Preparation, Characterization and Optimization of Etoposide-Loaded Gold Nanoparticles Based on Chemical Reduction Method
In recent years, observed focus greatly on gold nanoparticles synthesis due to its unique properties and tremendous applicability. In most of these researches, the citrate reduction method has been adopted. The aim of this study was to prepare and optimize monodisperse ultrafine particles by additio...
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| Format: | Article |
| Language: | English |
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College of Pharmacy University of Baghdad
2020-12-01
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| Series: | Iraqi Journal of Pharmaceutical Sciences |
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| Online Access: | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1069 |
| _version_ | 1818567603585548288 |
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| author | Maha M Ali Nawal A. Rajab Alaa A. Abdulrasool |
| author_facet | Maha M Ali Nawal A. Rajab Alaa A. Abdulrasool |
| author_sort | Maha M Ali |
| collection | DOAJ |
| description | In recent years, observed focus greatly on gold nanoparticles synthesis due to its unique properties and tremendous applicability. In most of these researches, the citrate reduction method has been adopted. The aim of this study was to prepare and optimize monodisperse ultrafine particles by addition of reducing agent to gold salt, as a result of seed mediated growth mechanism. In this research, gold nanoparticles suspension (G) was prepared by traditional standard Turkevich method and optimized by studying different variables such as reactants concentrations, preparation temperature and stirring rate on controlling size and uniformity of nanoparticles through preparing twenty formulas (G1-G20). Subsequently, the selected formula that prepared from the best tested condition was further optimized by preparing it using inverse method via the addition of gold salt to the reducing agent in opposite to the previous traditional method (G21). The optimized gold nanoparticles were characterized by SEM, EDX, TEM and zeta potential. The obtained results indicated that (G21) with reactants concentrations of 0.5mM and 10mM for HAuCl4.3H2O and trisodium citrate dihydrate respectively, 65°C of preparation temperature and 1500rpm of stirring rate was chosen as an optimized formula according to AFM provided gold nanoparticles with smoother surface, smaller size (average 8.75nm) with more uniform size distribution (7.32%) as well as short over all preparation time (27minutes). In addition to that all results of SEM, EDX and TEM indicated uniform spherical shape with zeta potential of -47.87. In conclusion, inversed method is promising for the preparation of gold nanoparticles with high monodispersity. |
| first_indexed | 2024-12-14T06:25:30Z |
| format | Article |
| id | doaj.art-a170a4a6dd8847279569c9d83f7862b7 |
| institution | Directory Open Access Journal |
| issn | 2521-3512 1683-3597 |
| language | English |
| last_indexed | 2024-12-14T06:25:30Z |
| publishDate | 2020-12-01 |
| publisher | College of Pharmacy University of Baghdad |
| record_format | Article |
| series | Iraqi Journal of Pharmaceutical Sciences |
| spelling | doaj.art-a170a4a6dd8847279569c9d83f7862b72022-12-21T23:13:42ZengCollege of Pharmacy University of BaghdadIraqi Journal of Pharmaceutical Sciences2521-35121683-35972020-12-0129210.31351/vol29iss2pp107-1211069Preparation, Characterization and Optimization of Etoposide-Loaded Gold Nanoparticles Based on Chemical Reduction MethodMaha M Ali0Nawal A. RajabAlaa A. AbdulrasoolCollege of pharmacy,University of BaghdadIn recent years, observed focus greatly on gold nanoparticles synthesis due to its unique properties and tremendous applicability. In most of these researches, the citrate reduction method has been adopted. The aim of this study was to prepare and optimize monodisperse ultrafine particles by addition of reducing agent to gold salt, as a result of seed mediated growth mechanism. In this research, gold nanoparticles suspension (G) was prepared by traditional standard Turkevich method and optimized by studying different variables such as reactants concentrations, preparation temperature and stirring rate on controlling size and uniformity of nanoparticles through preparing twenty formulas (G1-G20). Subsequently, the selected formula that prepared from the best tested condition was further optimized by preparing it using inverse method via the addition of gold salt to the reducing agent in opposite to the previous traditional method (G21). The optimized gold nanoparticles were characterized by SEM, EDX, TEM and zeta potential. The obtained results indicated that (G21) with reactants concentrations of 0.5mM and 10mM for HAuCl4.3H2O and trisodium citrate dihydrate respectively, 65°C of preparation temperature and 1500rpm of stirring rate was chosen as an optimized formula according to AFM provided gold nanoparticles with smoother surface, smaller size (average 8.75nm) with more uniform size distribution (7.32%) as well as short over all preparation time (27minutes). In addition to that all results of SEM, EDX and TEM indicated uniform spherical shape with zeta potential of -47.87. In conclusion, inversed method is promising for the preparation of gold nanoparticles with high monodispersity.https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1069Gold Nanoparticles, Etoposide, Classical and Inversed Citrate Reduction Method. |
| spellingShingle | Maha M Ali Nawal A. Rajab Alaa A. Abdulrasool Preparation, Characterization and Optimization of Etoposide-Loaded Gold Nanoparticles Based on Chemical Reduction Method Iraqi Journal of Pharmaceutical Sciences Gold Nanoparticles, Etoposide, Classical and Inversed Citrate Reduction Method. |
| title | Preparation, Characterization and Optimization of Etoposide-Loaded Gold Nanoparticles Based on Chemical Reduction Method |
| title_full | Preparation, Characterization and Optimization of Etoposide-Loaded Gold Nanoparticles Based on Chemical Reduction Method |
| title_fullStr | Preparation, Characterization and Optimization of Etoposide-Loaded Gold Nanoparticles Based on Chemical Reduction Method |
| title_full_unstemmed | Preparation, Characterization and Optimization of Etoposide-Loaded Gold Nanoparticles Based on Chemical Reduction Method |
| title_short | Preparation, Characterization and Optimization of Etoposide-Loaded Gold Nanoparticles Based on Chemical Reduction Method |
| title_sort | preparation characterization and optimization of etoposide loaded gold nanoparticles based on chemical reduction method |
| topic | Gold Nanoparticles, Etoposide, Classical and Inversed Citrate Reduction Method. |
| url | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1069 |
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