG from <i>E. coli</i> O157 and <i>Salmonella</i> to select peptide mimotopes of the M13 phage. The amino acid sequence of the mimotopes is used to synthesize peptides, which are in turn used to immunize rabbits. The antibody response of the resulting sera against the LPSs and synthetic peptides (SPs) is analyzed by ELISA and by Western blot assays, indicating that LPS sites are recognized by the same antibody. In a complementary test, the reactions of human serum samples obtained from the general population against the SPs and LPSs are also analyzed. Results: From the last biopanning phase, sixty phagotopes are selected. The analysis of the peptide mimotope amino acid sequences shows that in 4 of them the S/N/A/PF motif is a common sequence. Antibodies from the sera of immunized rabbits with SP287/3, SP459/1, SP308/3, and SP073/14 react against both their own peptide and the different LPSs. The Western blot test shows a sera reaction against both the lateral chains and the cores of the LPSs. The analysis of the human sera shows a response against the SPs and LPSs. Conclusion: The designed synthetic peptides are mimotopes of LPS epitopes of <i>Salmonella</i> and <i>E. coli</i> that possess immunogenic capacity. These mimotopes could be considered for use in the design of vaccines against both enterobacteria.https://www.mdpi.com/2076-2607/8/5/780mimotopesepitopes<i>Salmonella</i><i>E. coli</i>lipopolysaccharidephagotopes |
| spellingShingle | Armando Navarro Delia Licona-Moreno Alejandro Monsalvo-Reyes Ulises Hernández-Chiñas Carlos A. Eslava-Campos Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria Microorganisms mimotopes epitopes <i>Salmonella</i> <i>E. coli</i> lipopolysaccharide phagotopes |
| title | Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria |
| title_full | Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria |
| title_fullStr | Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria |
| title_full_unstemmed | Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria |
| title_short | Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria |
| title_sort | phage display detection of mimotopes that are shared epitopes of clinically and epidemiologically relevant enterobacteria |
| topic | mimotopes epitopes <i>Salmonella</i> <i>E. coli</i> lipopolysaccharide phagotopes |
| url | https://www.mdpi.com/2076-2607/8/5/780 |
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