Seaweed <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i> Pigments: Anti-Dermatophytic Activity
Seaweeds have been explored as a natural resource of compounds of interest due to their bioactivities. Although many studies report the interest and the application of seaweeds in various areas, from food or human health to the economy, these data mostly focus on raw extracts and not on specific com...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/14/4/1456 |
| _version_ | 1797299040132530176 |
|---|---|
| author | Louisa Gomes João Cotas Chantal Fernandes Teresa Gonçalves Leonel Pereira |
| author_facet | Louisa Gomes João Cotas Chantal Fernandes Teresa Gonçalves Leonel Pereira |
| author_sort | Louisa Gomes |
| collection | DOAJ |
| description | Seaweeds have been explored as a natural resource of compounds of interest due to their bioactivities. Although many studies report the interest and the application of seaweeds in various areas, from food or human health to the economy, these data mostly focus on raw extracts and not on specific compounds, such as seaweed pigments. Fungal infections of the skin, nails, and hair caused by dermatophytes are the most common fungal infections worldwide. These pathologies require long periods of topical and/or systemic treatment associated with adverse effects and increased antifungal resistance. So, this study had two objectives: the first was to isolate and characterize the pigments of the seaweeds <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i>; the second was to assess their antifungal activity. The extraction of pigments was performed using a method of extraction by exhaustion, and the purification was achieved via column chromatography. Three techniques were used to characterize the pigments: thin-layer chromatography (TLC), UV–visible spectrophotometry, and Fourier-transform infrared spectroscopy (FTIR). The antifungal activity against the three most common dermatophytes, <i>Trichophyton rubrum</i>, <i>Trichophyton mentagrophytes</i>, and <i>Microsporum canis</i>, was evaluated using a microdilution methodology, following the EUCAST international standards. It was possible to observe that the extracts obtained from the seaweed <i>C. jubata</i>, corresponding to the purified pigment phycobiliprotein, and the crude extract (an enriched extract) showed antifungal activity against the three fungal agents of human skin infection. |
| first_indexed | 2024-03-07T22:43:46Z |
| format | Article |
| id | doaj.art-a171507b64434b06b6c5009daa2affb6 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-07T22:43:46Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-a171507b64434b06b6c5009daa2affb62024-02-23T15:06:02ZengMDPI AGApplied Sciences2076-34172024-02-01144145610.3390/app14041456Seaweed <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i> Pigments: Anti-Dermatophytic ActivityLouisa Gomes0João Cotas1Chantal Fernandes2Teresa Gonçalves3Leonel Pereira4Marine Resources, Conservation and Technology, Marine Algae Lab, CFE—Centre for Functional Ecology, Science for People & Planet, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, PortugalMarine Resources, Conservation and Technology, Marine Algae Lab, CFE—Centre for Functional Ecology, Science for People & Planet, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, PortugalCNC—Center for Neurosciences and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, PortugalCNC—Center for Neurosciences and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, PortugalMarine Resources, Conservation and Technology, Marine Algae Lab, CFE—Centre for Functional Ecology, Science for People & Planet, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, PortugalSeaweeds have been explored as a natural resource of compounds of interest due to their bioactivities. Although many studies report the interest and the application of seaweeds in various areas, from food or human health to the economy, these data mostly focus on raw extracts and not on specific compounds, such as seaweed pigments. Fungal infections of the skin, nails, and hair caused by dermatophytes are the most common fungal infections worldwide. These pathologies require long periods of topical and/or systemic treatment associated with adverse effects and increased antifungal resistance. So, this study had two objectives: the first was to isolate and characterize the pigments of the seaweeds <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i>; the second was to assess their antifungal activity. The extraction of pigments was performed using a method of extraction by exhaustion, and the purification was achieved via column chromatography. Three techniques were used to characterize the pigments: thin-layer chromatography (TLC), UV–visible spectrophotometry, and Fourier-transform infrared spectroscopy (FTIR). The antifungal activity against the three most common dermatophytes, <i>Trichophyton rubrum</i>, <i>Trichophyton mentagrophytes</i>, and <i>Microsporum canis</i>, was evaluated using a microdilution methodology, following the EUCAST international standards. It was possible to observe that the extracts obtained from the seaweed <i>C. jubata</i>, corresponding to the purified pigment phycobiliprotein, and the crude extract (an enriched extract) showed antifungal activity against the three fungal agents of human skin infection.https://www.mdpi.com/2076-3417/14/4/1456seaweedspigmentsnatural extractsantifungal activityUV spectrophotometryTLC |
| spellingShingle | Louisa Gomes João Cotas Chantal Fernandes Teresa Gonçalves Leonel Pereira Seaweed <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i> Pigments: Anti-Dermatophytic Activity Applied Sciences seaweeds pigments natural extracts antifungal activity UV spectrophotometry TLC |
| title | Seaweed <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i> Pigments: Anti-Dermatophytic Activity |
| title_full | Seaweed <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i> Pigments: Anti-Dermatophytic Activity |
| title_fullStr | Seaweed <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i> Pigments: Anti-Dermatophytic Activity |
| title_full_unstemmed | Seaweed <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i> Pigments: Anti-Dermatophytic Activity |
| title_short | Seaweed <i>Calliblepharis jubata</i> and <i>Fucus vesiculosus</i> Pigments: Anti-Dermatophytic Activity |
| title_sort | seaweed i calliblepharis jubata i and i fucus vesiculosus i pigments anti dermatophytic activity |
| topic | seaweeds pigments natural extracts antifungal activity UV spectrophotometry TLC |
| url | https://www.mdpi.com/2076-3417/14/4/1456 |
| work_keys_str_mv | AT louisagomes seaweedicalliblepharisjubataiandifucusvesiculosusipigmentsantidermatophyticactivity AT joaocotas seaweedicalliblepharisjubataiandifucusvesiculosusipigmentsantidermatophyticactivity AT chantalfernandes seaweedicalliblepharisjubataiandifucusvesiculosusipigmentsantidermatophyticactivity AT teresagoncalves seaweedicalliblepharisjubataiandifucusvesiculosusipigmentsantidermatophyticactivity AT leonelpereira seaweedicalliblepharisjubataiandifucusvesiculosusipigmentsantidermatophyticactivity |