Genotype Distribution and 5’ UTR Nucleotide Changes in Hepatitis C Virus
Objective: HCV is a single-stranded RNA virus which has 9500 nucleotide. On the 3’ and 5’ ends of the genome, there are two untranslated regions (UTR) which are highly protected and which have 92% homology among various HCV types. 5’ UTR is used for the genotype detection. In this study, genotype an...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2011-09-01
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| Series: | Balkan Medical Journal |
| Subjects: | |
| Online Access: | http://tutfd.org/text.php3?id=768 |
| _version_ | 1828017158531055616 |
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| author | Tekin Karslıgil Eda Savaş M. Cemil Savaş |
| author_facet | Tekin Karslıgil Eda Savaş M. Cemil Savaş |
| author_sort | Tekin Karslıgil |
| collection | DOAJ |
| description | Objective: HCV is a single-stranded RNA virus which has 9500 nucleotide. On the 3’ and 5’ ends of the genome, there are two untranslated regions (UTR) which are highly protected and which have 92% homology among various HCV types. 5’ UTR is used for the genotype detection. In this study, genotype and nucleic acid changes in this region were analyzed.Material and Methods: In 51 patients diagnosed with HCV, HCV-RNA was isolated and purified from serum samples. The 341 nucleotide-long UTR region at the 5’end of the genome was sequenced and genotypes were detected. Nucleotide changes were analyzed with on-line BLAST program.Results: In 45 (88.2%) of 51 patients, genotype-1 (78.4% of all genotypes were 1b, 9.8% were 1a) was detected. In 41 (80.4%) of the 51 sequences, nucleic acid changes were detected. These changes generally occurred as an insertion in codon 84 ; deletion in codon 43 and codon 46; transversion in codon 15, 17 and 18, and transition in codon 62. No significant relationship was found between viral load and nucleic acid changes. Conclusion: Although the 5’UTR region is a protected region, mutation can be observed, and the mutations may affect the genotype, viral load and treatment response. Therefore, further investigation is required in a large series. |
| first_indexed | 2024-04-10T10:45:42Z |
| format | Article |
| id | doaj.art-a1715407d8ef4e729cd664ecc15b766b |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T10:45:42Z |
| publishDate | 2011-09-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-a1715407d8ef4e729cd664ecc15b766b2023-02-15T16:20:19ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312011-09-012803232236Genotype Distribution and 5’ UTR Nucleotide Changes in Hepatitis C VirusTekin KarslıgilEda SavaşM. Cemil SavaşObjective: HCV is a single-stranded RNA virus which has 9500 nucleotide. On the 3’ and 5’ ends of the genome, there are two untranslated regions (UTR) which are highly protected and which have 92% homology among various HCV types. 5’ UTR is used for the genotype detection. In this study, genotype and nucleic acid changes in this region were analyzed.Material and Methods: In 51 patients diagnosed with HCV, HCV-RNA was isolated and purified from serum samples. The 341 nucleotide-long UTR region at the 5’end of the genome was sequenced and genotypes were detected. Nucleotide changes were analyzed with on-line BLAST program.Results: In 45 (88.2%) of 51 patients, genotype-1 (78.4% of all genotypes were 1b, 9.8% were 1a) was detected. In 41 (80.4%) of the 51 sequences, nucleic acid changes were detected. These changes generally occurred as an insertion in codon 84 ; deletion in codon 43 and codon 46; transversion in codon 15, 17 and 18, and transition in codon 62. No significant relationship was found between viral load and nucleic acid changes. Conclusion: Although the 5’UTR region is a protected region, mutation can be observed, and the mutations may affect the genotype, viral load and treatment response. Therefore, further investigation is required in a large series.http://tutfd.org/text.php3?id=768HCVGenotyping5’ UTR nucleotid changes |
| spellingShingle | Tekin Karslıgil Eda Savaş M. Cemil Savaş Genotype Distribution and 5’ UTR Nucleotide Changes in Hepatitis C Virus Balkan Medical Journal HCV Genotyping 5’ UTR nucleotid changes |
| title | Genotype Distribution and 5’ UTR Nucleotide Changes in Hepatitis C Virus |
| title_full | Genotype Distribution and 5’ UTR Nucleotide Changes in Hepatitis C Virus |
| title_fullStr | Genotype Distribution and 5’ UTR Nucleotide Changes in Hepatitis C Virus |
| title_full_unstemmed | Genotype Distribution and 5’ UTR Nucleotide Changes in Hepatitis C Virus |
| title_short | Genotype Distribution and 5’ UTR Nucleotide Changes in Hepatitis C Virus |
| title_sort | genotype distribution and 5 utr nucleotide changes in hepatitis c virus |
| topic | HCV Genotyping 5’ UTR nucleotid changes |
| url | http://tutfd.org/text.php3?id=768 |
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