Expression of a Stilbene Synthase Gene from the <i>Vitis labrusca</i> x <i>Vitis vinifera</i> L. Hybrid Increases the Resistance of Transgenic <i>Nicotiana tabacum</i> L. Plants to <i>Erwinia carotovora</i>
‘Isabel’ grape (<i>Vitis labrusca</i> x <i>V. vinifera</i> L. hybrid) is one of the main grape cultivars in Russia and some other countries for processing, due to its vigor, tolerance to the main fungal diseases, high yield and potential for sugar accumulation. The stilbene s...
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2022-03-01
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| author | Elena B. Rukavtsova Valeriya V. Alekseeva Sergey V. Tarlachkov Natalia S. Zakharchenko Alexander A. Ermoshin Svetlana A. Zimnitskaya Alexey K. Surin Elena Y. Gorbunova Viatcheslav N. Azev Sergey S. Sheshnitsan Konstantin A. Shestibratov Yaroslav I. Buryanov |
| author_facet | Elena B. Rukavtsova Valeriya V. Alekseeva Sergey V. Tarlachkov Natalia S. Zakharchenko Alexander A. Ermoshin Svetlana A. Zimnitskaya Alexey K. Surin Elena Y. Gorbunova Viatcheslav N. Azev Sergey S. Sheshnitsan Konstantin A. Shestibratov Yaroslav I. Buryanov |
| author_sort | Elena B. Rukavtsova |
| collection | DOAJ |
| description | ‘Isabel’ grape (<i>Vitis labrusca</i> x <i>V. vinifera</i> L. hybrid) is one of the main grape cultivars in Russia and some other countries for processing, due to its vigor, tolerance to the main fungal diseases, high yield and potential for sugar accumulation. The stilbene synthase gene <i>VlvSTS</i> was isolated from the hybrid grape cv. Isabel and cloned into a pSS plant transformation vector under the control of a constitutive 35S RNA double promoter of the cauliflower mosaic virus, CaMV 35SS. <i>VlvSTS</i>-gene containing transgenic tobacco lines were obtained and analyzed. For the first time plants expressing the <i>VlvSTS</i> gene were shown to have an enhanced resistance to the bacterial pathogen <i>Erwinia carotovora</i> subsp. <i>carotovora</i> B15. Transgenic plants were tested for resistance to a number of fungal pathogens. The plants were resistant to the grey mould fungus <i>Botrytis cinerea</i>, but not to the fungi <i>Fusarium oxysporum</i>, <i>F. sporotrichioides</i>, or <i>F. culmorum</i>. According to the results of a high performance liquid chromatography-mass spectrometry analysis, the amount of trans-resveratrol in leaves of transgenic plants with the highest expression of the <i>VlvSTS</i> gene was in a range from 150 to 170 μg/g of raw biomass. Change in the color and a decreased anthocyanin content in the flower corollas of transgenic plants were observed in transgenic lines with the highest expression of <i>VlvSTS</i>. A decrease in total flavonoid content was found in the flower petals but not the leaves of these tobacco lines. High expression of the <i>VlvSTS</i> gene influenced pollen development and seed productivity in transgenic plants. The size of pollen grains increased, while their total number per anther decreased. A decrease in the number of fertile pollen grains resulted in a decreased average weight of a seed boll in transgenic plants. |
| first_indexed | 2024-03-09T12:56:08Z |
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| language | English |
| last_indexed | 2024-03-09T12:56:08Z |
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| spelling | doaj.art-a17da8a205d84e079ed023a698b715172023-11-30T22:00:49ZengMDPI AGPlants2223-77472022-03-0111677010.3390/plants11060770Expression of a Stilbene Synthase Gene from the <i>Vitis labrusca</i> x <i>Vitis vinifera</i> L. Hybrid Increases the Resistance of Transgenic <i>Nicotiana tabacum</i> L. Plants to <i>Erwinia carotovora</i>Elena B. Rukavtsova0Valeriya V. Alekseeva1Sergey V. Tarlachkov2Natalia S. Zakharchenko3Alexander A. Ermoshin4Svetlana A. Zimnitskaya5Alexey K. Surin6Elena Y. Gorbunova7Viatcheslav N. Azev8Sergey S. Sheshnitsan9Konstantin A. Shestibratov10Yaroslav I. Buryanov11Department of Biomolecular Chemistry, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaDepartment of Plant Molecular Biology and Biotechnology, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaDepartment of Biotechnology, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaDepartment of Biomolecular Chemistry, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaDepartment of Experimental Biology and Biotechnology, Institute of Natural Science and Mathematics, Ural Federal University, 620002 Ekaterinburg, Sverdlovsk Region, RussiaDepartment of Biology and Fundamental Medicine, Institute of Natural Science and Mathematics, Ural Federal University, 620002 Ekaterinburg, Sverdlovsk Region, RussiaDepartment of Biological Testing, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaDepartment of Biotechnology, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaDepartment of Biotechnology, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaFaculty of Forestry, Voronezh State University of Forestry and Technologies Named after G.F. Morozov, 8 Timiryazeva Str., 394087 Voronezh, Voronezh Region, RussiaDepartment of Plant Molecular Biology and Biotechnology, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaDepartment of Plant Molecular Biology and Biotechnology, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia‘Isabel’ grape (<i>Vitis labrusca</i> x <i>V. vinifera</i> L. hybrid) is one of the main grape cultivars in Russia and some other countries for processing, due to its vigor, tolerance to the main fungal diseases, high yield and potential for sugar accumulation. The stilbene synthase gene <i>VlvSTS</i> was isolated from the hybrid grape cv. Isabel and cloned into a pSS plant transformation vector under the control of a constitutive 35S RNA double promoter of the cauliflower mosaic virus, CaMV 35SS. <i>VlvSTS</i>-gene containing transgenic tobacco lines were obtained and analyzed. For the first time plants expressing the <i>VlvSTS</i> gene were shown to have an enhanced resistance to the bacterial pathogen <i>Erwinia carotovora</i> subsp. <i>carotovora</i> B15. Transgenic plants were tested for resistance to a number of fungal pathogens. The plants were resistant to the grey mould fungus <i>Botrytis cinerea</i>, but not to the fungi <i>Fusarium oxysporum</i>, <i>F. sporotrichioides</i>, or <i>F. culmorum</i>. According to the results of a high performance liquid chromatography-mass spectrometry analysis, the amount of trans-resveratrol in leaves of transgenic plants with the highest expression of the <i>VlvSTS</i> gene was in a range from 150 to 170 μg/g of raw biomass. Change in the color and a decreased anthocyanin content in the flower corollas of transgenic plants were observed in transgenic lines with the highest expression of <i>VlvSTS</i>. A decrease in total flavonoid content was found in the flower petals but not the leaves of these tobacco lines. High expression of the <i>VlvSTS</i> gene influenced pollen development and seed productivity in transgenic plants. The size of pollen grains increased, while their total number per anther decreased. A decrease in the number of fertile pollen grains resulted in a decreased average weight of a seed boll in transgenic plants.https://www.mdpi.com/2223-7747/11/6/770<i>Nicotiana tabacum</i> L.stilbene synthasetransgenic plantspathogensresveratrolflavonoids |
| spellingShingle | Elena B. Rukavtsova Valeriya V. Alekseeva Sergey V. Tarlachkov Natalia S. Zakharchenko Alexander A. Ermoshin Svetlana A. Zimnitskaya Alexey K. Surin Elena Y. Gorbunova Viatcheslav N. Azev Sergey S. Sheshnitsan Konstantin A. Shestibratov Yaroslav I. Buryanov Expression of a Stilbene Synthase Gene from the <i>Vitis labrusca</i> x <i>Vitis vinifera</i> L. Hybrid Increases the Resistance of Transgenic <i>Nicotiana tabacum</i> L. Plants to <i>Erwinia carotovora</i> Plants <i>Nicotiana tabacum</i> L. stilbene synthase transgenic plants pathogens resveratrol flavonoids |
| title | Expression of a Stilbene Synthase Gene from the <i>Vitis labrusca</i> x <i>Vitis vinifera</i> L. Hybrid Increases the Resistance of Transgenic <i>Nicotiana tabacum</i> L. Plants to <i>Erwinia carotovora</i> |
| title_full | Expression of a Stilbene Synthase Gene from the <i>Vitis labrusca</i> x <i>Vitis vinifera</i> L. Hybrid Increases the Resistance of Transgenic <i>Nicotiana tabacum</i> L. Plants to <i>Erwinia carotovora</i> |
| title_fullStr | Expression of a Stilbene Synthase Gene from the <i>Vitis labrusca</i> x <i>Vitis vinifera</i> L. Hybrid Increases the Resistance of Transgenic <i>Nicotiana tabacum</i> L. Plants to <i>Erwinia carotovora</i> |
| title_full_unstemmed | Expression of a Stilbene Synthase Gene from the <i>Vitis labrusca</i> x <i>Vitis vinifera</i> L. Hybrid Increases the Resistance of Transgenic <i>Nicotiana tabacum</i> L. Plants to <i>Erwinia carotovora</i> |
| title_short | Expression of a Stilbene Synthase Gene from the <i>Vitis labrusca</i> x <i>Vitis vinifera</i> L. Hybrid Increases the Resistance of Transgenic <i>Nicotiana tabacum</i> L. Plants to <i>Erwinia carotovora</i> |
| title_sort | expression of a stilbene synthase gene from the i vitis labrusca i x i vitis vinifera i l hybrid increases the resistance of transgenic i nicotiana tabacum i l plants to i erwinia carotovora i |
| topic | <i>Nicotiana tabacum</i> L. stilbene synthase transgenic plants pathogens resveratrol flavonoids |
| url | https://www.mdpi.com/2223-7747/11/6/770 |
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